Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum

Martín, María Teresa; Cobos, Rebeca; Martin, Laura E.; López-Enríquez, Lorena

doi:10.1128/aem.07360-11

Cited by 30 publications

(22 citation statements)

References 26 publications

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“…Recently, qPCR methods with great potential for use in pathogen‐free certification schemes have been set up for Phaeomoniella chlamydospora and Phaeoacremonium aleophilum, the main causal agents of Petri disease and esca in grapevine wood (Martín et al. ) and for quarantine pathogens such as Plasmopara haistedii (Ioos et al. ) and Ceratocystis platani (Pilotti et al.…”

Section: Detection Of Phytopathogenic Fungi and Oomycetes In Host Tismentioning

confidence: 99%

“…Given the high importance of an accurate detection of quarantine pathogens and the risk of false positive/negative results, a statistical procedure has been proposed to determine the cycle cut-off and the corresponding limit of detection in qPCR (Chandelier et al 2010). Recently, qPCR methods with great potential for use in pathogen-free certification schemes have been set up for Phaeomoniella chlamydospora and Phaeoacremonium aleophilum, the main causal agents of Petri disease and esca in grapevine wood (Mart ın et al 2012) and for quarantine pathogens such as Plasmopara haistedii (Ioos et al 2012) and Ceratocystis platani (Pilotti et al 2012).…”

Section: Detection Of Phytopathogenic Fungi and Oomycetes In Host Tismentioning

confidence: 99%

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Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Sanzani

Nicosia

Faedda³

et al. 2013

Journal of Phytopathology

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Quantitative polymerase chain reaction (qPCR) is a versatile technique for the accurate, sensitive, reliable and high‐throughput detection and quantification of target DNA in various environmental samples, and in recent years, it has greatly contributed to the advancement of knowledge in the plant pathology field. Indeed, this technique is ideal to evaluate inoculum threshold levels and to study the epidemiology, biology and ecology of phytopathogenic fungi and oomycetes, thus opening up new research opportunities to investigate host–pathogen interactions and to address tasks related to quarantine, eradication and biosecurity. Moreover, it can be a useful tool in breeding programs. The present review analyses the most relevant applications of qPCR for the detection and quantification of filamentous fungi and oomycetes within host tissues and in soil, air and water, along with brief paragraphs focusing on new application fields such as the detection and quantification of mycotoxigenic fungi and biocontrol agents. The high potentiality of qPCR for present and future applications is highlighted together with a critical analysis of major drawbacks that need to be corrected to definitively confirm it as a preferential routine quantitative detection method.

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Section: Detection Of Phytopathogenic Fungi and Oomycetes In Host Tismentioning

confidence: 99%

Section: Detection Of Phytopathogenic Fungi and Oomycetes In Host Tismentioning

confidence: 99%

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Sanzani

Nicosia

Faedda³

et al. 2013

Journal of Phytopathology

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“…The nested qPCR approach presented in this study has enabled the detection of up to 1 fg/μl of target DNA. Other studies have found variable limits of detection (from 1 to 250 fg) of Pch in water, wood, callusing medium and nursery soil using diverse techniques such as nested PCR, TaqMan® qPCR and multiplex Plexor™ qPCR (Martín, Cobos, Martín, & López‐Enríquez, ; Pouzoulet et al, ; Retief et al, ). In this study, the specificity of Pch quantification in vineyard soils was confirmed by way of melting curve analysis, in accordance with (Pouzoulet et al, ) who proved that this technique is essential to unequivocal detection of Pch DNA by distinguishing between specific and unspecific signals.…”

Section: Discussionmentioning

confidence: 99%

Qualitative and quantitative molecular analysis indicate the presence of Phaeomoniella chlamydospora in vineyard soils

Saccà

Manici

Caputo

et al. 2018

Journal of Phytopathology

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During the past two decades, esca disease has emerged as one of the most important diseases of grapevines. Esca is caused by complex of fungal pathogens, one of the main being Phaeomoniella chlamydospora (Pch). Since propagation material is considered one of the main infection sources of this fungus, most studies on Pch detection have focused on plant tissues and nursery soils. Conversely, due to methodological limitations encountered in retrieving this slow‐growing fungus, little is known about its true occurrence in vineyard soils. This study presents the adaptation of a molecular‐based methodology for the detection and quantification of Pch in soil samples. For this purpose, soil from the root‐explored area of esca‐foliar symptomatic and asymptomatic plants was sampled in 17 adult vineyards in two different climatic regions. Qualitative and quantitative nested PCR assays indicated the presence of Pch DNA in soil, differentiating between vineyards and regions, regardless of esca symptoms. The number of positive samples (32%) detected in the vineyards indicates that Pch can be recovered in natural soils, thus opening perspectives for the use of molecular tools in the study of the ecology and epidemiology of this fungus in soil.

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“…UniFrac compares communities by integrating the phylogenetic (P) test and the F ST test (88). The F ST test assesses genetic diversity and indicates whether less genetic diversity is present within each community when compared to both communities combined.…”

Section: Comparing Between Communitiesmentioning

confidence: 99%

Applications of Next-Generation Sequencing Technologies to the Study of the Human Microbiome

Chuang

Walsh

Yen

et al. 2014

Comprehensive Analytical Chemistry

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Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum

Cited by 30 publications

References 26 publications

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Use of QuantitativePCRDetection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples

Qualitative and quantitative molecular analysis indicate the presence of Phaeomoniella chlamydospora in vineyard soils

Applications of Next-Generation Sequencing Technologies to the Study of the Human Microbiome

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