2014
DOI: 10.1038/srep06384
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Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking

Abstract: We observed the dynamic three-dimensional (3D) single molecule behaviour of acetylcholine-binding protein (AChBP) and nicotinic acetylcholine receptor (nAChR) using a single molecule tracking technique, diffracted X-ray tracking (DXT) with atomic scale and 100 μs time resolution. We found that the combined tilting and twisting motions of the proteins were enhanced upon acetylcholine (ACh) binding. We present the internal motion maps of AChBP and nAChR in the presence of either ACh or α-bungarotoxin (αBtx), wit… Show more

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Cited by 40 publications
(56 citation statements)
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“…In this study, we developed a novel method for dynamic observation of local supersaturated solutions at microsecond time scales using an X-ray single molecule methodology, also known as diffracted X-ray tracking (DXT) 15 16 17 . In particular, DXT can be used to detect high accuracy rotational angular observations in solution systems.…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…In this study, we developed a novel method for dynamic observation of local supersaturated solutions at microsecond time scales using an X-ray single molecule methodology, also known as diffracted X-ray tracking (DXT) 15 16 17 . In particular, DXT can be used to detect high accuracy rotational angular observations in solution systems.…”
mentioning
confidence: 99%
“…We utilized DXT as a high time resolution (μs) and high positional accuracy (pm) 17 X-ray single molecule observation method to investigate the properties of supersaturated solutions ( Fig. 1a ).…”
mentioning
confidence: 99%
“…However, previous attempts of AChBP expression in E . coli resulted in accumulation of these proteins in inclusion bodies [ 38 ]. This necessitated the use of expensive and time-consuming re-solubilization techniques to recover the expressed protein.…”
Section: Discussionmentioning
confidence: 99%
“…Previous attempts to express the AChBPs in E . coli produced only insoluble AChBP, requiring expensive and time-consuming stabilization and refolding steps to obtain functional protein [ 38 ].…”
Section: Introductionmentioning
confidence: 99%
“…This diffracted X-ray tracking 5 7 (DXT) method can trace rotational motions within single protein molecules using X-rays with a wide energy bandwidth. Among various applications, DXT has been utilised to monitor the dynamic twisting motions of membrane proteins 8 , 9 chaperonin proteins 10 , 11 or other globular macromolecules 12 14 . This technique is precise to a sub-mrad angular scale.…”
Section: Introductionmentioning
confidence: 99%