Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry

Líu, Hao; Zhou, Lele; Zhang, Yifan; Qian, Weiping

doi:10.1039/d2ay00811d

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…The mixture of GT with several kinds of dietary fibers was lipolyzed by lipase and monitored by the OPLI platform to determine the interactions among them. Liu et al analyzed the different hydrolysis behaviors of vegetable oils that were composed of different nutrients to provide bioavailability information for functional food design [96]. In a similar approach, the release of hydrophobic functional nutraceuticals, such as curcumin, carried by lipids can also be monitored [105], indicating the OPLI platform is capable of conducting research on controlled-release carriers.…”

Section: Lipolysismentioning

confidence: 99%

From Self-Assembly of Colloidal Crystals toward Ordered Porous Layer Interferometry

Wan

Qian

2023

Biosensors

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Interferometry-based, reflectometric, label-free biosensors have made significant progress in the analysis of molecular interactions after years of development. The design of interference substrates is a key research topic for these biosensors, and many studies have focused on porous films prepared by top-down methods such as porous silicon and anodic aluminum oxide. Lately, more research has been conducted on ordered porous layer interferometry (OPLI), which uses ordered porous colloidal crystal films as interference substrates. These films are made using self-assembly techniques, which is the bottom-up approach. They also offer several advantages for biosensing applications, such as budget cost, adjustable porosity, and high structural consistency. This review will briefly explain the fundamental components of self-assembled materials and thoroughly discuss various self-assembly techniques in depth. We will also summarize the latest studies that used the OPLI technique for label-free biosensing applications and divide them into several aspects for further discussion. Then, we will comprehensively evaluate the strengths and weaknesses of self-assembly techniques and discuss possible future research directions. Finally, we will outlook the upcoming challenges and opportunities for label-free biosensing using the OPLI technique.

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Section: Lipolysismentioning

confidence: 99%

From Self-Assembly of Colloidal Crystals toward Ordered Porous Layer Interferometry

Wan

Qian

2023

Biosensors

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“…A balance between free radical generation and antioxidant defenses is necessary for proper physiological functions. [11,12] However, when a body is subjected to potentially harmful stimuli, too many active molecules such as ROS or RNS are produced, which lead to an imbalance between the generation and scavenging of free radicals and reactive species and therefore cause oxidative damage to biomolecules. This destructive process is called oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…Research suggests that free radicals, including reactive oxygen species (ROS) and reactive nitrogen species (RNS), are involved in many physiological and pathological processes, including cell proliferation, differentiation, development, senescence, and apoptosis. A balance between free radical generation and antioxidant defenses is necessary for proper physiological functions [11,12] . However, when a body is subjected to potentially harmful stimuli, too many active molecules such as ROS or RNS are produced, which lead to an imbalance between the generation and scavenging of free radicals and reactive species and therefore cause oxidative damage to biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Characterization and Antioxidant Activity of Chrysanthemum indicum and Opisthopappus Shih during Different Growth Stages

Wei

Sun

Huang

et al. 2023

Chemistry & Biodiversity

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Recently, much attention has been devoted to natural phenolics because of their ideal structure and chemistry for free radical scavenging activities, which may play important roles in longterm health and a reduction in the risk of developing chronic degenerative diseases. Chrysanthemum indicum (C. indicum) has been widely used as a health food and as a popular herb in China for many centuries. Opisthopappus Shih (O. shih) often takes the place of its related genera, C. indicum, in functional tea or medicine prescriptions in place of origin. In this article, a comparative study on the phenolics and antioxidant activity of C. indicum and O. shih during different growth stages was investigated. The antioxidant properties of plant extracts were tested using DPPH and ABTS assays. The characterization of potential phytochemicals was carried out using Fourier transform infrared (FT-IR) spectroscopy. Total phenolics (TPC) and total flavonoid content (TFC) were measured using Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. An HPLC method was used to simultaneously quantify five phenolic compounds, including chlorogenic acid, luteolin, rutin, quercetin, and apigenin. Results indicated that the Trolox equivalent antioxidant activity (TEAC) values of C. indicum and O. shih had extremely large variations at different growth stages. The most abundant phenolics and potent antioxidant activity of two related plants appear at the early vegetative and then flowering stages. Antioxidant activities and phenolic content of O. shih were higher than those of corresponding organs of C. indicum at the same collection time. The whole plant of O. shih, especially its leaves and flowers, are good candidates for obtaining nutraceuticals and functional food ingredients.

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Real-time monitoring of papain digestion of antibodies immobilized with various strategies by optical interferometry

Wang

Zhou

et al. 2023

International Journal of Biological Macromolecules

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Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry

Abstract: The hydrolysis of four kinds of vegetable oil was monitored in real-time with ordered porous layer interferometry, which can be used to evaluate the health value of vegetable oil.

Cited by 3 publications

References 42 publications

From Self-Assembly of Colloidal Crystals toward Ordered Porous Layer Interferometry

From Self-Assembly of Colloidal Crystals toward Ordered Porous Layer Interferometry

Phenolic Characterization and Antioxidant Activity of Chrysanthemum indicum and Opisthopappus Shih during Different Growth Stages

Real-time monitoring of papain digestion of antibodies immobilized with various strategies by optical interferometry

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