Real-time impedance analysis of host cell response to meningococcal infection

Slanina, Heiko; König, Alexandra; Claus, Heike; Frosch, Matthias; Schubert‐Unkmeir, Alexandra

doi:10.1016/j.mimet.2010.11.004

Cited by 47 publications

(28 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…xCELLigence system -Real-Time Cell Analyser (RTCA) The RTCA system was described by many authors (Xing et al 2006;Ceriotti et al 2007;Slanina et al 2011;Garcia et al 2013). It utilizes a series of micro wells with bottoms covered by 80% with microelectrodes that measure cell viability by monitoring cell proliferation and morphology.…”

Section: Chemicalsmentioning

confidence: 99%

Real-time monitoring of cadmium toxicity in rabbit kidney cells

et al. 2015

View full text Add to dashboard Cite

The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and proliferative activity during the whole time of experiment. Moreover, after 24 h exposure of cells to cadmium, colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyl tetrazolium bromide (MTT) test was used to measure the metabolic activity and cytotoxicity was determined by measurement of lactate dehydrogenase (LDH) leaked from damaged cells. We found that renal cells exposed to lower concentrations (5-10 mg·l -1 ) of cadmium tend to grow similarly to control cells, however, cell index was significantly different (P < 0.05) after 24 h. With increasing concentration of cadmium (15-50 mg·l -1 ) significantly lower proliferative (P < 0.05) and metabolic activity (P < 0.05) of cells was observed and cytotoxicity increased simultaneously (P < 0.001). In addition, we found that the real-time monitoring of the cell response was significantly correlated with commonly used biological methods for toxicity measurement, for MTT assay R 2 was 0.9448 (P < 0.01) and for LDH assay R 2 was 0.9466 (P < 0.01), respectively. The present study is the first report when combination of RTCA, MTT assay and LDH test was used for cadmium nephrotoxicity assessment. In all these methods, the toxic effect of cadmium on rabbit kidney cells increased in a concentration-dependent manner. xCELLigence system, nephrotoxicity, cytotoxicity, lactate dehydrogenaseIncreased industrial activities brought gradual redistribution of a number of toxic elements from the terrestrial crust into the environment and thus increased the potential exposure of humans and animals. With rising environmental pollution the interest arises in the consequences of the action of xenobiotics, including hazardous chemical elements, on live organisms (Nováková et al. 2007;Čelechovská et al. 2008). A series of trials were designed to evaluate the responses of tissues and organs of animal species of economic importance, such as the rabbit, to trace elements (cadmium, lead, mercury) at concentrations that could potentially exist in the feeds on a farm. The daily rations included carrots, potatoes, or beetroot. Cadmium belongs to heavy metals widely distributed in the environment, obtained as a by-product of refining. Its compounds are used in the electroplating of metals, alkaline batteries or in compounds with other metals. Relatively high quantities of cadmium are present in phosphate fertilisers (from some locations), which increase the concentration of cadmium in soil and plants. Cadmium is also highly toxic to organisms living in the aqueous environment (Koréneková et al. 2002). Because of its ability to reabsorb and accumulate divalent metals, kidney is the first target organ of heavy metal toxicity. The extent of renal da...

show abstract

Section: Chemicalsmentioning

confidence: 99%

Real-time monitoring of cadmium toxicity in rabbit kidney cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The RTCA assay has been applied in a number of cell-based assays. These include cytotoxicity, cell adhesion and spreading, cell proliferation, cell invasion, cell migration, cell apoptosis, RNA interference, functional monitoring of receptormediated signaling, stem cell-derived cardiomyocyte beating, bacterial toxin detection, analyzing bacterial and host cell interaction, virus-induced cytopathic effect (CPE) quantification, neutralizing antibody detection, parasite infective activity assessment, as well as functional detection and monitoring of host immune system [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43].…”

Section: Abstract: Clostridium Difficile • Detection • Quantificationmentioning

confidence: 99%

Real-time cellular analysis for quantitative detection of functionalClostridium difficiletoxin in stool

Huang

Jin³

et al. 2014

Expert Review of Molecular Diagnostics

View full text Add to dashboard Cite

Rapid and accurate diagnosis and monitoring of Clostridium difficile infection (CDI) is critical for patient care and infection control. We will briefly review current laboratory techniques for the diagnosis of CDI and identify aspects needing improvement. We will also introduce a real-time cellular analysis (RTCA) assay developed for the diagnosis and monitoring of CDI using electronic impedance to assess the cell status. The RTCA assay uses impedance measurement to detect minute physiological changes in cells cultured on gold microelectrodes embedded in glass substrates in the bottom of microtiter wells. This assay has been adapted for quantitative detection of C. difficile functional toxin directly from stool specimens. Compared to conventional techniques and molecular assays, the RTCA assay provides a valuable tool for the diagnosis of CDI as well as for the assessment of clinical severity and for monitoring therapeutic efficacies.

show abstract

“…CPE is a basis for studies using cell cultures. A novel method for the real-time assay of in vitro cells is real-time cell analysis (RTCA) (4,5,9,10,11,14), also applied to diagnosis of viral diseases (2,3,12,13), and it creates a possibility for improvement in that area. The aim of this experiment was to evaluate the applicability of RTCA in CPE detection in SVDV-infected cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

Paprocka

Kęsy

2015

Bulletin of the Veterinary Institute in Pulawy

View full text Add to dashboard Cite

A swine vesicular disease virus (SVDV) replication assay in IB-RS-2, SK-6, and PK-15 cell cultures was performed using the xCELLigence system. The cell status was monitored by impedance measurement, expressed as cell index (CI). Proliferation of particular cells was examined at the beginning of the study. The cells exhibited the ability to form a monolayer, and the CI values increased with the cell culture growth. After about 23 h and while still in the growth phase, the cells were infected with decimal virus dilutions (10 -1 -10 -6 ) containing from 100 000 to 1 median tissue culture infectious doses (TCID50). SVDV replication in cell cultures induced a change in cell index; together with the occurrence of cytopathic effect (CPE), the CI values declined. A significant correlation between the concentration of the virus used and CPE occurrence was found. The results also enabled determination of cell sensitivity to SVDV infection. The highest sensitivity was exhibited by IB-RS-2, followed by SK-6. To conclude, the xCELLigence System was used effectively and evaluated as being an efficient tool for CPE detection and SVDV replication analysis in cell cultures. Compared to the standard method , it enabled a more precise assessment of viral replication based on the quantitative CI measurement, providing additional current information.

show abstract

Real-time impedance analysis of host cell response to meningococcal infection

Cited by 47 publications

References 33 publications

Real-time monitoring of cadmium toxicity in rabbit kidney cells

Real-time monitoring of cadmium toxicity in rabbit kidney cells

Real-time cellular analysis for quantitative detection of functionalClostridium difficiletoxin in stool

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

Contact Info

Product

Resources

About