Real-time determination of sarcomere length of a single cardiomyocyte during contraction

Peterson, Pearu; Kalda, Mari; Vendelin, Marko

doi:10.1152/ajpcell.00032.2012

Cited by 24 publications

(24 citation statements)

References 23 publications

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“…4 E), indicating that substantial actomyosin-based force was generated upon shortening. With an increase in frequency, maximal SL decreased and minimal SL increased, both in a linear isometric condition by using carbon fibers (Peterson et al, 2013).…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 95%

Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs

Shintani

Oyama

Kobirumaki-Shimozawa

et al. 2014

Journal of General Physiology

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Section: R E S U L T S a N D Discussionmentioning

confidence: 95%

Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs

Shintani

Oyama

Kobirumaki-Shimozawa

et al. 2014

Journal of General Physiology

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“…Frequency analysis methods have been applied previously for the measurement of sarcomere length using microscopy in the excised rodent heart (3, 4) and in isolated cardiac myocytes (21) as well as in skeletal muscle (22). There are multiple challenges in measuring sarcomere spacing in the beating heart with microscopy.…”

Section: Discussionmentioning

confidence: 99%

“…S7) considering prior work and in analogy to the typical algorithms used in commercially available myocyte imaging systems for in vitro preparations (4,23,24). Previous efforts to optimize algorithms for sarcomere length estimation in isolated cardiomyocytes can also be considered for intravital microscopy (21).…”

Section: Discussionmentioning

confidence: 99%

Intravital imaging of cardiac function at the single-cell level

Aguirre

Vinegoni

Sebas

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Knowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level.intravital micoscopy | molecular imaging | cardiovascular imaging | fluorescence | pacing W ith knowledge of the molecular bases for cardiovascular diseases expanding rapidly, a considerable void exists in our ability to phenotype heart function at the subcellular scale in vivo and in real time. Understanding how the fundamental unit of myocardial function, the cardiomyocyte, responds, adapts, and ultimately fails in response to stress, both individually and in a network of cells contributing to whole-organ function, is central to unraveling mechanisms of disease and designing novel therapies aimed at molecular pathways. The ability to measure single cardiomyocyte contractile function in vivo in the native environment is not possible using existing techniques.Optical microscopy has potential to assess cardiomyocyte structure and function in rodent models (1). Intravital confocal and two-photon microscopy have been used in combination with fluorescence molecular imaging probes in cancer research, immunology, and the neurosciences to reveal biological processes at the cellular level in living organisms (2). Application of intravital techniques for imaging the beating heart in rodent models has been significantly limited by motion from cardiac contraction and respiration, and most studies as a result have used noncontracting Langendorf heart preparations (3-10) or transplanted heart models (11). These model systems do not allow investigation of cardiomyocyte biology in the native heart under physiologic conditions. A few studies have achieved intravital imaging in orthotopic hearts at relatively modest spatial and temporal resolutions that prevent visualization of subcellular structures. Essential to these techniques are methods of macrostabilization such as affixing the heart with sutures (12), compressing the heart with a coverslip (11, 13), bonding the heart with a mechanical stabilizer (14), or suction-based devices (15,16). Although tissue stabilization methods alone can facilitate very-low-resolution cardiac imaging (e.g., microvasculature, cellular recruitment, and flow), they do not achieve the necessary temporal or spatial resolution for subcellular imaging of cardiomyocytes throughout the cardiac cycle. To f...

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“…The pitch glide is numerically estimated using a method introduced by Peterson et al in [19], where it is also shown that this approach is more accurate and faster compared to other methods that are based on the autocorrelation function (ACF) or the fast Fourier transform algorithm (FFT). Following is a short overview of the method.…”

Section: Estimation Of Fundamental Frequencymentioning

confidence: 99%

Pitch glide effect induced by a nonlinear string–barrier interaction

Kartofelev

Stulov

Välimäki

2015

AIP Conference Proceedings

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Interactions of a vibrating string with its supports and other spatially distributed barriers play a significant role in the physics of many stringed musical instruments. It is well known that the tone of the string vibrations is determined by the string supports, and that the boundary conditions of the string termination may cause a short-lasting initial fundamental frequency shifting. Generally, this phenomenon is associated with the nonlinear modulation of the stiff string tension. The aim of this paper is to study the initial frequency glide phenomenon that is induced only by the string-barrier interaction, apart from other possible physical causes, and without the interfering effects of dissipation and dispersion. From a numerical simulation perspective, this highly nonlinear problem may present various difficulties, not the least of which is the risk of numerical instability. We propose a numerically stable and a purely kinematic model of the string-barrier interaction, which is based on the travelling wave solution of the ideal string vibration. The model is capable of reproducing the motion of the vibrating string exhibiting the initial fundamental frequency glide, which is caused solely by the complex nonlinear interaction of the string with its termination. The results presented in this paper can expand our knowledge and understanding of the timbre evolution and the physical principles of sound generation of numerous stringed instruments, such as lutes called the tambura, sitar and biwa. xxx

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Real-time determination of sarcomere length of a single cardiomyocyte during contraction

Cited by 24 publications

References 23 publications

Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs

Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs

Intravital imaging of cardiac function at the single-cell level

Pitch glide effect induced by a nonlinear string–barrier interaction

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