2020
DOI: 10.1101/2020.06.29.177675
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Real-Time Measurement of Stimulated Dopamine Release in Compartments of the AdultDrosophila melanogasterMushroom Body

Abstract: AbstractDrosophila melanogaster, the fruit fly, is an exquisite model organism to understand neurotransmission. Dopaminergic signaling in the Drosophila mushroom body (MB) is involved in olfactory learning and memory, with different compartments controlling aversive learning (corner) vs appetitive learning (medial tip). Here, the goal was to develop techniques to measure endogenous dopamine in compartments of the MB for the … Show more

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Cited by 6 publications
(8 citation statements)
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“…The Daws group also found serotonin reuptake did not significantly change in SERT KO mice (Bowman et al, 2020), which implies compensation by other neurotransmitter systems. Our group has adapted FSCV to measure neurotransmitter dynamics in Drosophila (Borue et al, 2009; Shin et al, 2018; Shin et al, 2020; Vickrey et al, 2009). Serotonin release is stimulated using optogenetics, as the UAS/Gal4 system is used to express CsChrimson with a tryptophan hydroxylase (trh‐Gal4) driver that localizes it in serotonin neurons (Dunham & Venton, 2020; Privman & Venton, 2015).…”
Section: Introductionmentioning
confidence: 99%
“…The Daws group also found serotonin reuptake did not significantly change in SERT KO mice (Bowman et al, 2020), which implies compensation by other neurotransmitter systems. Our group has adapted FSCV to measure neurotransmitter dynamics in Drosophila (Borue et al, 2009; Shin et al, 2018; Shin et al, 2020; Vickrey et al, 2009). Serotonin release is stimulated using optogenetics, as the UAS/Gal4 system is used to express CsChrimson with a tryptophan hydroxylase (trh‐Gal4) driver that localizes it in serotonin neurons (Dunham & Venton, 2020; Privman & Venton, 2015).…”
Section: Introductionmentioning
confidence: 99%
“…Ultimately, this study shows that SERT inhibition is not required to achieve behavioral effects with microdoses of ketamine and that ketamine may increase feeding behavior via NMDA receptor antagonism, while locomotion behavior may be caused by 5‐HT 1A or 5‐HT 2 agonism (Kraus et al., 2019; Spies et al., 2018). Future studies in genetically altered flies, where NMDA or serotonin receptors are over‐ or under‐expressed, would help confirm these are the targets of ketamine or other SSRIs to elicit behavioral changes (Shin et al., 2020; Silva et al., 2014). Future work should also investigate real‐time changes with ketamine or SSRIs on other neurotransmitters, such as glutamate (Marvin et al., 2018; Richter et al., 2018).…”
Section: Discussionmentioning
confidence: 99%
“…This study shows that SERT inhibition is not required to achieve behavioral effects with microdoses of ketamine, and that ketamine may increase feeding behavior via NMDA receptor antagonism, while locomotion behavior may be caused by ketamine acting as a 5-HT 1A or 5-HT 2 agonist (Kraus et al, 2019; Spies et al, 2018). Future studies in genetically altered flies, where NMDA or serotonin receptors are knocked-down, would help confirm these are the targets of ketamine or other SSRIs to elicit behavioral effects (Shin et al, 2020; Silva et al, 2014). Future work should also investigate the real-time changes of microdoses of ketamine or SSRIs on other neurotransmitters, such as glutamate (Marvin et al, 2018; Richter et al, 2018).…”
Section: Discussionmentioning
confidence: 99%