Real‐time imaging of the lymphatic channels and sentinel lymph nodes of the stomach using contrast‐enhanced ultrasonography with Sonazoid in a porcine model

Kawai, Yoshiko; Ajima, Kumiko; Nagai, Takashi; Kaidoh, Maki; Ohhashi, Toshio

doi:10.1111/j.1349-7006.2011.02048.x

Cited by 18 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some effort has focused upon the use of microbubbles to image lymphatics in the in the stomach and in peritumoral regions of cancer 65, 66. Thus, lymphatic imaging could be amenable to PCN studies as well, and may be aided by the small particle size compared to microbubble agents.…”

Section: Lymphatic Imagingmentioning

confidence: 99%

Phase-Change Nanoparticles Using Highly Volatile Perfluorocarbons: Toward a Platform for Extravascular Ultrasound Imaging

Sheeran²,

et al. 2012

View full text Add to dashboard Cite

Recent efforts using perfluorocarbon (PFC) nanoparticles in conjunction with acoustic droplet vaporization has introduced the possibility of expanding the diagnostic and therapeutic capability of ultrasound contrast agents to beyond the vascular space. Our laboratories have developed phase-change nanoparticles (PCNs) from the highly volatile PFCs decafluorobutane (DFB, bp =-2 °C) and octafluoropropane (OFP, bp =-37 °C ) for acoustic droplet vaporization. Studies with commonly used clinical ultrasound scanners have demonstrated the ability to vaporize PCN emulsions with frequencies and mechanical indices that may significantly decrease tissue bioeffects. In addition, these contrast agents can be formulated to be stable at physiological temperatures and the perfluorocarbons can be mixed to modulate the balance between sensitivity to ultrasound and general stability. We herein discuss our recent efforts to develop finely-tuned diagnostic/molecular imaging agents for tissue interrogation. We discuss studies currently under investigation as well as potential diagnostic and therapeutic paradigms that may emerge as a result of formulating PCNs with low boiling point PFCs.

show abstract

Section: Lymphatic Imagingmentioning

confidence: 99%

Phase-Change Nanoparticles Using Highly Volatile Perfluorocarbons: Toward a Platform for Extravascular Ultrasound Imaging

Sheeran²,

et al. 2012

View full text Add to dashboard Cite

show abstract

“…We have considered the SLN to be constantly subjected to a high lymph flow rate. We reached the conclusion in the above-mentioned ultrasound study [28] in which a high lymph flow rate was detected within the SLN of the stomach using the CEUS-guided method with Sonazoid. Therefore, we investigated the effects of shear stress stimulation on the expression of adhesion molecules on human LEC isolated from the afferent lymphatics nearest the SLN of breast cancers, and on the release of shear stress-mediated ATP from human LEC.…”

Section: (Iii)mentioning

confidence: 85%

“…Contrast imaging using the intragastric or transcutaneous CEUS-guided method with Sonazoid enabled us to produce clear images of the afferent lymph vessel and SLN of the stomach until 2hr after the injection of Sonazoid. Intranodal flow of the microbubble agent could be also clearly identified using tissue linear harmonic images of the SLN [28]. However, it remains unclear which factors play pivotal roles in deciding which lymph node becomes the SLN.…”

Section: (Iii)mentioning

confidence: 94%

New Lymphology Combined with Lymphatic Physiology, Innate Immunology, and Oncology

Ohhashi¹

2014

J Blood Lymph

View full text Add to dashboard Cite

New Lymphology Combined with Lymphatic Physiology, Innate Immunology and OncologyIn more than 85 year-old history of the Gordon Research Conference, a conference on the scheme of lymphatic system was held for the first time on March 2004, in Ventura, California, USA. Such trends of lymphatic Gordon conference are considered to reflect rapid progress of lymphology inspired by the discovery of new markers of lymphatic endothelial cells such as LYVE-1, podoplanin, and Prox1, and then the development of the VEGF C/D and VEGF R3-mediated lymphangiogenesis and lymphatic metastasis of carcinoma cells.As one of lymphatic functions, it is well known that transport and drainage of the substances through the lymph systems play pivotal roles in keeping to homeostasis of internal environment between the cells in tissues in collaboration with the exchange of substances through blood capillaries and venules, A large number of achievements of the classical lymphology have been mainly accumulated in anatomy [1]. While the functions of lymphatic system have been studied by the many investigators of microcirculation, i.e., Courtice FC et al. On the other hand, it is well known that initial signs of main diseases such as inflammation, tumor, and circulatory disorders, appear as functional abnormalities of the internal environment in tissues. When the internal environment is evaluated from the biological defense points of view, these pathophysiological functions are worked by immunological elements with transport and drainage actions of lymphatic system. When the classical lymphology is viewed again from the physiological and pathophysiological significance, the new lymphology may be the royal road of its final goal by the combination with new lymphatic physiology, innate immunology, and oncology. Thus the new obtained physiological findings are summarized as follows:(i) There are marked heterogeneity in lymph dynamic functions, which may be related to gravity and lymph flow volume [7], i.e. heartlike spontaneous contractions are observed physiologically in mesenteric and lower extremities' collecting lymph vessels, but not in facial and neck ones with lymphatic valves [8] (ii) The lymph vessels work physiologically under the circumstance of lower oxygen tension such as 30-35 mmHg [7,9]. Thus oxygen radicals such as nitric oxide (NO) and prostaglandins play as main regulators for controlling the functions of lymphatic endothelial cells and smooth muscles [7,10] (iii)The lymph flow (shear stress stimulation) also play important roles in the production and release of NO [7,11] Taking into consideration of the new findings, we have suggested the concept of new lymphology that new more interesting findings and concept will be easily produced in the field of immunology and oncology in connection with lymphatic physiology if the abovementioned findings were combined with the research of innate immunology and sentinel lymph node concept or lymphatic metastasis concept of carcinoma cells in oncology.In this special commentary, concerning ...

show abstract

“…However, although these techniques have proven to be effective in the identification of SLNs, they both have limitations. Most notably, blue dyes will often stain not only the conjunctiva but also second echelon nodes, as the dyes' small size allows them to pass through the first echelon lymph nodes into multiple nodal levels, which may lead to more extensive dissection . Radiocolloids' small particles can similarly pass through the first echelon lymph nodes .…”

Section: Introductionmentioning

confidence: 99%

“…Although useful in breast and liver tumors and in the oral cavity, this UCA's ability to detect SLNs when injected into the ocular conjunctiva is unknown . Previous studies have shown that Sonazoid is trapped in first‐echelon lymph nodes (i.e., SLNs) and is safe in humans for intravenous injection . Once injected, it is phagocytized by normal lymph node macrophages.…”

Section: Introductionmentioning

confidence: 99%

Contrast‐enhanced ultrasound‐guided Sentinel lymph node biopsy of the ocular conjunctiva

et al. 2014

View full text Add to dashboard Cite

show abstract

Real‐time imaging of the lymphatic channels and sentinel lymph nodes of the stomach using contrast‐enhanced ultrasonography with Sonazoid in a porcine model

Cited by 18 publications

References 30 publications

Phase-Change Nanoparticles Using Highly Volatile Perfluorocarbons: Toward a Platform for Extravascular Ultrasound Imaging

Phase-Change Nanoparticles Using Highly Volatile Perfluorocarbons: Toward a Platform for Extravascular Ultrasound Imaging

New Lymphology Combined with Lymphatic Physiology, Innate Immunology, and Oncology

Contrast‐enhanced ultrasound‐guided Sentinel lymph node biopsy of the ocular conjunctiva

Contact Info

Product

Resources

About