Real-time monitoring of adhesion and aggregation of platelets using thickness shear mode (TSM) sensor

Ergezen, E.; Appel, Marilyn H.; Shah, Piyush; Kresh, J. Yasha; Lec, R.M.; Wootton, David

doi:10.1016/j.bios.2007.05.009

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…In this system, AFP is adsorbed onto a gold electrode of the TSM sensor and lectin applied. Binding is determined by a change in resonant frequency of the sensor [34]. As this Figure shows, there is over a 6-fold increase in binding of the N224Q lectin as compared to the wild type recombinant lectin or the commercially purchased nAAL in line with the results presented in Figs.…”

Section: Resultssupporting

confidence: 74%

Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans

Romano

Mackay

Vong

et al. 2011

Biochemical and Biophysical Research Communications

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Changes in glycosylation have long been associated with disease. While there are many methods to detect changes in glycosylation, plant derived lectins are often used to determine changes on specific proteins or molecules of interest. One change in glycosylation that has been observed by us and by others is a disease or antigen associated increase in fucosylation on N-linked glycans. To measure this change, the fucose binding Aleuria aurantia lectin (AAL) is often utilized in plate and solution based assays. AAL is a mushroom derived lectin that contains five fucose binding sites that preferentially bind fucose linked (α-1,3, α-1,2, α-,4, and α-1,6) to N-acetyllactosamine related structures. Recently, several reports by us and by others have indicated that specific fucose linkages found on certain serum biomarker glycoprotein's are more associated with disease than others. Taking a site-directed mutagenesis approach, we have created a set of recombinant AAL proteins that display altered binding affinities to different analytes containing various fucose linkages.

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Section: Resultssupporting

confidence: 74%

Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans

Romano

Mackay

Vong

et al. 2011

Biochemical and Biophysical Research Communications

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“…Different types of sulfonated polymers, including polystyrenes with different sulfonation levels, various sulfonated polyethylene oxides, as well as sulfated chitosan were studied with regards to their antigolant activities by observing the binding to factor XII (FXII) (Chen et al ., 2007b) or antithrombin II (Jung et al ., 2009a). Platelet conformational changes (Fatisson et al , 2008) and platelet aggregation (Ergezen et al , 2007) were also studied using QCM and the selective sensitivity of this technique for the monitoring of platelet function and inhibition could be demonstrated (Figure 3). The interaction of thrombocyte vesicles with sensor surfaces of different lipophilicity was examined as a model for understanding the membrane properties of thrombocytes (Agmo Hernandez et al , 2008).…”

Section: Hemostasismentioning

confidence: 99%

“…The obtained results were supported by epifluorescence and complementary SEM studies, and demonstrate the monitor platelet function and inhibition, particularly aggregation. From (Ergezen et al , 2007), reprinted with permission.…”

Section: Hemostasismentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

163

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Since the publication of the original review of piezoelectric acoustic sensors in this series there has been a consistent, gradual expansion in the number of published papers using 'quartz crystal microbalances' (QCM). Between 2001 and 2009, the number of QCM publications per annum has increased from 49 to 273, with a two-fold increase in papers per annum between 2004 and 2008. Within the field, comparing the time covered by the current to the previous review, there are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase) and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterization of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This paper highlights theoretical and practical aspects of the principles that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and membrane interfaces.

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“…In other words, there are fewer equations than the material/geometrical parameters of the interface, therefore, the stochastic method is the only approach that can address this problem mathematically. (Wegener et al, 1999, Ergezen et al, 2007. In contrast, there has been very limited (Lucklum et al, 2001) theoretical study and no experimental studies based on the MTSM sensor for quantitative characterization of multi-layer biological processes.…”

Section: Quantification Of Thickness Shear Mode (Tsm) Sensor Responsementioning

confidence: 99%

“…Phenomena such as cell adhesion (Soonjin et al, 2006. ), superhydrophobicity (Sun et al, 2006, Roach et al, 2007, particle-surface interactions (Zhang et al,2005), organic and inorganic particle manipulation (Desa et al, 2010) and rheological and interfacial properties of blood coagulation (Ergezen et al 2007) were studied using TSM sensors. Due to the high interfacial sensitivity of TSM sensors, it has been shown that cell motility can be monitored by analyzing the noise of the TSM sensor response (Sapper et al, 2006).…”

Section: Introductionmentioning

confidence: 99%