Real-Time PCR Method Using Capturing Oligo-Immobilized PCR Tubes to Determine the Specific Gene for Soybean and Genetically Modified Soybean in Food Matrices

Harikai, Naoki; Saito, Shinroku; Abe, Miki; Kondo, Kazunari; Kitta, Kazumi; Akiyama, Hiroshi; Teshima, Reiko; Kinoshita, Kenji

doi:10.1271/bbb.80433

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…These are the cases of RT-PCR and LAMP assays. The primers are normally immobilized onto different materials depending on the technique and type of assay. , As usual in any development of a diagnostic tool, the sensing platform is first validated with standard spiked samples before experiments are done with real samples (i.e., swabs, blood, serum, and plasma samples). , The primers for RT-PCR and LAMP assays are produced by molecular biology methods. In the immunoassays, the biorecognition elements are frequently proteins (biomarkers) which will bind specifically to antibodies immobilized onto the sensing platform. ,, These devices can be applied to a large number of samples, as blood, serum, plasma, urine, and saliva.…”

Section: Sars-cov-2 Diagnosismentioning

confidence: 99%

“…The primers are normally immobilized onto different materials depending on the technique and type of assay. 51,52 As usual in any development of a diagnostic tool, the sensing platform is first validated with standard spiked samples before experiments are done with real samples (i.e., swabs, blood, serum, and plasma samples). 3,53 The primers for RT-PCR and LAMP assays are produced by molecular biology methods.…”

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confidence: 99%

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On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies

et al. 2020

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Diagnosis of COVID-19 has been challenging owing to the need for mass testing and for combining distinct types of detection to cover the different stages of the infection. In this review, we have surveyed the most used methodologies for diagnosis of COVID-19, which can be basically categorized into genetic-material detection and immunoassays. Detection of genetic material with real-time polymerase chain reaction (RT-PCR) and similar techniques has been achieved with high accuracy, but these methods are expensive and require time-consuming protocols which are not widely available, especially in less developed countries. Immunoassays for detecting a few antibodies, on the other hand, have been used for rapid, less expensive tests, but their accuracy in diagnosing infected individuals has been limited. We have therefore discussed the strengths and limitations of all of these methodologies, particularly in light of the required combination of tests owing to the long incubation periods. We identified the bottlenecks that prevented mass testing in many countries, and proposed strategies for further action, which are mostly associated with materials science and chemistry. Of special relevance are the methodologies which can be integrated into point-of-care (POC) devices and the use of artificial intelligence that do not require products from a well-developed biotech industry.

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Section: Sars-cov-2 Diagnosismentioning

confidence: 99%

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On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies

et al. 2020

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A Screening Method for the Detection of the 35S Promoter and the Nopaline Synthase Terminator in Genetically Modified Organisms in a Real-Time Multiplex Polymerase Chain Reaction Using High-Resolution Melting-Curve Analysis

Akiyama¹,

Nakamura²,

Yamada

et al. 2009

Biological & Pharmaceutical Bulletin

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To screen for unauthorized genetically modified organisms (GMO) in the various crops, we developed a multiplex real-time polymerase chain reaction high-resolution melting-curve analysis method for the simultaneous qualitative detection of 35S promoter sequence of cauliflower mosaic virus (35SP) and the nopaline synthase terminator (NOST) in several crops. We selected suitable primer sets for the simultaneous detection of 35SP and NOST and designed the primer set for the detection of spiked ColE1 plasmid to evaluate the validity of the polymerase chain reaction (PCR) analyses. In addition, we optimized the multiplex PCR conditions using the designed primer sets and EvaGreen ® as an intercalating dye. The contamination of unauthorized GMO with single copy similar to NK603 maize can be detected as low as 0.1% in a maize sample. Furthermore, we showed that the present method would be applicable in identifying GMO in various crops and foods like authorized GM soybean, authorized GM potato, the biscuit which is contaminated with GM soybeans and the rice which is contaminated with unauthorized GM rice. We consider this method to be a simple and reliable assay for screening for unauthorized GMO in crops and the processing food products.

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Pinctada fucataMantle Gene 5 (PFMG5) from Pearl Oyster Mantle Inhibits Osteoblast Differentiation

Wang

Hinshaw

Liu

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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PFMG5 (Pinctada fucata mantle gene 5) was identified from mantle tissue of the pearl oyster, Pinctada fucada (P. fucada). Here we report that PFMG5 decreased osteoblast differentiation marker alkaline phosphatase (ALP) activity and the transcript levels of osteoblast differentiation specific marker genes in MC3T3-E1 cells. PFMG5 was identified as a new molecule inhibiting osteoblast differentiation.

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Real-Time PCR Method Using Capturing Oligo-Immobilized PCR Tubes to Determine the Specific Gene for Soybean and Genetically Modified Soybean in Food Matrices

Cited by 3 publications

References 15 publications

On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies

On the Challenges for the Diagnosis of SARS-CoV-2 Based on a Review of Current Methodologies

A Screening Method for the Detection of the 35S Promoter and the Nopaline Synthase Terminator in Genetically Modified Organisms in a Real-Time Multiplex Polymerase Chain Reaction Using High-Resolution Melting-Curve Analysis

Pinctada fucataMantle Gene 5 (PFMG5) from Pearl Oyster Mantle Inhibits Osteoblast Differentiation

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