Real time monitoring of biomaterial-mediated inflammatory responses via macrophage-targeting NIR nanoprobes

Zhou, Jun; Tsai, Yi Ting; Hong, Wenting; Baker, David W.; Tang, Liping

doi:10.1016/j.biomaterials.2011.08.064

Cited by 46 publications

(71 citation statements)

References 64 publications

(73 reference statements)

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“…When the different histopathological characteristics in rat subcutaneous tissue are observed at various time intervals after being in contact with the materials tested, they allow a more precise evaluation, because of the dynamism of inflammatory reactions (Zhou et al 2011). In this study, the first analysis was performed after 7 days because, it is only after this period that a more organized inflammatory reaction can be expected (Santos et al 2010(Santos et al , 2014a.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Mesquita

Santos

Sampaio

et al. 2017

An. Acad. Bras. Ciênc.

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The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of resin-modified glass ionomer cements. Sixty male Wistar rats were selected and divided into four groups: Control Group; Crosslink Group; RMO Group and Transbond Group. The materials were inserted into rat subcutaneous tissue. After time intervals of 7, 15 and 30 days morphological analyses were performed. The histological parameters assessed were: inflammatory infiltrate intensity; reaction of multinucleated giant cells; edema; necrosis; granulation reaction; young fibroblasts and collagenization. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn test (P<0.05). After 7 days, Groups RMO and Transbond showed intense inflammatory infiltrate (P=0.004), only Group RMO presented greater expression of multinucleated giant cell reaction (P=0.003) compared with the control group. After the time intervals of 15 and 30 days, there was evidence of light/moderate inflammatory infiltrate, lower level of multinucleated giant cell reaction and thicker areas of young fibroblasts in all the groups. The hypothesis was rejected. The Crosslink cement provided good tissue response, since it demonstrated a lower level of inflammatory infiltrate and higher degree of collagenization, while RMO demonstrated the lowest level of biocompatibility.

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Section: Discussionmentioning

confidence: 99%

“…Nevertheless, although these studies have evaluated the biologic properties of RMGICs, in vivo models are required to provide a more critical analysis with regard to the biocompatibility of these resin cements (Zhou et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Mesquita

Santos

Sampaio

et al. 2017

An. Acad. Bras. Ciênc.

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“…The FPR-targeting nanoprobe (cFLFLF-PEG-Oyster-800, 1.0 mg/mL) was dissolved in sterilized distilled water and the optical property of the nanoprobe was measured using a fluorescence spectrometer (RF-5301PC; Shimadzu Corporation, Tokyo, Japan), as described previously. 25 The peptide to dye conjugation ratio was determined using an ultravioletvisible spectrometer (Lambda 19 Spectrometer; PerkinElmer, Waltham, MA) according to the manufacturer's instruction and a previous publication. 26 Briefly, with a known amount of nanoprobes, the relative dye and peptide concentrations were determined using the spectrometer.…”

Section: Preparation and Characterization Of The Fpr-targeting Nanoprobementioning

confidence: 99%

“…28,29 As recently described, 100 µL of the particles or saline (as control) were injected into the subcutaneous space. 25 Limited experiments were also carried out using neutrophil-depleted mice. Neutrophil depletion was performed based on a published protocol.…”

Section: Inflammation and Infection Animal Modelmentioning

confidence: 99%

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Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Zhou

Tsai

Hong

et al. 2012

IJN

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Neutrophils play an important role in implant-mediated inflammation and infection. Unfortunately, current methods which monitor neutrophil activity, including enzyme measurements and histological evaluation, require many animals and cannot be used to accurately depict the dynamic cellular responses. To understand the neutrophil interactions around implant-mediated inflammation and infection it is critical to develop methods which can monitor in vivo cellular activity in real time. In this study, formyl peptide receptor (FPR)-targeting nearinfrared nanoprobes were fabricated. This was accomplished by conjugating near-infrared dye with specific peptides having a high affinity to the FPRs present on activated neutrophils. The ability of FPR-targeting nanoprobes to detect and quantify activated neutrophils was assessed both in vitro and in vivo. As expected, FPR-targeting nanoprobes preferentially accumulated on activated neutrophils in vitro. Following transplantation, FPR-targeting nanoprobes preferentially accumulated at the biomaterial implantation site. Equally important, a strong relationship was observed between the extent of fluorescence intensity in vivo and the number of recruited neutrophils at the implantation site. Furthermore, FPR-targeting nanoprobes may be used to detect and quantify the number of neutrophils responding to a catheter-associated infection. The results show that FPR-targeting nanoprobes may serve as a powerful tool to monitor and measure the extent of neutrophil responses to biomaterial implants in vivo.

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Real‐Time Noninvasive Monitoring of In Vivo Inflammatory Responses using a pH Ratiometric Fluorescence Imaging Probe

Tsai

Zhou

Hong

et al. 2013

Adv Healthcare Materials

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It is often difficult to continuously monitor and quantify inflammatory responses in vivo. These dynamic responses however are often accompanied by specific pH changes. A new ratiometric optical pH probe is developed by combining pH-sensitive (CypHer5E) and pH-insensitive (Oyster800) fluorescent dyes into nanoparticles for in vivo optical imaging. By taking the ratio of fluorescence intensities at different wavelengths, these nanosized sensors provide excellent measurement capabilities, and unique mapping, of the continuous in vivo pH changes for three different inflammation models. In each model a strong positive correlation is found between ratiometric pH changes and the corresponding inflammatory response measured by histological analyses. These results indicate that ratiometric imaging can provide a noninvasive, rapid, and highly sensitive optical readout for the pH-ratio changes in vivo. Furthermore this technique may be used to monitor the real-time dynamics of inflammatory processes.

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Real time monitoring of biomaterial-mediated inflammatory responses via macrophage-targeting NIR nanoprobes

Cited by 46 publications

References 64 publications

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Real‐Time Noninvasive Monitoring of In Vivo Inflammatory Responses using a pH Ratiometric Fluorescence Imaging Probe

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