Real-time PCR detection of a 16S rRNA single mutation of Helicobacter pylori isolates associated with reduced susceptibility and resistance to tetracycline in the gastroesophageal mucosa of individual hosts

Contreras, Mónica; Bénéjat, Lucie; Mujica, Heisser; Peña, J.; García-Amado, M. Alexandra; Michelangeli, Fabián A.; Lehours, Philippe

doi:10.1099/jmm.0.001051

Cited by 12 publications

(14 citation statements)

References 27 publications

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“…In our study, the main mutation was AGA 926–928 GGA (60%) which was present in North American, East Asian and Venezuelan isolates (Contreras et al, 2019; Gerrits et al, 2002; Jeng et al, 2005). The nucleotide substitutions AGA 926–928 GTA, AGA 926–928 AGC and AGA 926–928 AGT were less prevalent in our samples.…”

Section: Discussionmentioning

confidence: 48%

Mutations associated with Helicobacter pylori antimicrobial resistance in the Ecuadorian population

Zurita

Sevillano²,

Miño³

et al. 2022

Journal of Applied Microbiology

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Aims:We described the presence of Helicobacter pylori (HP) and estimated the prevalence of primary and secondary resistance using molecular detection in gastric biopsies of Ecuadorian patients.Methods and Results: 66.7% (238/357) of the patients demonstrated the presence of HP using CerTest qPCR. Of these, 69.79% (104/149) were without previous HP eradication treatment and 64.42% (134/208) with prior HP eradication treatment.The mutation-associated resistance rate for clarithromycin was 33.64% (primary resistance) and 32.82% (secondary resistance), whereas that in levofloxacin the primary and secondary resistance was 37.38% and 42%, respectively. For tetracycline and rifabutin, primary and secondary resistance was 0%. Primary and secondary resistance for metronidazole and amoxicillin could not be evaluated by genotypic methods (PCR and sequencing). Conclusions:The analysis of mutations in gyrA, 23S rRNA and 16S rRNA is useful to detect bacterial resistance as a guide for eradication therapy following failure of the first-line regimen. Significance and Impact of the Study:This study carried out in an Ecuadorian population indicates that the resistance of HP to first-line antibiotics is high, which may contribute to the high rates of treatment failure, and other treatment alternatives should be considered.

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Section: Discussionmentioning

confidence: 48%

Mutations associated with Helicobacter pylori antimicrobial resistance in the Ecuadorian population

Zurita

Sevillano²,

Miño³

et al. 2022

Journal of Applied Microbiology

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“…It can bind to the bacterial 70S rRNA and disturb the aminoacyl transfer RNA from combining with the messenger RNA and ribosome composite, resulting in the inhibition of protein synthesis. TET is an antibiotic with a low resistance rate 11,17,18 and has been used solely in the very early stage of H. pylori treatment 19 . At the very beginning of H. pylori treatment, it seemed that a single antibiotic, including TET, could work well 19 .…”

Section: Discussionmentioning

confidence: 99%

A real‐world exploratory study on the feasibility of vonoprazan and tetracycline dual therapy for the treatment of Helicobacter pylori infection in special populations with penicillin allergy or failed in previous amoxicillin‐containing therapies

et al. 2023

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Background:The treatment of Helicobacter pylori (H. pylori) infection is a challenge for those who cannot use amoxicillin. Objective:To evaluate the eradication rate and adverse effects of vonoprazan and tetracycline dual therapy as first-line and rescue treatment regimens used in special populations with penicillin allergy or failed in previous amoxicillin-containing therapies.Design: Patients enrolled were those who were H. pylori-positive with selected conditions: (1) allergic to penicillin, either naïve to treatment or had failed before; or (2) failed in previous amoxicillin-containing therapies. All enrolled patients accepted 14day vonoprazan and tetracycline dual therapy (VT dual therapy) as follows: vonoprazan (20 mg b.i.d.) and tetracycline (500 mg t.i.d. [body weight < 70 kg] or 500 mg q.i.d. [body weight ≥ 70 kg]). H. pylori status was evaluated by 13 C-urease breath test 6 weeks after treatment. All adverse effects were recorded. Some patients underwent bacterial culture and antibiotic susceptibility testing.

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“…Several commercial kits ( Cambau et al, 2009 ; Nishizawa and Suzuki, 2014 ; Redondo et al, 2018 ) are available to detect different mutations that confer resistance to clarithromycin combined or not with levofloxacin. PCR has also been applied to detect resistance to tetracycline and rifampicin by in-house protocols which are largely described in the literature ( Chisholm and Owen, 2009 ; Contreras et al, 2019 ).…”

Section: Polymerase Chain Reaction For Detection Of Resistancementioning

confidence: 99%

“…For its part, resistance to fluoroquinolones is due, in a very significant percentage, to mutations in genes gyrA and gyrB DNA gyrase, positions 86, 87, 88, and 91 ( Rimbara et al, 2012 ). Also, PCR techniques have been applied to detect tetracycline resistance ( Contreras et al, 2019 ) due to mutations in the 16S rRNA gene (positions 926–928) and rifampicin in the rpoB gene (positions 525–545 and 547) ( Chisholm and Owen, 2009 ). In the case of metronidazole, its resistance mechanisms are quite complex ( Nishizawa and Suzuki, 2014 ) and not as well defined, so for the moment, there are no commercial kits for its detection.…”

Section: Polymerase Chain Reaction For Detection Of Resistancementioning

confidence: 99%

Are molecular methods helpful for the diagnosis of Helicobacter pylori infection and for the prediction of its antimicrobial resistance?

Fernandez-Caso

Miqueleiz²,

Valdez

et al. 2022

Front. Microbiol.

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Infections produced by Helicobacter pylori (H. pylori), a spiral Gram-negative bacterium, can cause chronic gastritis, peptic ulcer, and gastric cancer. Antibiotic therapy is the most effective treatment for H. pylori infection at present. However, owing to the increasing antibiotic resistance of H. pylori strains, it has become a serious threat to human health. Therefore, the accurate diagnosis of H. pylori infections and its antibiotic resistance markers is of great significance. Conventional microbiological diagnosis of H. pylori is based on culture; however, successful isolation of H. pylori from gastric biopsy specimens is a challenging task affected by several factors and has limitations in terms of the time of response. To improve conventional methods, some molecular techniques, such as PCR, have been recently used in both invasive and non-invasive H. pylori diagnosis, enabling simultaneous detection of H. pylori and point mutations responsible for frequent antibiotic resistance. The advantages and disadvantages of molecular methods, mainly PCR, versus conventional culture for the H. pylori identification and the detection of antibiotic resistance are discussed. As expected, the combination of both diagnostic methods will lead to the most efficient identification of the H. pylori strains and the resistance patterns.

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Real-time PCR detection of a 16S rRNA single mutation of Helicobacter pylori isolates associated with reduced susceptibility and resistance to tetracycline in the gastroesophageal mucosa of individual hosts

Cited by 12 publications

References 27 publications

Mutations associated with Helicobacter pylori antimicrobial resistance in the Ecuadorian population

Mutations associated with Helicobacter pylori antimicrobial resistance in the Ecuadorian population

A real‐world exploratory study on the feasibility of vonoprazan and tetracycline dual therapy for the treatment of Helicobacter pylori infection in special populations with penicillin allergy or failed in previous amoxicillin‐containing therapies

Are molecular methods helpful for the diagnosis of Helicobacter pylori infection and for the prediction of its antimicrobial resistance?

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