Real-time enzymatic degradation of human dentin collagen fibrils exposed to exogenous collagenase: an AFM study in situ

Zheng, Xinyu; Pan, Haihua; Wang, Z.; Chen, H.

doi:10.1111/j.1365-2818.2010.03412.x

Cited by 19 publications

(21 citation statements)

References 39 publications

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“…Specific enzymes, like MMPs, may contribute to this process [Zheng et al, 2011]. Indeed, collagenase activity was found to be enhanced in the group of bulimia patients with erosion.…”

Section: Discussionmentioning

confidence: 99%

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

et al. 2012

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Patients with bulimia nervosa are at high risk for dental erosion. However, not all bulimic patients suffer from erosion, irrespective of the severity of their eating disorder. It is often speculated that differences in the saliva are important, however, little is known about salivary parameters in bulimic patients, particularly directly after vomiting. The aim of the clinical trial was to compare different salivary parameters of subjects suffering from bulimia with those of healthy controls. Twenty-eight subjects participated (14 patients with bulimia nervosa, 7 of them with erosion; 14 matched healthy controls). Resting and stimulated saliva of all participants was analysed as well as saliva collected from bulimic patients directly and 30 min after vomiting. Parameters under investigation were flow rate, pH, buffering capacity and the enzyme activities of proteases in general, collagenase, pepsin, trypsin, amylase, peroxidase, and lysozyme. Regarding flow rate, pH and buffering capacity only small differences were found between groups; buffering capacity directly after vomiting was significantly lower in bulimic subjects with erosion than in subjects without erosion. Differences in enzymatic activities were more pronounced. Activities of proteases, collagenase and pepsin in resting and proteases in stimulated saliva were significantly higher in bulimic participants with erosion than in controls. Peroxidase activity was significantly decreased by regular vomiting. Proteolytic enzymes seem to be relevant for the initiation and progression of dental erosion directly after vomiting, maybe by both hydrolysis of demineralized dentine structures as well as modulation of the pellicle layer.

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“…Specific enzymes, like MMPs, may contribute to this process [Zheng et al, 2011]. Indeed, collagenase activity was found to be enhanced in the group of bulimia patients with erosion.…”

Section: Discussionmentioning

confidence: 99%

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

et al. 2012

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“…Surface topographical characterization is perhaps the most common use of surface probe microscopy techniques (Zheng et al, 2011). Joel Measurements are performed in air.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

A microscopic evaluation of collagen–bilirubin interactions: in vitro surface phenomenon

Nagarajan

Jayakumar

Rao

et al. 2013

Journal of Microscopy

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SummaryThis study is carried out to understand the morphology variations of collagen I matrices influenced by bilirubin. The characteristics of bilirubin interaction with collagen ascertained using various techniques like XRD, CLSM, fluorescence, SEM and AFM. These techniques are used to understand the distribution, expression and colocalization patterns of collagenbilirubin complexes. The present investigation mimic the in vivo mechanisms created during the disorder condition like jaundice. Fluorescence technique elucidates the crucial role played by bilirubin deposition and interaction during collagen organization. Influence of bilirubin during collagen fibrillogenesis and banding patterns are clearly visualize using SEM. As a result, collagen-bilirubin complex provides different reconstructed patterns because of the influence of bilirubin concentration. Selectivity, specificity and spatial organization of collagen-bilirubin are determined through AFM imaging. Consequently, it is observed that the morphology and quantity of the bilirubin binding to collagen varied by the concentrations and the adsorption rate in protein solutions. Microscopic studies of collagen-bilirubin interaction confirms that bilirubin influence the fibrillogenesis and alter the rate of collagen organization depending on the bilirubin concentration. This knowledge helps to develop a novel drug to inhibit the interface point of interaction between collagen and bilirubin.

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“…Collagen is generally the main protein of connective tissues abundantly occurring also in dentin [12]. Some types of collagens even play a role in tooth enamel formation [13].…”

Section: Human Toothmentioning

confidence: 99%

A Novel Approach to Protein Analysis in Hard Tissues

Hynek

Kučková

Koník

et al. 2011

JSA

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This preliminary study is aimed at a new approach in the protein analysis of hard tissues. Analytical methods that have been used for the identification of proteins in bones and teeth always require one critical step -demineralization. This chemical treatment is responsible for loss of proteins and it also negatively influences the possibility of protein quantification. The method of peptide mass mapping described in this paper facilitates a gentle releasing of peptides from the hard tissues without the loss of qualitative and quantitative information. Using this method led to identification of proteins from chicken thighbone, human jawbone and teeth, which proves the feasibility of this method.

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Real-time enzymatic degradation of human dentin collagen fibrils exposed to exogenous collagenase: an AFM study in situ

Cited by 19 publications

References 39 publications

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

Enzyme Activities in the Oral Fluids of Patients Suffering from Bulimia: A Controlled Clinical Trial

A microscopic evaluation of collagen–bilirubin interactions: in vitro surface phenomenon

A Novel Approach to Protein Analysis in Hard Tissues

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