Real-time monitoring of mesenchymal stem cell responses to biomaterial surfaces and to a model drug by using quartz crystal microbalance

Şeker, Şükran; Elçin, Ayşe Eser; Elçin, Yaşar Murat

doi:10.3109/21691401.2015.1089255

Cited by 10 publications

(2 citation statements)

References 45 publications

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“…The stability of AT-cut quartz under temperature change has led to a wide range of applications involving measurement of mass deposition in vacuum. TSM devices can operate in liquid and have been used for a variety of chemical or biosensor applications which include detection and analysis of proteins (serum, neurotransmitters) (Wang and Muthuswamy, 2008), antibodies as well as DNA (Ferreira et al, 2009; Li et al, 2011), self-assembled monolayer (SAMs) (Seker et al, 2016), lipids and cells (neurons, fibroblast, blood cells, neutrophils, bacteria) (Khraiche et al, 2003, 2005; Da-Silva et al, 2012; Khraiche and Muthuswamy, 2012; Zhou et al, 2012; Westas et al, 2015). The first attempts to study adhesion of cells using TSM sensors involved platelet adhesion (Matsuda, 1992).…”

Section: Introductionmentioning

confidence: 99%

Design and Development of Microscale Thickness Shear Mode (TSM) Resonators for Sensing Neuronal Adhesion

2019

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The overall goal of this study is to develop thickness shear mode (TSM) resonators for the real-time, label-free, non-destructive sensing of biological adhesion events in small populations (hundreds) of neurons, in a cell culture medium and subsequently in vivo in the future. Such measurements will enable the discovery of the role of biomechanical events in neuronal function and dysfunction. Conventional TSM resonators have been used for chemical sensing and biosensing applications in media, with hundreds of thousands of cells in culture. However, the sensitivity and spatial resolution of conventional TSM devices need to be further enhanced for sensing smaller cell populations or molecules of interest. In this report, we focus on key challenges such as eliminating inharmonics in solution and maximizing Q -factor while simultaneously miniaturizing the active sensing (electrode) area to make them suitable for small populations of cells. We used theoretical expressions for sensitivity and electrode area of TSM sensors operating in liquid. As a validation of the above design effort, we fabricated prototype TSM sensors with resonant frequencies of 42, 47, 75, and 90 MHz and characterized their performance in liquid using electrode diameters of 150, 200, 400, 800, and 1,200 μm and electrode thicknesses of 33 and 230 nm. We validated a candidate TSM resonator with the highest sensitivity and Q -factor for real-time monitoring of the adhesion of cortical neurons. We reduced the size of the sensing area to 150–400 μm for TSM devices, improving the spatial resolution by monitoring few 100–1,000s of neurons. Finally, we modified the electrode surface with single-walled carbon nanotubes (SWCNT) to further enhance adhesion and sensitivity of the TSM sensor to adhering neurons (Marx, 2003 ).

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Section: Introductionmentioning

confidence: 99%

Design and Development of Microscale Thickness Shear Mode (TSM) Resonators for Sensing Neuronal Adhesion

2019

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“…These studies have indicated that the QCM cell biosensor is suitable for evaluating cell attachment in the early stage of TE. QCM successfully detected mesenchymal stem cell (MCS) responses to biomaterial surfaces [ 157 ]. Cell adhesion depends on the surface properties of the biomaterial, such as topography, wettability, charge, and protein adlayers.…”

Section: Potential Use Of Qcm In Cartilage Tissue Engineering (Cte)mentioning

confidence: 99%

Practical Use of Quartz Crystal Microbalance Monitoring in Cartilage Tissue Engineering

Naranda

Bračič

Vogrin

et al. 2022

JFB

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Quartz crystal microbalance (QCM) is a real-time, nanogram-accurate technique for analyzing various processes on biomaterial surfaces. QCM has proven to be an excellent tool in tissue engineering as it can monitor key parameters in developing cellular scaffolds. This review focuses on the use of QCM in the tissue engineering of cartilage. It begins with a brief discussion of biomaterials and the current state of the art in scaffold development for cartilage tissue engineering, followed by a summary of the potential uses of QCM in cartilage tissue engineering. This includes monitoring interactions with extracellular matrix components, adsorption of proteins onto biomaterials, and biomaterial–cell interactions. In the last part of the review, the material selection problem in tissue engineering is highlighted, emphasizing the importance of surface nanotopography, the role of nanofilms, and utilization of QCM to as a “screening” tool to improve the material selection process. A step-by-step process for scaffold design is proposed, as well as the fabrication of thin nanofilms in a layer-by-layer manner using QCM. Finally, future trends of QCM application as a “screening” method for 3D printing of cellular scaffolds are envisioned.

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Methods for Characterization of Surface Charge and Solid–Liquid Interaction Studies of Biomaterials

Bračič

Zemljič

Plohl

et al. 2023

Functional Biomaterials

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Real-time monitoring of mesenchymal stem cell responses to biomaterial surfaces and to a model drug by using quartz crystal microbalance

Cited by 10 publications

References 45 publications

Design and Development of Microscale Thickness Shear Mode (TSM) Resonators for Sensing Neuronal Adhesion

Design and Development of Microscale Thickness Shear Mode (TSM) Resonators for Sensing Neuronal Adhesion

Practical Use of Quartz Crystal Microbalance Monitoring in Cartilage Tissue Engineering

Methods for Characterization of Surface Charge and Solid–Liquid Interaction Studies of Biomaterials

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