Real-time method for determining the colony-forming cell content of human hematopoietic cell cultures

Collins, Paul C.; Nielsen, Lars K.; Wong, Carmen; Papoutsakis, E. T.; Miller, William M.

doi:10.1002/(sici)1097-0290(19970820)55:4<693::aid-bit11>3.0.co;2-d

Cited by 25 publications

(28 citation statements)

References 16 publications

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“…Lactate production rate was used as the indicator of metabolic activity, because the glucose consumption rate is more variable, especially in early stages of culture (27). Hematopoietic cells from CB MNC were quiescent initially and very low metabolic activity was observed (Fig.…”

Section: Effects Of Surface Hydrolysis Of Pet Polymer Filmmentioning

confidence: 99%

“…2C). This indicated that progenitors had higher metabolic rate compared to mature blood cells (17,27). The higher CD34 1 cell frequency also resulted in higher total cell number due to higher potential of CD34 1 cells to produce mature blood cells (Fig.…”

Section: Effects Of Surface Hydrolysis Of Pet Polymer Filmmentioning

confidence: 99%

“…For example, our group has demonstrated that trophoblast-like ED 27 cells can maintain in a proliferative state or be driven toward differentiation by altering the physical properties of nonwoven polymer matrices (18)(19)(20). Nonwoven matrices have also been employed as 3-D scaffolds for engineering porcine muscular artery and canine urinary bladder (21,22).…”

Section: Introductionmentioning

confidence: 99%

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Human Cord Cell Hematopoiesis in Three-Dimensional Nonwoven Fibrous Matrices: In Vitro Simulation of the Marrow Microenvironment

Kniss

et al. 2001

Journal of Hematotherapy & Stem Cell Research

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Current hematopoietic culture systems mainly utilize two-dimensional devices with limited ability to promote self-renewal of early progenitors. In vivo-like three-dimensional (3-D) culture environments might be conducive to regulating stem cell proliferation and differentiation similar to in vivo hematopoiesis. The few 3-D cultures reported in the literature either produced few progenitors or provided little information about microenvironment. In this study, we constructed a 3-D hematopoietic microenvironment composed of nonwoven matrix and human cord blood (CB) cells to simulate the marrow microenvironment and expand cord progenitors. Nonwoven polyethylene terephthalate (PET) fabric with defined microstructure was used as the 3-D scaffold and the PET surface was modified by hydrolysis to improve cell adhesion. Different cell organizations were formed in a 3-D matrix in a developmental manner, from individual cells and cells bridging between fibers to large cell aggregates. Both stromal and hematopoietic cells were distributed spatially within the scaffold. Compared to two-dimensional (2-D) CD34(+) cell culture, 3-D culture produced 30-100% higher total cells and progenitors without exogenous cytokines. With thrombopoietin and flt-3/flk-2 ligand, it supported two- to three-fold higher total cell number (62.1- vs. 24.6-fold), CD34(+) cell number (6.8- vs. 2.8-fold) and colony-forming unit (CFU) number for 7-9 weeks (n = 6), indicating a hematopoiesis pathway that promoted progenitor production. Culture in 3-D nonwoven matrices enhanced cell-cell and cell-matrix interactions and allowed 3-D distribution of stromal and hematopoietic cells. The formation of cell aggregates and higher progenitor content indicated that the spatial microenvironment in 3-D culture played an important role in promoting hematopoiesis. This 3-D culture system can be used as an in vitro model to study stem cell or progenitor behavior, and to achieve sustained progenitor expansion.

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Section: Effects Of Surface Hydrolysis Of Pet Polymer Filmmentioning

confidence: 99%

Section: Effects Of Surface Hydrolysis Of Pet Polymer Filmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human Cord Cell Hematopoiesis in Three-Dimensional Nonwoven Fibrous Matrices: In Vitro Simulation of the Marrow Microenvironment

Kniss

et al. 2001

Journal of Hematotherapy & Stem Cell Research

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“…This reinforces the assumption of a reliance on glycolysis during proliferation and maturation, with a switch to OXPHOS during differentiation. Cord blood mononuclear cell cultures which contain high populations of erythroid-burst forming units during proliferation also show a high Y Lac/Gluc [33].…”

Section: Resultsmentioning

confidence: 92%

Metabolic profiling of hematopoietic stem and progenitor cells during proliferation and differentiation into red blood cells

et al. 2016

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“…Collins et al (1997) have shown that the maximum percentage of cells that are CFC (%CFC) coincides with the peaks of specific lactate production rate and glucose consumption rate for cultures of cord blood (CB) mononuclear cells (MNC), peripheral blood (PB) MNC, and PB CD34+ cells carried out in spinner flasks and in T flasks. It has been shown that the correlation between %CFC and specific lactate production rate can be well described by a two-population (CFC and other cells) model for experiments with similar/identical inoculum density, cytokine combination, and culture conditions (Collins et al, 1997). However, the model parameters can vary substantially at different inoculum densities, cytokine combinations, initial %CFC contents, and culture conditions.…”

Section: Introductionmentioning

confidence: 98%

Model-based estimation of myeloid hematopoietic progenitor cells in ex vivo cultures for cell and gene therapies

Yang

Papoutsakis

Miller

2000

Biotechnol. Bioeng.

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Real-time method for determining the colony-forming cell content of human hematopoietic cell cultures

Cited by 25 publications

References 16 publications

Human Cord Cell Hematopoiesis in Three-Dimensional Nonwoven Fibrous Matrices: In Vitro Simulation of the Marrow Microenvironment

Human Cord Cell Hematopoiesis in Three-Dimensional Nonwoven Fibrous Matrices: In Vitro Simulation of the Marrow Microenvironment

Metabolic profiling of hematopoietic stem and progenitor cells during proliferation and differentiation into red blood cells

Model-based estimation of myeloid hematopoietic progenitor cells in ex vivo cultures for cell and gene therapies

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