Real-time detection of ochratoxin A in wine through insight of aptamer conformation in conjunction with graphene field-effect transistor

Nekrasov, Nikita; Jaric, Stefan; Kireev, Dmitry; Emelianov, Aleksei V.; Орлов, А. В.; Gadjanski, Ivana; Никитин, П. И.; Akinwande, Deji; Bobrinetskiy, Ivan

doi:10.1016/j.bios.2021.113890

Cited by 52 publications

(35 citation statements)

References 42 publications

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“…The n-doping effect of the GFET was ascribed to the conformational change of the aptamer upon its specific binding to arsenite anions. ,− To explore the conformation transformation of the aptamer before and after binding to arsenite, we conducted CD spectroscopy studies, showing a prominent peak at around 280 nm. This result revealed the formation of a prominent duplex in the aptamer, which is consistent with the theoretical molecular dynamic (MD) study (Figure S5 of the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Aptasensors Based on Graphene Field-Effect Transistors for Arsenite Detection

Tyagi

Huang

et al. 2022

ACS Appl. Nano Mater.

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All-electronic chemical sensors based on field-effect transistors (FETs) hold tremendous promise for rapid and sensitive detection of arsenic in groundwater. Their sensitivity, however, is fundamentally limited by a chemical gating mechanism where target ion charges are directly measured. We have developed a highly sensitive aptasensor, based on scalable graphene FETs, which relies on the conformational change of the negatively charged aptamer upon arsenite binding, offering a wide analytical range, from 0.05 to 1000 ppb, and with a 0.02 ppb detection limit. Circular dichroism spectroscopy studies confirmed the conformational change of the aptamer, and molecular dynamic studies further validated that arsenite had bound with the hairpin loop of the aptamer. This work provides a highly accurate chemical sensing platform for rapid detection of arsenic in drinking water.

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Section: Resultsmentioning

confidence: 99%

Aptasensors Based on Graphene Field-Effect Transistors for Arsenite Detection

Tyagi

Huang

et al. 2022

ACS Appl. Nano Mater.

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“…The determination of specific DNA or RNA molecules is accomplished following the interaction with the nucleic acid (PNA or DNA) chain containing the complementary basis sequence and typical LOD values are in the range 10–100 fM [ 184 , 185 , 187 – 189 ]. The use of nucleic acids overcomes the simple detection of the complementary chains, thanks to the choice of aptamers, which have been exploited to sense cytokines [ 202 , 203 ], ochratoxin A [ 204 ], thrombin [ 205 ], and 17β-estradiol [ 206 ] with LOD values around 1 pM. The immunosensing approach has been exploited for the detection of specific biomarkers for Alzheimer [ 195 , 200 ], cancer [ 196 , 197 ], heart failure [ 199 ], cardiovascular disorders [ 198 ], and venous thrombosis [ 194 ].…”

Section: Micro- and Nano-sized Sensors Based On A Transistor Architec...mentioning

confidence: 99%

Micro- and nano-devices for electrochemical sensing

et al. 2022

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Electrode miniaturization has profoundly revolutionized the field of electrochemical sensing, opening up unprecedented opportunities for probing biological events with a high spatial and temporal resolution, integrating electrochemical systems with microfluidics, and designing arrays for multiplexed sensing. Several technological issues posed by the desire for downsizing have been addressed so far, leading to micrometric and nanometric sensing systems with different degrees of maturity. However, there is still an endless margin for researchers to improve current strategies and cope with demanding sensing fields, such as lab-on-a-chip devices and multi-array sensors, brain chemistry, and cell monitoring. In this review, we present current trends in the design of micro-/nano-electrochemical sensors and cutting-edge applications reported in the last 10 years. Micro- and nanosensors are divided into four categories depending on the transduction mechanism, e.g., amperometric, impedimetric, potentiometric, and transistor-based, to best guide the reader through the different detection strategies and highlight major advancements as well as still unaddressed demands in electrochemical sensing. Graphical Abstract

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“…Finally, there is no doubt that the best strategy is the prevention of contamination of raw materials with fungi. However, to date, total management in the field is not possible, and removal strategies are an alternative to mitigate the final problem, as long as they go hand in hand with adequate detection strategies [36].…”

Section: Optimization Of Ota and Phenol Removal By Aplp Complexesmentioning

confidence: 99%

Removal of Ochratoxin A from Red Wine Using Alginate-PVA-L. plantarum (APLP) Complexes: A Preliminary Study

Castro

Laurie

Padilla

et al. 2022

Toxins

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The presence of ochratoxin A (OTA) in wines is a problem mainly due to the health damage it can cause to frequent drinkers. A method for removing these toxic substances from wine is the use of lactic acid bacteria with mycotoxin-adsorption capacities; however, their use is limited since a matrix in which they can be immobilized, to remove them after use, is needed. In this study, L. plantarum (LP) was encapsulated in a polymeric matrix composed of polyvinyl alcohol (PVA) and alginate, forming alginate–PVA–LP (APLP) complexes. Then, these complexes were characterized, and assays of OTA and phenol removal from wines were performed. As a result, it was observed that the APLP complexes at a concentration of 0.5 g mL−1 removed over 50% of the OTA without substantially affecting the concentration of total phenols. In addition, it was determined that the presence of L. plantarum directly affected the ability to adsorb OTA from wines and did not decrease the total phenols. In conclusion, an alginate–PVA matrix allows immobilizing LP, and the complexes formed are an alternative for removing ochratoxin from contaminated wines.

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Real-time detection of ochratoxin A in wine through insight of aptamer conformation in conjunction with graphene field-effect transistor

Cited by 52 publications

References 42 publications

Aptasensors Based on Graphene Field-Effect Transistors for Arsenite Detection

Aptasensors Based on Graphene Field-Effect Transistors for Arsenite Detection

Micro- and nano-devices for electrochemical sensing

Removal of Ochratoxin A from Red Wine Using Alginate-PVA-L. plantarum (APLP) Complexes: A Preliminary Study

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