Real-time PCR-based study of haemotrophic mycoplasmas in dogs from Ribeirão Preto, Brazil

Alves, TB; Faggion, SA; Santos, EV; Roberto, PG; França, SC; Fachin, AL; Marins, Mozart

doi:10.4067/s0301-732x2014000200021

Cited by 7 publications

(9 citation statements)

References 11 publications

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“…The occurrence of M. haemocanis (4.7%) found in the present study was similar to that reported in dogs sampled in an urban area (4.2%) located in the same state of Mato Grosso do Sul (Soares et al, 2016). On the other hand, the found occurrence of M. haemocanis was higher than that found in dogs sampled in the states of Pernambuco (Ramos et al, 2010), northeastern Brazil (1/205), and São Paulo (Alves et al, 2014) (3/154), but lower than that found in dogs sampled in the state of Rio Grande do Sul (17/331) (Valle et al, 2014). Only two studies reported the molecular detection of 'Candidatus M. haematoparvum' in dogs from Brazil, in the states of São Paulo (Alves et al, 2014) and Rio Grande do Sul (Valle et al, 2014).…”

Section: Discussionsupporting

confidence: 87%

“…Although hemoplasmas have been detected in domestic cats (Braga et al, 2012;Miceli et al, 2013;André et al, 2014;Santis et al, 2014) and dogs (Ramos et al, 2010;Alves et al, 2014;Valle et al, 2014;Soares et al, 2016) from several localities in Brazil, few reports have documented the occurrence of hemoplasma species in wild animals. For instance, hemoplasmas have been detected in wild carnivores maintained in captivity in zoos (Willi et al, 2007;Guimarães et al, 2007;André et al, 2011), wild rodents (Vieira et al, 2009;Conrado et al, 2015;Gonçalves et al, 2015), monkeys (Bonato et al, 2015), and deer (Grazziotin et al, 2011) in Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

et al. 2017

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Hemotropic mycoplasmas are known to cause anemia in several mammalian species. The present work aimed to investigate the occurrence of Mycoplasma spp. in wild mammals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil. Between August 2013 and March 2015, 31 Nasua nasua, 78 Cerdocyon thous, seven Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials were trapped and ectoparasites (ticks and fleas) found parasitizing the animals were collected. Mammals and ectoparasites DNA samples were submitted to conventional PCR assays for Mycoplasma spp. targeting 16S rRNA and RnaseP genes. Twenty-four N. nasua, three C. thous, two domestic dogs, one L. pardalis and one wild rodent were positive for 16S rRNA PCR protocols. Fourteen N. nasua samples were also positive in RnaseP PCR. No marsupial or arthropod showed positivity for Mycoplasma spp. The phylogenetic analyses based on 16S rRNA gene showed that all sequences obtained from dogs, two sequences obtained from C. thous and ten sequences obtained from N. nasua showed to be closely related to Mycoplasma haemocanis/Mycoplasma haemofelis species. Genotypes closely related to 'Candidatus Mycoplasma haemominutum' and Mycoplasma haemomuris were detected in the L. pardalis and in the wild rodent, respectively. Probably a novel Mycoplasma genotype, closely related to a sequence obtained from a Brazilian capybara was detected in 14 N. nasua, based on a concatenated phylogenetic analysis of 16S rRNA and RnaseP genes. The present study revealed that wild animals in southern Pantanal region, Brazil, are exposed to different species of hemoplasmas.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

et al. 2017

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“…Studies on frequency using PCR to detect M. haemocanis have been conducted in several regions of Brazil, and have presented similar results (RAMOS et al, 2010;COSTA, 2011;ALVES et al, 2014). In the present study, only samples from dogs parasitized by R. sanguineus s.l.…”

Section: Resultssupporting

confidence: 55%

“…In Brazil, canine hemotropic mycoplasmas has been reported in the northeastern region (RAMOS et al, 2010;SILVA-SANTOS et al, 2014), southeastern region (O'DWYER et al, 1997;ALVES et al, 2014), southern region (VALLE et al, 2014) and central-western regions (COSTA, 2011). In the state of Mato Grosso do Sul (MS), which is located in the central-western region of Brazil, only one survey of hemoplasmas mycoplasmas has been conducted so far, among cats (SANTIS et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil

Soares

Echeverria

Pazzuti

et al. 2016

Rev. Bras. Parasitol. Vet.

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Hemotropic mycoplasmas in dogs, such as Mycoplasma haemocanis, have been described worldwide. Recently, these pathogens have been reported to be causative agent of zoonosis. It is known that its transmission may occur through the action of blood-sucking arthropods (e.g. ticks or fleas), through blood transfusion, contaminated fomites and/or transplacentally. In Brazil, M. haemocanis is present in practically all regions and the tick Rhipicephalus sanguineus sensu lato is suspected the main vector. In the municipality of Campo Grande, state of Mato Grosso do Sul, there is little information about infection of dogs by M. haemocanis, or on the main epidemiological features associated with it. Thus, the aim of the present study was to determine the occurrence of M. haemocanis among dogs infested by ticks and to assess possible associations with some epidemiological factors. The polymerase chain reaction (PCR) and DNA sequencing were used to analyze dog blood samples (n = 94). DNA from M. haemocanis was detected in four samples. No significant associations were observed with any epidemiological parameter analyzed here. However, the results from this study confirm that this pathogen is circulating in this region and should be considered in the differential diagnosis of diseases among anemic dogs.

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“…The low prevalence of infection found is similar to that found in Campo Grande, Mato Grosso do Sul: a 4.25% infection rate among 94 dogs tested (SOARES et al, 2016). However, the infection rate in this study was higher than the observed prevalence of M. haemocanis (1.9%) and Candidatus M. haematoparvum (0.6%) in 154 dogs from Ribeirão Preto (ALVES et al, 2014). The difference between the prevalences may vary according to the population sampled, the distribution of R. sanguineus (a probable vector), or the sensitivity of the diagnostic technique employed (AQUINO et al, 2016).…”

Section: Introductionsupporting

confidence: 71%

Frequency of Mycoplasma haemocanis infection in dogs of Cuiaba, Mato Grosso, Brazil

Santos

Almeida

Cayuela

et al. 2019

SCA

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Haemotropic mycoplasmas are pleomorphic, epicellular, gram-negative bacteria, which infect red blood cells. In dogs, the acute phase of this infection may lead to haemolytic anaemia potentially precipitated by splenectomy, or by immunosuppressive drugs or diseases; however, in cases of chronic infection, the signs are inapparent. Molecular techniques and microscopic observation are the most commonly used methods to diagnose mycoplasma infection, though studies about haemotropic mycoplasma infections in dogs from Cuiabá, capital of Mato Grosso, Brazil are scarce. The objective of this study was to evaluate the frequency of mycoplasma infection, the Mycoplasma species present, and factors associated with infection in 334 dogs diagnosed using polymerase chain reaction (PCR). The frequency of infection was 5.4%, and M. haemocanis was the only species identified. No epidemiological factor investigated showed a statistically significant association with infection, including tick infestation.

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Real-time PCR-based study of haemotrophic mycoplasmas in dogs from Ribeirão Preto, Brazil

Cited by 7 publications

References 11 publications

Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

Occurrence of Mycoplasma haemocanis in dogs infested by ticks in Campo Grande, Mato Grosso do Sul, Brazil

Frequency of Mycoplasma haemocanis infection in dogs of Cuiaba, Mato Grosso, Brazil

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