Real-time PCR assay with an endogenous internal amplification control for detection and quantification of Anaplasma marginale in bovine blood

Kovalchuk, Svetlana N.; Babii, Anna V.

doi:10.1016/j.ttbdis.2019.101334

Cited by 4 publications

(6 citation statements)

References 25 publications

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“…The gold standard of acute anaplasmosis diagnosis involves the identification of A. marginale following the microscopic examination of blood smears [ 6 , 21 , 35 ]. Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

“…Liver pathologies are the most important in Romania cattle and have been known for many years [ 3 ], especially due to rickettsiosis and anaplasmosis transmitted by ticks [ 4 ], with significant losses in local cattle. Bovine anaplasmosis is the most widespread tick-borne disease in the world and remains a serious problem with a huge economic impact [ 5 ] because of the high morbidity and mortality in the cattle population [ 6 ]. Anaplasma marginale is one of the most common tick-borne pathogens of cattle worldwide and is more frequently detected in Europe [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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A Case Study of Photosensitivity Associated with Anaplasma spp. Infection in Cattle

Anton

Solcan

2022

Animals

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Anaplasma infection has not previously been reported as a cause of photosensitization. This case presents a five-year-old Holstein cow in her fifth month of gestation with skin injury of nonpigmented areas clearly delimited from adjacent unaffected pigmented skin. Specific lesions included alopecia, localized oedema, desquamation erythema, serous exudation, thick detached skin, fissures, crusting, and necrosis, indicating photodermatitis. Hematological abnormalities were leukocytosis with neutrophilia and normocytic hypochromic anemia. Based on a hemoparasitic examination of blood smears, the organism Anaplasma spp. was observed within the red blood cells. Biochemical analyses revealed increased serum liver enzyme activity associated with hepatocellular damage and cholestasis. This cow was RT-PCR positive for Anaplasma spp. infection. After treatment with oxytetracycline started, its condition improved within 24 h, and it recovered completely within 30 days. In this case, the underlying pathogenic mechanism of Anaplasma spp. infection facilitated the impairment of bile flow and liver function, resulting in hepatogenous photosensitization.

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“…The gold standard of acute anaplasmosis diagnosis involves the identification of A. marginale following the microscopic examination of blood smears [ 6 , 21 , 35 ]. Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Case Study of Photosensitivity Associated with Anaplasma spp. Infection in Cattle

Anton

Solcan

2022

Animals

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“…Nevertheless, for greater sensitivity and specificity in diagnosing infections of A. marginale and Babesia spp., molecular techniques are preferred, particularly for identifying persistently infected animals. Various target genes and techniques have been utilized globally over time in the quest for a gold standard test (Alvarez et al, 2019;Echaide et al, 1998;Al-Hosary, 2017;Parodi et al, 2021;Kovalchuk et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Revisão sistemática sobre diagnóstico molecular da tristeza parasitária bovina no Brasil: ocorrência e oportunidades futuras

Rahal,

Martins,

Feijó

et al. 2024

Sem. Ci. Agr.

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A bovinocultura brasileira enfrenta diversos desafios, dentre esses, as doenças transmitidas por vetores que geram prejuízos significativos pela ampla disseminação no país. As técnicas moleculares são mais sensíveis na identificação de animais persistentemente infectados. Dessa forma, o objetivo deste trabalho foi compilar dados referentes à ocorrência de Anaplasma marginale, Babesia bigemina e Babesia bovis, causadores da tristeza parasitária bovina (TPB), detectados por técnicas moleculares, identificando lacunas e oportunidades para futuras pesquisas no Brasil. Para isso, foi conduzida uma revisão sistemática, onde os principais critérios de inclusão foram: estudos realizados com amostras do Brasil; utilização de amostras de bovinos naturalmente infectados; dados exatos sobre número ou percentual de animais positivos; número de amostras por rebanho ≥ 10; e informações, pelo menos, do estado de origem das amostras. Ao todo, 38 estudos foram incluídos na revisão, utilizando-se de técnicas de PCR convencional (multiplex PCR, PCR, nPCR e snPCR) e PCR quantitativa e semiquantitativa (qPCR e HRM). As faixas de ocorrência para as espécies foram: 35 a 98,78% para A. marginale; 8,19 a 86,25% para B. bigemina; e de 7,32 a 95,47% para B. bovis. Os maiores percentuais de ocorrência de A. marginale e B. bovis foram no estado de RO, e de B. bigemina foi no estado de SP. Apesar da quantidade de dados encontrados, ainda são evidentes inúmeras oportunidades de direcionamento para futuras pesquisas, principalmente no que diz respeito ao uso de técnicas moleculares e quantificação das perdas nos rebanhos de maior ocorrência da TPB.

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“…Reports describing clinical signs due to congenital infections are scarce and show mainly anemia associated with apathy, dehydration, jaundice and respiratory problems (Girardi et al, 2012) However, such signs may contribute to the epidemiology of this disease in some areas and generate significant losses when not diagnosed in the herd, since the animals are asymptomatic and will only be identified by molecular tests, such as polymerase chain reaction (PCR) (Añez-Rojas et al, 2010;Kocan et al, 2010;Kovalchuk et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Anaplasma marginale transplacental transmission in a calf: clinical, anatomopathological, and molecular reports

Da Silva,

Bastos,

Resende Junior

et al. 2023

PRW

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Bovine anaplasmosis is caused by obligate intracellular rickettsia Anaplasma marginale transmitted by ticks Rhipicephalus microplus, hematophagous flies, and/or iatrogenically. Transplacental transmission is possible but sub-reported. In this context, the present work aimed to report a case of a calf that was born with anemia, jaundice, and neurological problems. A calf blood smear showed intraerythrocytic inclusion of A. marginale. Accordingly, the animal was treated with oxytetracycline but died three days after the start of treatment. The necropsy revealed conjunctival and subcutaneous jaundice, splenic congestion and distension, and an intensely yellow liver were noted. Histopathology showed moderate, multifocal hepatocellular degeneration, diffuse and marked cholestasis, and histiocytic necrotizing encephalitis. A. marginale infection was confirmed by PCR, and real-time PCR (qPCR). qPCR analysis showed that 3.3% of the erythrocytes in the calf's blood sample were parasitized. Thus, here we report a case of transplacental transmission A. marginale in a newborn calf.

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Real-time PCR assay with an endogenous internal amplification control for detection and quantification of Anaplasma marginale in bovine blood

Cited by 4 publications

References 25 publications

A Case Study of Photosensitivity Associated with Anaplasma spp. Infection in Cattle

A Case Study of Photosensitivity Associated with Anaplasma spp. Infection in Cattle

Revisão sistemática sobre diagnóstico molecular da tristeza parasitária bovina no Brasil: ocorrência e oportunidades futuras

Anaplasma marginale transplacental transmission in a calf: clinical, anatomopathological, and molecular reports

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