As an attractive renewable energy source, offshore wind plants are becoming increasingly popular for energy production. However, the performance assessment of offshore wind turbine (OWT) structure is a challenging task due to the combined wind-wave loading and difficulties in reproducing such loading conditions in laboratory. Real-time hybrid simulation (RTHS), combining physical testing and numerical simulation in real-time, offers a new venue to study the structural behavior of OWTs. It overcomes the scaling incompatibilities in OWT scaled model testing by replacing the rotor components with an actuation system, driven by an aerodynamic simulation tool running in real-time. In this study, a RTHS framework for monopile OWTs is proposed. A set of sensitivity analyses is carried out to evaluate the feasibility of this RTHS framework and determine possible tolerances on its design. By simulating different scaling laws and possible error contributors (delays and noises) in the proposed framework, the sensitivity of the OWT responses to these parameters are quantified. An example using a National Renewable Energy Lab (NREL) 5-MW reference OWT system at 1:25 scale is simulated in this study to demonstrate the proposed RTHS framework and sensitivity analyses. Three different scaling laws are considered. The sensitivity results show that the delays in the RTHS framework significantly impact the performance on the response evaluation, higher than the impact of noises. The proposed framework and sensitivity analyses presented in this study provides important information for future implementation and further development of the RTHS technology for similar marine structures.