Real-time PCR assays for detection of Brucella spp. and the identification of genotype ST27 in bottlenose dolphins (Tursiops truncatus)

Interactive Analytics for Complex Cognitive Activities on Information from Annotations of Prokaryotic Genomes

“…The comments associated with eight Brucella ceti genomes (Table 1) helped us decide to further investigate the genome of Brucella ceti Cudo, a dolphin associated Brucella [32,33]. In the BioCyc pathway databases, a transcription unit is a set of one or more genes that are transcribed to produce a single messenger RNA [34].…”

Detection of Brucella spp. in bottlenose dolphins Tursiops truncatus by a real-time PCR using blowhole swabs

“…Yield and purity of DNA extracted from blowhole swab and lung samples were analyzed by Spectrophotometer ND-1000 (Thermo Fisher Scientific). Each sample was analyzed for detection of Brucella DNA using a real-time PCR assay targeting the IS711 gene as described previously (Wu et al 2014). Both blowhole swab and lung samples were extracted from the same dolphins and assays were run at the same time.…”

“…Different tissue and body fluid samples from bottlenose dolphins stranded in the coastal region of South Carolina, USA, between 2010 and 2013 were tested with the IS711 real-time PCR assay for the detection of Brucella spp. (Wu et al 2014). Brucella DNA was detected in 31% (55/178) of the clinical samples including lung, brain, brain stem, spleen, right ventricle, mesenteric and pulmonary lymph nodes, urine and amniotic fluid.…”

“…Recently, real-time PCR assays have been used for screening for Brucella spp. in cetaceans because of their high sensitivity and specificity (Sidor et al 2013, Maio et al 2014, Wu et al 2014. Different tissue and body fluid samples from bottlenose dolphins stranded in the coastal region of South Carolina, USA, between 2010 and 2013 were tested with the IS711 real-time PCR assay for the detection of Brucella spp.…”

“…Additionally, the detection of Brucella spp. was compared between real-time PCR assay and the culture method, with Brucella DNA found in 73% (27/37) of the tissue samples tested by real-time PCR, versus 24% (9/37) tested by culture, and the diagnostic sensitivity of the real-time PCR for tissue samples was 100% (Wu et al 2014). However, similar to microbiological culture, PCR assays have generally been applied mostly to dead animals because they require sampling of potentially infected internal organs (e.g.…”