Real-time electrical impedance detection of cellular activities of oral cancer cells

Interdigitated microelectrode-based microchip for electrical impedance spectroscopic study of oral cancer cells

“…The next few hours (2-6 h) was cell spreading period, and it seems cells were fully spread by 6 h. And this was followed by the cell proliferation period in further hours, as evidenced by increased cell number and coverage on the IME electrodes. The observation of these cellular activities with time in this study is consistent with the results in our previous studies using the E-plate with different design of microelectrodes in the wells (Arias et al 2010;Yang et al 2010a).…”

“…By culturing OSCC cells on the microelectrode surface, impedance measurements can provide information about the cell behavior/ activities related to cell adhesion on the electrode surface. Our previous study has demonstrated the use of a commercial real-time impedance instrument (RT-CES from ACEA Bioscience, CA) to study the behaviors of oral cancer cells on microelectrodes including cell attachment, proliferation, drug induced cell apoptosis and inhibition of cell apoptosis (Arias et al 2010). We also used the same real-time impedance technique to distinguish oral cancer cells and non-cancer oral epithelial cells by monitoring impedance-based cell index during cell spreading and cell attachment on the microelectrodes (Yang et al 2010a).…”

A novel 96-well multielectrode array based impedimetric monitoring platform for comparative drug efficacy analysis on 2D and 3D brain tumor cultures

“…In summary, our 96-well multielectrode array based measurement system offers a considerably increased throughput in contrast to small scale chips (Haas et al, 2010;Poenick et al, 2014) while providing the same high quality of acquired whole impedance spectra for extended data analysis. This is in contrast to systems that provide single frequency derived virtual values (Arias et al, 2010;Thakur et al, 2012). Moreover, the multielectrode configuration offers multiple data points per well for analysis, which increases reliability…”

An improved sensitive assay for the detection of PSP toxins with neuroblastoma cell-based impedance biosensor

“…Cellbased impedance biosensor is an emerging technology which can realize real-time, noninvasive monitoring of the growth status of in vitro cultured cells (Tiruppathi et al, 1992;Keese et al, 2002). This biosensor has a wide range of applications, such as drug screening and toxicity assessment (Asphahani and Zhang, 2007;Arias et al, 2010;Zhou et al, 2013). Nevertheless, it is rarely used in marine toxins detection, hardly for PSP toxins.…”