Real-time PCR assay for Colletotrichum acutatum sensu stricto quantification in olive fruit samples

Azevedo-Nogueira, Filipe; Gomes, Sónia; Lino, Alexandra Isabel De Amorim; Carvalho, Teresa; Martins-Lopes, Paula

doi:10.1016/j.foodchem.2020.127858

Cited by 7 publications

(1 citation statement)

References 28 publications

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“…The qRT-PCR was carried out using the primers targeting the N genes of IBV, N-F (5′-TTGAAGGTAGYGGYGTTCCTGAN-3′) and N-R (5′-CAGMAACCCACACTATACCATC-3′), which were synthesized by Bioengineering Co., Ltd., Shanghai, China. The qRT-PCR involved an initial incubation at 50 °C for 2 min followed by PCR amplification using a cycling program comprising an initial denaturation at 95 °C for 2 min and 40 amplification cycles, each consisting of denaturation at 95 °C for 15 s and annealing–extension at 60 °C for 30 s. The resulting cycle threshold (Ct) values of the reactions were determined using the Applied Biosystems Step One real-time thermocyclers (Life Technologies) [ 30 ], and the transcript levels were calculated using the 2 −ΔΔCt method [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Development and Immunoprotection of Bacterium-like Particle Vaccine against Infectious Bronchitis in Chickens

et al. 2023

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Infectious bronchitis (IB) is a major threat to the global poultry industry. Despite the availability of commercial vaccines, the IB epidemic has not been effectively controlled. The exploration of novel IBV vaccines may provide a new way to prevent and control IB. In this study, BLP-S1, a bacterium-like particle displaying the S1 subunit of infectious bronchitis virus (IBV), was constructed using the GEM-PA surface display system. The immunoprotective efficacy results showed that BLP-S1 can effectively induce specific IgG and sIgA immune responses, providing a protection rate of 90% against IBV infection in 14-day-old commercial chickens. These results suggest that BLP-S1 has potential for the development of novel vaccines with good immunogenicity and immunoprotection.

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Section: Methodsmentioning

confidence: 99%