Real-Time Detection of the Bacterial Biofilm Formation Stages Using QCM-Based Sensors

Soler, Jordi; Amer, Miquel-Àngel; Turó, A.; Castro, Nagore; Navarro, Marc; Soto, Sara M.; Gabasa, Yaiza; López, Yuly; Chavez-Dominguez, Juan A.

doi:10.3390/chemosensors11010068

Cited by 8 publications

(3 citation statements)

References 35 publications

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“…An ideal monitoring device should offer fast, accurate, realtime, non-destructive, on-line, and cost-effective information about fouling layer development. To the best of our knowledge this ambitious goal has not been reached, but several techniques have been proposed, including nanovibration-based devices (Salazar et al, 2023) and spectroscopy technique for online biofilm monitoring (Kampouraki et al, 2024), nanoparticles probes for detection purpose (Bazsefidpar et al, 2023).…”

Section: "Promising Coating" Technologies: Nanoparticle and Peptidesmentioning

confidence: 99%

Biofilm contamination in confined space stations: reduction, coexistence or an opportunity?

Marra,

Ferraro,

Caserta

2024

Front. Mater.

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The prolonged human permanence in confined environments in space, such as in the case of the International Space Station, has inadvertently fostered conditions leading to uncontrolled microbial proliferation on surfaces, known as biofilm. Biofilm presence represents a challenge in critical spacecraft systems, that can lead to contamination issues and systems loss of function due to biofouling phenomena. This scenario is further complicated by microgravity that has a controversial role on biofilm growth and formation. Biocontamination can be a limiting factor in human long-term mission in outer Earth orbit and an economic and health issue on ISS. This study addresses the pressing need for effective antimicrobial strategies against such resilient biofilms in confined environments where the usage of biocidal chemical compounds is strictly controlled due to toxicity dangers. Traditional methods can be complemented by advanced antimicrobial coatings techniques. A promising approach is based on the oxygen plasma as coating platform. The technology can be potentially extended to a wide range of antibiofilm agents (e.g., peptides, bacteriophages, nanoparticles, quorum sensing disrupting agents, etc.) and substrates (e.g., metal, plastic, ceramic) showing an exceptional flexibility. An alternative vision of the biofilm challenge can be inspired by the dual nature of biofilms, addressed as “good” or “bad” depending on the specific application. Indeed, biofilm have a great potential in closed systems as small space habitat (e.g., ISS) that can be inspired by their role in “large space habitat” as planet Earth itself. The replication of such a complex biological equilibrium is an open challenge.

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Section: "Promising Coating" Technologies: Nanoparticle and Peptidesmentioning

confidence: 99%

Biofilm contamination in confined space stations: reduction, coexistence or an opportunity?

Marra,

Ferraro,

Caserta

2024

Front. Mater.

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“…Many different methods are used to test for new antibiofilm agents. These are classified as direct and indirect methods such as microscopy (light microscopy, FISH, confocal microscopy, TEM, SEM, AFM), the roll plate method, mass spectrometry, QCM-based method, impedance-based method, Raman spectroscopy, Congo Red agar method, crystal violet method in plates/tubes, PCR, and nuclease-based method (Kırmusaoğlu 2019 ; Mozioğlu and Kocagöz 2020 ; Goudarzi et al 2021 ; Slade et al 2022 ; Kamimura et al 2022 ; Salazar et al 2023 ; Kumar et al 2023 ). Some of them (such as PCR, SEM, TEM, mass spectrometry, AFM, Raman, QCM and impedance-based methods) require sophisticated expensive instrumentation and are not suitable for high throughput studies, especially for the study of multiple antibiofilm agents at different concentrations, while others (crystal violet and Congo Red agar methods) except nuclease-based method (Mozioğlu and Kocagöz 2020 ) are easy to perform but semi-quantitative methods (Slobbe et al 2009 ).…”

Section: Introductionmentioning

confidence: 99%

New-generation biofilm effective antimicrobial peptides and a real-time anti-biofilm activity assay: CoMIC

Polat,

Soyhan,

Cebeci

et al. 2024

Appl Microbiol Biotechnol

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Nowadays, it is very important to produce new-generation drugs with antimicrobial properties that will target biofilm-induced infections. The first target for combating these microorganisms, which are the source itself. Antimicrobial peptides, which are more effective than antibiotics due to their ability to kill microorganisms and use a different metabolic pathway, are among the new options today. The aim of this study is to develop new-generation antibiotics that inhibit both biofilm-producing bacteria and the biofilm itself. For this purpose, we designed four different peptides by combining two amino acid forms (D- and L-) with the same sequence having alpha helix structures. It was found that the combined use of these two forms can increase antimicrobial efficacy more than 30-fold. These results are supported by molecular modeling and scanning electron microscopy (SEM), at the same time cytotoxicity (IC50) and hemotoxicity (HC50) values remained within the safe range. Furthermore, antibiofilm activities of these peptides were investigated. Since the existing biofilm inhibition methods in the literature do not technically simulate the exact situation, in this study, we have developed a real-time observable biofilm model and a new detection method based on it, which we call the CoMIC method. Findings have shown that the NET1 peptide with D-leucine amino acid in its structure and the NET3 peptide with D-arginine amino acid in its structure are effective in inhibiting biofilm. As a conclusion, our peptides can be considered as potential next-generation broad-spectrum antibiotic molecule/drug candidates that might be used in biofilm and clinical important bacteria. Key points • Antimicrobial peptides were developed to inhibit both biofilms producing bacteria and the biofilm itself. • CoMIC will fill a very crucial gap in understanding biofilms and conducting the necessary quantitative studies. • Molecular modelling studies, NET1 peptide molecules tends to move towards and adhere to the membrane within nanoseconds.

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“…It is also possible to employ scanning electron microscopy (SEM), transmission electron microscopy (TEM), cryo-SEM, environmental scanning electron microscopy (ESEM), focused ion beam (FIB)-SEM, atmospheric SEM (ASEM), and super-resolution microscopy (SMR) [4,34,35]. To evaluate the mechanical and physical properties of biofilms, atomic force microscopy (AFM), AFM single-cell force spectroscopy (SCFS), quartz crystal microbalance (QCM), and rheometry are used [46][47][48].…”

Section: Introductionmentioning

confidence: 99%

Monitoring Growth and Removal of Pseudomonas Biofilms on Cellulose-Based Fabrics

et al. 2023

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Biofilms are often tolerant towards routine cleaning and disinfection processes. As they can grow on fabrics in household or healthcare settings, resulting in odors and serious health problems, it is necessary to contain biofilms through eradication strategies. The current study proposes a novel test model for the growth and removal of biofilms on textiles with Pseudomonas fluorescens and the opportunistic nosocomial pathogen Pseudomonas aeruginosa as model organisms. To assess the biofilm removal on fabrics, (1) a detergent-based, (2) enzyme-based, and (3) combined formulation of both detergent and enzymes (F1/2) were applied. Biofilms were analyzed microscopically (FE-SEM, SEM, 3D laser scanning- and epifluorescence microscopy), via a quartz crystal microbalance with mass dissipation monitoring (QCM-D) as well as plate counting of colonies. This study indicated that Pseudomonas spp. form robust biofilms on woven cellulose that can be efficiently removed via F1/2, proven by a significant reduction (p < 0.001) of viable bacteria in biofilms. Moreover, microscopic analysis indicated a disruption and almost complete removal of the biofilms after F1/2 treatment. QCM-D measurements further confirmed a maximal mass dissipation change after applying F1/2. The combination strategy applying both enzymes and detergent is a promising antibiofilm approach to remove bacteria from fabrics.

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Real-Time Detection of the Bacterial Biofilm Formation Stages Using QCM-Based Sensors

Cited by 8 publications

References 35 publications

Biofilm contamination in confined space stations: reduction, coexistence or an opportunity?

Biofilm contamination in confined space stations: reduction, coexistence or an opportunity?

New-generation biofilm effective antimicrobial peptides and a real-time anti-biofilm activity assay: CoMIC

Monitoring Growth and Removal of Pseudomonas Biofilms on Cellulose-Based Fabrics

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