Real-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions

Soares, Isis Capella; Pacheco, Rafael Sanches; Silva, Cleudison Gabriel Nascimento da; Santos, Rafael Salazar; Baldani, José Ivo; Urquiaga, Segundo; Vidal, Márcia Soares; Simões‐Araújo, Jean Luiz

doi:10.1007/s11104-021-05137-y

Cited by 7 publications

(2 citation statements)

References 67 publications

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“…Monitoring of bioinoculants under field conditions has increased in the last few years, revealing the dynamics of introduced microorganisms (Zhang et al 2020 , Urrea-Valencia et al 2021 ). Soares et al ( 2021 ) found differences between Brachiaria cultivars after inoculation with Azospirillum baldaniorum . In our study, the location was a determinant of H. diazotrophicus detection.…”

Section: Discussionmentioning

confidence: 99%

“…qPCR has been used to monitor the populations of various types of legume-nodulating rhizobia in the field (Maluk et al 2022 , 2023 ). Only a few long-term studies under field conditions have been performed to monitor the dynamics of PGPR in the rhizosphere soil (Zhang et al 2020 ), roots (Soares et al 2021 , Urrea-Valencia et al 2021 ), or stalks (Fernandes et al 2014 ) of different plants, including maize, Brachiaria grasses, soybean, and sugarcane.…”

Section: Introductionmentioning

confidence: 99%

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Long-term detection of Hartmannibacter diazotrophicus on winter wheat and spring barley roots under field conditions revealed positive correlations on yield parameters with the bacterium abundance

Quiroga,

Rosado-Porto,

Ratering

et al. 2024

FEMS Microbiology Ecology

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Monitoring of bioinoculants once released into the field remains largely unexplored; thus, more information is required about their survival and interactions after root colonization. Therefore, specific primers were used to perform a long-term tracking to elucidate the effect of Hartmannibacter diazotrophicus on wheat and barley production at two experimental organic agriculture field stations. Three factors were evaluated: organic fertilizer application (with and without), row spacing (15 cm and 50 cm), and bacterial inoculation (H. diazotrophicus and control without bacteria). H. diazotrophicus was detected by qPCR on the roots (up to 5 × 105 copies g−1 DW) until advanced developmental stages under field conditions during two seasons, and mostly in one farm. Correlation analysis showed a significant effect of H. diazotrophicus copy numbers on the yield parameters straw yield (increase of 453 kg ha−1 in wheat compared to the mean) and crude grain protein concentration (increase of 0.30% in wheat and 0.80% in barley compared to the mean). Our findings showed an apparently constant presence of H. diazotrophicus on both wheat and barley roots until 273 and 119 days after seeding, respectively, and its addition and concentration in the roots are associated with higher yields in one crop.

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Section: Discussionmentioning

confidence: 99%