High-throughput
microscopic imaging is highly desirable in biomedical
applications. Advances in computational microscopy have achieved high
space-bandwidth products and even permitted gigapixel imaging in a
stepwise fashion, yet temporal resolution remains challenging for
investigating live-sample dynamics. Here, we report multibeam array
interferometric microscopy (MAIM) for a single-shot high space-bandwidth
product. The MAIM method overcomes the limitations of conventional
digital holographic microscopy, providing complex field reconstruction
with a maximum 5-fold field of view (FOV) increase in a single camera
acquisition, while maintaining sub-nanometer optical path-length stability.
This is achieved by integrating common-path holographic microscopy,
multibeam interference technology, and holographic multiplexing technology.
The temporal resolving power of MAIM is significantly higher than
that of computational illumination microscopy. MAIM has major advantages
over previous holographic multiplexing techniques in that it integrates
more wavefronts and offers high temporal stability. The fundamentals
of MAIM are analyzed theoretically. As a demonstration, we build MAIM
prototypes to increase the FOV by factors of 5, 4, and 3, respectively.
We present proof-of-concept MAIM imaging results of both natural and
artificial samples and show biomedical applications such as monitoring
sub-cellular dynamical phenomena in flowing live erythrocytes in vitro and label-free microrefractometry imaging of unstained
cancer tissue slices. MAIM gives rise to (ultra)fast or long-term
(time-lapse) imaging of nanoscale dynamics of unstained live samples in vitro with a high throughput.