Real-time monitoring of adipocyte differentiation using a capacitance sensor array

Lee, Rimi; Jung, Inji; Park, Mi-Young; Ha, Hunjoo; Yoo, Kyung Hwa

doi:10.1039/c3lc50453k

Cited by 11 publications

(9 citation statements)

References 21 publications

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“…A variation in the cell membrane capacitance between undifferentiated stem cell, osteo-and adipo-induced was also measured and pointed out as an early (less than 4 days) marker of stem-cell fate. Similar changes in dielectric properties accompanying stem-cell differentiation towards adipocytes was also reported by Lee et al [58] and Fu et al [59], which was related to lipid vacuole accumulation. In a similar work, Angstmann et al [56] used two commercial systems, ECIS and xCELLigence, to examine differences in early phases of MSC differentiation towards adipocytes and osteoblasts, while Kramer et al [60] have used xCELLigence to study adipogenesis of the preadipocyte 3T3-L1 cells.…”

Section: Current Progress Of Impedance-based Cellularsupporting

confidence: 85%

Impedance-based cellular assays for regenerative medicine

Gamal

Underwood

et al. 2018

Phil. Trans. R. Soc. B

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Therapies based on regenerative techniques have the potential to radically improve healthcare in the coming years. As a result, there is an emerging need for non-destructive and label-free technologies to assess the quality of engineered tissues and cell-based products prior to their use in the clinic. In parallel, the emerging regenerative medicine industry that aims to produce stem cells and their progeny on a large scale will benefit from moving away from existing destructive biochemical assays towards data-driven automation and control at the industrial scale. Impedance-based cellular assays (IBCA) have emerged as an alternative approach to study stem-cell properties and cumulative studies, reviewed here, have shown their potential to monitor stem-cell renewal, differentiation and maturation. They offer a novel method to non-destructively assess and quality-control stem-cell cultures. In addition, when combined with disease models they provide complementary insights as label-free phenotypic assays. IBCA provide quantitative and very sensitive results that can easily be automated and up-scaled in multi-well format. When facing the emerging challenge of real-time monitoring of three-dimensional cell culture dielectric spectroscopy and electrical impedance tomography represent viable alternatives to two-dimensional impedance sensing.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.

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Section: Current Progress Of Impedance-based Cellularsupporting

confidence: 85%

Impedance-based cellular assays for regenerative medicine

Gamal

Underwood

et al. 2018

Phil. Trans. R. Soc. B

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“…Under proliferation conditions in PM, the capacitance increased steadily due to increases in cell size and number (Supplementary Fig. S4) and became nearly constant after ~4.5 days, as previously reported14151617. When the cells grew under differentiation conditions, the capacitance also increased, but the rate of increase was much slower than that of the cells grown under proliferation conditions (Supplementary Figs.…”

Section: Resultssupporting

confidence: 80%

“…Cells attach and spread on the surface of the sensing electrode and passively block the current, and thus the electrode impedance is affected by the shape, adhesion, and/or mobility of adherent cells1213. However, compared to the electrode impedance, the capacitance (or the dielectric constant) of cells provides more direct information on cellular activities14151617; the fate potential of NSCs was previously reported to be more closely related to cell membrane capacitance than to conductance18. On this study, we have developed a capacitance sensor to monitor the differentiation of hNSCs.…”

mentioning

confidence: 99%

Real-Time Discrimination between Proliferation and Neuronal and Astroglial Differentiation of Human Neural Stem Cells

Lee

Kim

Han

et al. 2014

Sci Rep

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Neural stem cells (NSCs) are characterized by a capacity for self-renewal, differentiation into multiple neural lineages, all of which are considered to be promising components for neural regeneration. However, for cell-replacement therapies, it is essential to monitor the process of in vitro NSC differentiation and identify differentiated cell phenotypes. We report a real-time and label-free method that uses a capacitance sensor array to monitor the differentiation of human fetal brain-derived NSCs (hNSCs) and to identify the fates of differentiated cells. When hNSCs were placed under proliferation or differentiation conditions in five media, proliferating and differentiating hNSCs exhibited different frequency and time dependences of capacitance, indicating that the proliferation and differentiation status of hNSCs may be discriminated in real-time using our capacitance sensor. In addition, comparison between real-time capacitance and time-lapse optical images revealed that neuronal and astroglial differentiation of hNSCs may be identified in real-time without cell labeling.

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“…1 ). In our system, cells were plated between gold electrodes and the change in dielectric constant (ε) is measured to estimate the capacitance ( C ) with an equation C = ε A / d , where A is the electrode area and d is the distance between the two electrodes 12 13 14 15 . To increase paracellular permeability of cerebral endothelial cells, we treated endothelial cells in monolayer with VEGF (20 ng/mL), which is known to increase cerebral vascular permeability, 16 17 after 24 hours of stabilization.…”

Section: Resultsmentioning

confidence: 99%

“…The capacitance (or dielectric constant) could be also a potential candidate to estimate the changes in the integrity of cerebral microvasculature. This method is a useful way to obtain real-time electrical properties such as cell membrane capacitance and cytoplasm conductivity 10 Upon application of AC electrical fields, cells demonstrate specific dielectric responses according to interfacial polarization patterns 11 Interestingly, recent studies have demonstrated that this technique can be utilized to observe various cellular functions including cell viability/death, endocytosis, and differentiation 10 12 13 14 15 In the similar manner, we speculated that we could differentiate the status of paracellular permeability of cerebral microvascular endothelial cell monolayer with the capacitance sensor array.…”

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confidence: 99%

Real-time estimation of paracellular permeability of cerebral endothelial cells by capacitance sensor array

Lee

Kim

et al. 2015

Sci Rep

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Vascular integrity is important in maintaining homeostasis of brain microenvironments. In various brain diseases including Alzheimer’s disease, stroke, and multiple sclerosis, increased paracellular permeability due to breakdown of blood-brain barrier is linked with initiation and progression of pathological conditions. We developed a capacitance sensor array to monitor dielectric responses of cerebral endothelial cell monolayer, which could be utilized to evaluate the integrity of brain microvasculature. Our system measured real-time capacitance values which demonstrated frequency- and time-dependent variations. With the measurement of capacitance at the frequency of 100 Hz, we could differentiate the effects of vascular endothelial growth factor (VEGF), a representative permeability-inducing factor, on endothelial cells and quantitatively analyse the normalized values. Interestingly, we showed differential capacitance values according to the status of endothelial cell monolayer, confluent or sparse, evidencing that the integrity of monolayer was associated with capacitance values. Another notable feature was that we could evaluate the expression of molecules in samples in our system with the reference of real-time capacitance values. We suggest that this dielectric spectroscopy system could be successfully implanted as a novel in vitro assay in the investigation of the roles of paracellular permeability in various brain diseases.

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Real-time monitoring of adipocyte differentiation using a capacitance sensor array

Cited by 11 publications

References 21 publications

Impedance-based cellular assays for regenerative medicine

Impedance-based cellular assays for regenerative medicine

Real-Time Discrimination between Proliferation and Neuronal and Astroglial Differentiation of Human Neural Stem Cells

Real-time estimation of paracellular permeability of cerebral endothelial cells by capacitance sensor array

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