Real-time observation of glomerular hemodynamic changes in diabetic rats: Effects of insulin ARB

Li, Bing; Yao, Jian; Kawamura, Kazuko; Oyanagi-Tanaka, Yukiko; Hoshiyama, Mari; Morioka, Tetsuo; Gejyo, Fumitake; Uchiyama, Makoto; Oite, Takashi

doi:10.1111/j.1523-1755.2004.00979.x

Cited by 40 publications

(37 citation statements)

References 44 publications

(40 reference statements)

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“…Glomerular hyperfiltration, altered glomerular hemodynamics (increased ratio of afferent to efferent arteriole diameter), glomerular hypertrophy, and increased glomerular permeability to macromolecules have been confirmed (24,48). The beneficial effects of RAS blockade were also demonstrated (24,48). Other morphological and functional changes in the glomerulus (for example, in glomerulosclerosis) can be visualized using MPM (see below).…”

Section: Imaging Renal Pathologymentioning

confidence: 99%

“…Functional changes in the early phase of diabetes have been visualized in the rat kidney using MPM (48) or conventional confocal fluorescence microscopy (24). Glomerular hyperfiltration, altered glomerular hemodynamics (increased ratio of afferent to efferent arteriole diameter), glomerular hypertrophy, and increased glomerular permeability to macromolecules have been confirmed (24,48).…”

Section: Imaging Renal Pathologymentioning

confidence: 99%

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The first decade of using multiphoton microscopy for high-power kidney imaging

Peti‐Peterdi

Burford

Hackl

2012

American Journal of Physiology-Renal Physiology

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Peti-Peterdi J, Burford JL, Hackl MJ. The first decade of using multiphoton microscopy for high-power kidney imaging. Am J Physiol Renal Physiol 302: F227-F233, 2012. First published October 26, 2011 doi:10.1152/ajprenal.00561.2011In this review, we highlight the major scientific breakthroughs in kidney research achieved using multiphoton microscopy (MPM) and summarize the milestones in the technological development of kidney MPM during the past 10 years. Since more and more renal laboratories invest in MPM worldwide, we discuss future directions and provide practical, useful tips and examples for the application of this still-emerging optical sectioning technology. Advantages of using MPM in various kidney preparations that range from freshly dissected individual glomeruli or the whole kidney in vitro to MPM of the intact mouse and rat kidney in vivo are reviewed. Potential combinations of MPM with micromanipulation techniques including microperfusion and micropuncture are also included. However, we emphasize the most advanced and complex, quantitative in vivo imaging applications as the ultimate use of MPM since the true mandate of this technology is to look inside intact organs in live animals and humans. intravital imaging; juxtaglomerular apparatus; podocyte; two-photon microscopy IT HAS BEEN MORE THAN 10 YEARS since two-or three-photon excitation, collectively called multiphoton microscopy (MPM), was first applied to the study of the living kidney tissue by the Peti-Peterdi-Bell (37, 38) and Dunn-Molitoris groups (6, 42) that pioneered several applications of this powerful new imaging technology. Over the past decade, MPM has provided stunning images and real-time movies of the structure and function of the intact kidney in unparalleled spatial and temporal resolution. Because of the ability of this noninvasive imaging approach to directly visualize dynamic intrarenal processes in vivo and in near real-time without causing tissue damage, MPM has revolutionized renal physiology research and served as the perfect complement to more traditional biological and histological techniques. MPM also opened the door to studying otherwise inaccessible cell types and complex tissue structures like the glomerular podocyte (30, 39) and the juxtaglomerular apparatus (JGA) (37) in their intact environment. Applications of MPM helped to change several paradigms in renal (patho)physiology. Imaging data have been incorporated into a number of textbooks on kidney function (3,8,41), and MPM videos (30,37,39,40) have been used as visual aid and teaching material in graduate and medical student classrooms worldwide.MPM offers a state-of-the-art imaging technique superior for deep optical sectioning of living tissues. The higher resolution and minimal phototoxicity of this method permit longer time periods of continuous tissue scanning with uses in near realtime imaging of intact organs in vivo. MPM has applications far beyond the generation of superior images: dynamic intracellular processes as well as more complex physiological ...

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Section: Imaging Renal Pathologymentioning

confidence: 99%

Section: Imaging Renal Pathologymentioning

confidence: 99%

The first decade of using multiphoton microscopy for high-power kidney imaging

Peti‐Peterdi

Burford

Hackl

2012

American Journal of Physiology-Renal Physiology

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“…In rats with two-kidney, one figure-eight wrap (Grollman) hypertension, renal interstitial fluid bradykinin, NO x , and cyclic GMP were higher in the contralateral kidney than in the wrapped kidney; losartan normalized systolic blood pressure and increased renal function, interstitial fluid bradykinin, NO x , and cyclic GMP only in the contralateral kidneys, whereas PD123319 increased blood pressure and decreased these interstitial fluid substances in both kidneys, suggesting that ANG II mediates renal production of bradykinin, which in turn, releases NO and cyclic GMP via stimulation of AT 2 receptors . Li et al (2004) found that rats with diabetes mellitus exhibited glomerular hemodynamic alteration and renal hypertrophy, and treatment with either insulin or candesartan ameliorated these changes, possibly by a suppression of enhanced renal expression of eNOS in diabetic rats.…”

Section: Other Vasculaturesmentioning

confidence: 99%

Interaction of Endothelial Nitric Oxide and Angiotensin in the Circulation

2007

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“…37 Briefly, photographs of 50 glomeruli were taken using a digital camera at the time of sieving, and the surface areas were traced using a computer-assisted color image analyzer Image-Pro (version 2.0; Media Cybernetics, Silver Spring, MD). V G was calculated using the equation V G ϭ 4/3(area/) 3/2 .…”

Section: Morphometric Measurement Of Glomerular Volumementioning

confidence: 99%

Differential Expression of Nephrin According to Glomerular Size in Early Diabetic Kidney Disease

Kim

Jung

et al. 2007

Journal of the American Society of Nephrology

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Diabetic nephropathy (DN) is clinically characterized by proteinuria. Many studies tried to demonstrate a relationship between proteinuria and changes in nephrin in various forms of glomerular diseases including DN, but the results are not consistent. Glomerular hypertrophy occurs in DN, yet hypertrophy does not develop in all glomeruli concurrently. For investigation of the differences in nephrin expression according to glomerular size, glomeruli were isolated from 10 control and 10 streptozotocin-induced diabetic rats at 6 wk after the induction of diabetes by a sieving technique using sieves with pore sizes of 250, 150, 125, and 75 m. Glomeruli then were classified into large glomeruli (LG; on the 125-m sieve) and small glomeruli (SG; on the 75-m sieve) groups. Glomerular volumes were determined using an image analyzer, and mRNA and protein expression was determined by real-time PCR and Western blot, respectively. The mean volumes of diabetic LG (1.51 Ϯ 0.06 ϫ 10 6 m 3 ) and control LG (1.37 Ϯ 0.05 ϫ 10 6 m 3 ) were significantly higher than those of diabetic SG (0.94 Ϯ 0.03 ϫ 10 6 m 3 ) and control SG (0.87 Ϯ 0.03 ϫ 10 6 m 3 ; P Ͻ 0.01). Nephrin mRNA expression was significantly reduced in the diabetic LG group compared with the diabetic SG and control glomeruli groups (P Ͻ 0.05). In contrast, nephrin mRNA expression was significantly higher in the diabetic SG group compared with the diabetic LG and control glomeruli groups (P Ͻ 0.05). Even after correction for 18s rRNA and Wilms' tumor-1 mRNA expression, the differences in nephrin mRNA expression remained significant. The expression of nephrin protein showed a similar pattern to the mRNA expression. In conclusion, these data suggest that the nephrin gene is differentially expressed according to glomerular size. Furthermore, more hypertrophied glomeruli with lesser nephrin expression may be responsible for albuminuria in the early stage of DN.

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Real-time observation of glomerular hemodynamic changes in diabetic rats: Effects of insulin ARB

Cited by 40 publications

References 44 publications

The first decade of using multiphoton microscopy for high-power kidney imaging

The first decade of using multiphoton microscopy for high-power kidney imaging

Interaction of Endothelial Nitric Oxide and Angiotensin in the Circulation

Differential Expression of Nephrin According to Glomerular Size in Early Diabetic Kidney Disease

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