Real-Time Light Scattering Tracking of Gold Nanoparticles- bioconjugated Respiratory Syncytial Virus Infecting HEp-2 Cells

Abstract

Real-time tracking of virus invasion is crucial for understanding viral infection mechanism, which, however, needs simple and efficient labeling chemistry with improved signal-to-noise ratio. For that purpose, herein we investigated the invasion dynamics of respiratory syncytial virus (RSV) through dark-field microscopic imaging (iDFM) technique by using Au nanoparticles (AuNPs) as light scattering labels. RSV, a ubiquitous, non-segmented, pleiomorphic and negative-sense RNA virus, is an important human pathogen in infants, the elderly, and the immunocompromised. In order to label the enveloped virus of paramyxoviridae family, an efficient streptavidin (SA)-biotin binding chemistry was employed, wherein AuNPs and RSV particles modified with SA and biotin, respectively, allowing the AuNP-modified RSVs to maintain their virulence without affecting the native activities of RSV, making the long dynamic visualization successful for the RSV infections into human epidermis larynx carcinoma cells.

Cellular uptake of nanoparticles: journey inside the cell

“…10g demonstrates the utility of this technique in monitoring respiratory syncytial virus (RSV) infection in larynx epidermal cells using virus surface-labeled Au NPs. 225 The authors tracked RSV cell-entry kinetics and demonstrated that 13-nm Au NPs probes can serve as non-interfering, non-photobleaching dark-field imaging probes.…”

“…i) Laser-ablation inductively coupled plasma mass spectrometry (ICP-MS) imaging (heatmap) of mouse fibroblasts (grayscale) incubated with Au (left) and Ag (right) nanoparticles. Reproduced with permission from (a) 207 , (b) 209 , (c) 243 , (d) 214 , (e) 217 , (f) 221 , (g) 225 , (h) 228 , and (i) 234 . Copyright (a) 2016 American Chemical Society, (b) 2014 Købler et al ., (c) 2015 Grant et al ., (d) 2014 Nature Publishing Group, (e) 2013 Nature Publishing Group, (f) 2014 Nature Publishing Group, (g) 2014 Nature Publishing Group, (h) 2016 Strohm et al ., and (i) 2012 American Chemical Society.…”

Positron emission tomography and nanotechnology: A dynamic duo for cancer theranostics

“…Owing to their unique physicochemical properties, varied and tailorable shapes, sizes and chemical compositions, gold nanostructures make excellent candidates for molecular imaging and, potentially, therapy of various diseases, including cancer [155, 156], cardiovascular disease [157–159], viral infections [160–162], and others. [163, 164] Gold nanoparticles (AuNPs) display excellent biocompatibility as gold is relatively inert in biological environments.…”

Femtomolar oligonucleotide detection by a one-step gold nanoparticle-based assay

“…However, for typical applications where sub-picomolar detection is sufficient, the simplicity of direct oligonucleotide quantification assays makes them more attractive for various applications. [29][30][31][32][33][34][35][36][37] including SNP detection [38][39][40][41], as well as for gene delivery [42] and molecular imaging [43,44]. In applications typical for oligonucleotide sensing the analyte induces the agglomeration of AuNPs by hybridization, resulting in a red shift of the nanoparticle plasmon peak [45].…”