Real-Time PCR Detection of Parapoxvirus DNA,

Nitsche, Andreas; Büttner, Mathias; Wilhelm, Sonja; Pauli, Georg; Meyer, Hermann

doi:10.1373/clinchem.2005.060335

Cited by 76 publications

(65 citation statements)

References 20 publications

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“…Recently, PCR targeting B2L gene has been developed for confirmatory diagnosis in sheep (Ferede, et al, 2014). A real-time PCR has also been developed based on B2Lgene to detect and differentiate from pseudocowpox virus, bovine popular stomatitis virus and seal parapox virus in clinical samples Nitsche et al, 2006). Bora et al (2012) developed a quantitative PCR assay to evaluate viable virus content in live attenuated orf vaccine.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Kumar¹,

Trivedi²,

Bhatt³

et al. 2015

Adv. Anim. Vet. Sci.

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| Contagious pustular dermatitis (CPD), also known as Orf or contagious ecthyma is an important viral disease of sheep and goats. It is mainly seen as a benign disease but malignant form has also been reported from few parts of the world. The rates of morbidity and mortality are higher, particularly in lambs and kids experiencing the disease for the first time. The causative agent of disease is Orf virus, type species of the genus Parapoxvirus belonging to family Poxviridae. The virus produces localized persistent proliferative skin lesions and affected hosts are infected repeatedly owing to its host immune evasive strategy. These viruses have been found uniformly labile to chloroform but resistant to ether and also exhibit antigenic variations among them. Clinically, the disease is enzootic and occurs in three forms viz. labial, genital / mammary and generalized forms. The incubation period in natural cases is 2-3 weeks. The disease outbreaks mostly occur between autumn and spring but its severity is more in autumn and winter than spring. The virus grows well in cell cultures of caprine, ovine and bovine origin. Confounding symptoms impose laboratory affirmation by serological assays or molecular techniques. The disease can be diagnosed by electron microscopy, serological tests and PCR/ quantitative real-time PCR. Virus specific antibody response to structural proteins of capripox and orf viruses in western-blot analysis readily differentiates these two infections. The antibody response to the 32 kDa and 26 kDa proteins of capripox viruses provides a firm basis for differentiation. Although, the role of humoral immunity is well established but probably cell mediated immunity plays a major role in recovery from natural infections. A number of inactivated and live or live modified vaccines have been tried with variable success. The duration of immunity after vaccination is controversial; outbreaks have occurred in vaccinated animals. The disease is also of public health significance as it causes infection in human beings. Keywords

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Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Kumar¹,

Trivedi²,

Bhatt³

et al. 2015

Adv. Anim. Vet. Sci.

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“…Moreover it is able to differentiate Orf virus from PCPV, BPSV and seal parapox virus (Gallina et al, 2006, Nitsche et al, 2006, Venkatesan et al, 2012. Other test based on primers and probes usage to amplify an 87 bp fragment DNA sequence of ORFV024 gene encoding an NF-κB inhibitor of Orf virus.…”

Section: Real Time Pcrmentioning

confidence: 99%

Past and Present Overview of “Orf”

Dalal¹,

Kumar²,

Chaudhary³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Detection of parapoxviruses is fundamental for the exclusion of other rash-causing illnesses, in areas both veterinary and medical (Nitsche et al, 2006).…”

Section: Figure 10: Ultrathin Section Of the Crusts Fragments Observmentioning

confidence: 99%

Detection of Parapoxvirus in goats during contagious ecthyma outbreak in Ceará State, Brazil by transmission electron microscopy techniques

Catroxo¹,

Martins²,

Souza³

2017

IJOEAR

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Abstract-Contagious ecthyma or contagious pustular dermatitis, is a viral skin disease that occurs in sheep, goats and wild ruminants and is characterized by the formation of papules, nodules or vesicles that progress into thick crusts showing two morphological forms, a mulberry (M) form with a distinctive crisscross filament pattern derived from the superimposition of upper and lower virion surfaces and a capsular (C) form caused by stain penetration and distention of the virion core, measuring 300 x 180 nm was observed. Antigen antibody reaction was increased by the colloidal gold particles. In the ultrathin sections of crusts, we verified the presence of three types of intracytoplasmic inclusion bodies, type A or Bollinger inclusion bodies, outlined by membrane, presented in it

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Real-Time PCR Detection of Parapoxvirus DNA,

Cited by 76 publications

References 20 publications

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Past and Present Overview of “Orf”

Detection of Parapoxvirus in goats during contagious ecthyma outbreak in Ceará State, Brazil by transmission electron microscopy techniques

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