Real Time Measurement of Dopamine Releasing under Optogenetic Stimulation

Abstract

Abstract-Dopamine (DA) is a neurotransmitter involved in controlling of fine movement, which is deficient in Parkinson's disease (PD). Measurement of DA is an urgent need concerning the development of PD therapy. We've developed a fastscan cyclic voltammetry (FSCV) for real time measurement of dopamine releasing in dopaminergic SH-SY5Y cells under tissue-specific optogenetic stimulation. With the novel cellular optical-induced DA-releasing model, the performance of FSCV will be validated under various stimulating parameters.