Real-time multiplex analysis of four beta-thalassemia mutations employing surface plasmon resonance and biosensor technology

Feriotto, Giordana; Breveglieri, Giulia; Finotti, Alessia; Gardenghi, Sara; Gambari, Roberto

doi:10.1038/labinvest.3700106

Cited by 21 publications

(12 citation statements)

References 24 publications

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“…However, recently new approaches have been proposed. Most of them are based on surface plasmon resonance (SPR) and biosensor technologies [37]. Another quite common technique, used to investigate the reduced oxygenation capability of thalassemic RBCs, is absorption spectroscopy [38].…”

Section: Resultsmentioning

confidence: 99%

Raman Tweezers as a Diagnostic Tool of Hemoglobin-Related Blood Disorders

Rusciano

Luca

Pesce

et al. 2008

Sensors

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This review presents the development of a Raman Tweezers system for detecting hemoglobin-related blood disorders at a single cell level. The study demonstrates that the molecular fingerprint insight provided by Raman analysis holds great promise for distinguishing between healthy and diseased cells in the field of biomedicine. Herein a Raman Tweezers system has been applied to investigate the effects of thalassemia, a blood disease quite diffuse in the Mediterranean Sea region. By resonant excitation of hemoglobin Raman bands, we examined the oxygenation capability of normal, alpha- and beta-thalassemic erythrocytes. A reduction of this fundamental red blood cell function, particularly severe for beta-thalassemia, has been found. Raman spectroscopy was also used to draw hemoglobin distribution inside single erythrocytes; the results confirmed the characteristic anomaly (target shape), occurring in thalassemia and some other blood disorders. The success of resonance Raman spectroscopy for thalassemia detection reported in this review provide an interesting starting point to explore the application of a Raman Tweezers system in the analysis of several blood disorders.

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Section: Resultsmentioning

confidence: 99%

Raman Tweezers as a Diagnostic Tool of Hemoglobin-Related Blood Disorders

Rusciano

Luca

Pesce

et al. 2008

Sensors

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“…On account of their outstanding properties, PNAs have been proposed as valuable alternatives to oligonucleotide probes. [3,[22][23][24][25] Surface plasmon optical detection of PNA:DNA and PNA:RNA hybridization has already been demonstrated, [26,27] and the lack of sensitivity for unamplified DNA or RNA detection has been overcome either by coupling of spectral SPR with electrochemically based techniques (detection limit 10 pm) [28] or by use of a fluorescence-based SPR technique known as surface plasmon fluorescence spectroscopy (detection limit 200 pm). [23,25] Here we report on the possibility of using PNA probes for the ultrasensitive nanoparticle-enhanced SPRI detection of DNA sequences down to 1 fm, while maintaining a very high selectivity in the recognition of single-nucleotide mismatches, through the use of microchannel devices.…”

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confidence: 99%

Ultrasensitive Detection of DNA by PNA and Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

et al. 2008

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“…The most commonly used technique for NIPD is the qRT-PCR. Studies that based on biosensor technologies for NIPD application with cffDNA for monogenic diseases reported previously [31]. Some studies © 2019 The Author(s).…”

Section: Discussionmentioning

confidence: 99%

A New Alternative Approach for RhD Incompatibility; Determination Fetal RhD Status via Biosensor Technology

Yenilmez¹,

Kökbaş²,

Tuli³

2019

Blood Groups

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Prenatal detection of the fetal RHD status in early stage of pregnancy is observed to be useful in the management of RhD incompatibility to identify fetuses at risk of hemolytic disease. The routine use of antenatal and postnatal anti-D prophylaxis reduces the incidence of hemolytic disease of the fetus and newborn. Cell-free fetal DNA in maternal plasma is in use today for routine genotyping fetal RHD status. Fetal RhD antigens can be detected in the blood of RhDnegative pregnant women using a nanopolymer-coated biosensor and could be an alternative method for medical diagnosis. We detected RhD-positive fetal antibodies with biosensor in maternal blood of RhD-negative mothers. The electrochemical measurements were performed on a PalmSens potentiostat and corundum ceramic-based screen-printed gold electrode. The demonstrated method has a different view for the detection of fetal RhD status in early pregnancy. The biosensor technology is useful and can be carried out rapidly in clinical diagnosis. Biosensors are also reproducible methods which give results quickly compared to noninvasive fetal RHD genotyping with real-time PCR-based techniques. We suggest that this method could become an alternative part of fetal RHD genotyping from maternal plasma as a prenatal screening in the management of RhD incompatibility.

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Real-time multiplex analysis of four beta-thalassemia mutations employing surface plasmon resonance and biosensor technology

Cited by 21 publications

References 24 publications

Raman Tweezers as a Diagnostic Tool of Hemoglobin-Related Blood Disorders

Raman Tweezers as a Diagnostic Tool of Hemoglobin-Related Blood Disorders

Ultrasensitive Detection of DNA by PNA and Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

A New Alternative Approach for RhD Incompatibility; Determination Fetal RhD Status via Biosensor Technology

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