Real-time PCR quantification of Rhizoctonia solani AG-3 from soil samples

Salamone, Amy L.; Okubara, Patricia A.

doi:10.1016/j.mimet.2020.105914

Cited by 15 publications

(7 citation statements)

References 26 publications

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“…Plasmodiophora Species-specific PCR Brassica [195] Plasmodiophora PCR, SNPaSHOT Brassica [196] Powdery mildew Spectral data Cucumber [197] Powdery, down mildew Multiplex qPCR Cucumber [198] Pseudoperonospora RT PCR Cucumber [199] Rhizoctonia Multiplex RTPCR Lettuce [200] Rhizoctonia PCR, RTPCR Pulse crop [197] Rust IR spectroscopy Multiple [201] Sclerotinia Spec.specif. PCR Carrot [202] Sclerotinia RTPCR Potato [203] Sclerotinia Spec.spec. PCR Lettuce [202] Sclerotinia Spec.spec.…”

Section: Methods Species Referencementioning

confidence: 99%

Applicability of Smart Tools in Vegetable Disease Diagnostics

Ovesná

Tsiropoulos²,

Collier

et al. 2023

Agronomy

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Various diseases and pests cause serious damage to vegetable crops during the growing season and after harvesting. Growers attempt to minimize losses by protecting their crops, starting with seed and seedling treatments and followed by monitoring their stands. In many cases, synthetic pesticide treatments are applied. Integrated pest management is currently being employed to minimize the impact of pesticides upon human health and the environment. Over the last few years, “smart” approaches have been developed and adopted in practice to predict, detect, and quantify phytopathogen occurrence and contamination. Our review assesses the currently available ready-to-use tools and methodologies that operate via visual estimation, the detection of proteins and DNA/RNA sequences, and the utilization of brand-new innovative approaches, highlighting the availability of solutions that can be used by growers during the process of diagnosing pathogens.

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Section: Methods Species Referencementioning

confidence: 99%

Applicability of Smart Tools in Vegetable Disease Diagnostics

Ovesná

Tsiropoulos²,

Collier

et al. 2023

Agronomy

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“…Rhizoctonia solani Kühn (R. solani) teleomorph Thanatephorus cucumeris (Frank) Donk is a plant pathogenic fungus that causes many important economic losses on crops worldwide. 1,2 R. solani belongs to the phylum Basidiomycota, 3 and is divided into 14 anastomosis groups (AGs), namely AG-1 to AG-13 and AG-BI according to the hyphal fusion of isolates with different genotypic and phenotypic characteristics. [4][5][6] R. solani AG-3 was reported to infect crops such as soybean, 7 potato, 8,9 tobacco, 10 carrot, 11 tomato and onion.…”

Section: Introductionmentioning

confidence: 99%

“…12,13 The pathogen generally causes necrotic lesions on leaves, sheaths and stems. 14 Infection of R. solani AG-3 causes black scurf, stem and stolon canker on potatoes, 1,15 forms sclerotia on sugar beet root, 16 and stem lesions of tomato plants that are irregular in shape, water-soaked, and brown,manifesting sunken appearance. 12 Nicotiana tabacum (N.tabacum) is an annual solanaceous crop that is widely planted in China.…”

Section: Introductionmentioning

confidence: 99%

RNA interference‐based exogenous double‐stranded RNAs confer resistance to Rhizoctonia solaniAG‐3 on Nicotiana tabacum

Wang,

Guo,

Guo

et al. 2024

Pest Management Science

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BACKGROUNDRhizoctonia solani Kühn is a pathogenic fungus causing tobacco target spot disease, and leads to great losses worldwide. At present, resistant varieties and effective control strategy on tobacco target spot disease are very limited. Host‐induced gene silencing (HIGS) as well as the exogenous dsRNA can be used to suppress disease progression, and reveal the function of crucial genes involved in the growth and pathogenesis of the fungus.RESULTSThe silencing of endoPGs or RPMK1 in host plants by TRV‐based HIGS resulted in a significant reduction in disease development in Nicotiana benthamiana. In vitro analysis validated that red fluorescence signals were consistently observed in the hyphae treated with Cy3‐fluorescein‐labeled dsRNA at 12, 24, 48 and 72 h postinoculation (hpi). Additionally, application of dsRNA‐endoPGs, dsRNA‐RPMK1 and dsRNA‐PGMK (fusion of partial endoPGs and RPMK1 sequences) effectively inhibited the hyphal growth of R. solani YC‐9 in vitro and suppressed disease progression in the leaves, and quantitative real‐time PCR confirmed that the application of dsRNAs significantly reduced the expression levels of endoPGs and RPMK1.CONCLUSIONThese results provide theoretical basis and new direction for RNAi approaches on the prevention and control of disease caused by R. solani. © 2024 Society of Chemical Industry.

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“…Basidiospores of R. solani are normally present on the surface of soil, leaves, and stems and transmitted by air, causing the disease to spread to aerial parts of plants [ 4 ]. The presence of mycelia fragments and sclerotia of R. solani in the soil or on potato tubers can lead to disease susceptibility in various plant parts, causing significant damage to the plant, however, studies conducted over the years have failed to definitively establish a single inoculum source’s causal involvement in disease onset [ 11 ]. Contrary findings from various studies have shown that among the sources of inoculum, seed negatively affects plant emergence, while tuber inoculum inhibits sprout emergence and causes girdling of sprouts, leading to stem canker; soil-borne inoculum has been associated with infection of stems and stolons, as well as the development of black scurf and stem canker, underscoring the significance of both inoculum sources in disease progression [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Unveiling the Genetic Tapestry: Exploring Rhizoctonia solani AG-3 Anastomosis Groups in Potato Crops across Borders

Naqvi,

Abbas,

Farhan

et al. 2024

Plants

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The current study was carried out to screen 10 isolates (ARS-01–ARS-10) of Rhizoctonia. solani from potato tubers cv. Kuroda, which were collected from various potato fields in Multan, Pakistan. The isolates were found to be morphologically identical, as the hyphae exhibit the production of branches at right angles and acute angles often accompanied by septum near the emerging branches. Anastomosis grouping showed that these isolates belonged to AG-3. A pathogenicity test was performed against the susceptible Kuroda variety and among the isolates, ARS-05 exhibited the highest mean severity score of approximately 5.43, followed by ARS-09, which showed a mean severity score of about 3.67, indicating a moderate level of severity. On the lower end of the severity scale, isolates ARS-06 and ARS-07 displayed mean severity scores of approximately 0.53 and 0.57, respectively, suggesting minimal symptom severity. These mean severity scores offer insights into the varying degrees of symptom expression among the different isolates of R. solani under examination. PCoA indicates that the severe isolate causing black scurf on the Kuroda variety was AG-3. A comprehensive analysis of the distribution, genetic variability, and phylogenetic relationships of R. solani anastomosis groups (AGs) related to potato crops across diverse geographic regions was also performed to examine AG prevalence in various countries. AG-3 was identified as the most widespread group, prevalent in Sweden, China, and the USA. AG-5 showed prominence in Sweden and the USA, while AG-2-1 exhibited prevalence in China and Japan. The phylogenetic analysis unveiled two different clades: Clade I comprising AG-3 and Clade II encompassing AG-2, AG-4, and AG-5, further subdivided into three subclades. Although AGs clustered together regardless of origin, their genetic diversity revealed complex evolutionary patterns. The findings pave the way for region-specific disease management strategies to combat R. solani’s impact on potato crops.

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Real-time PCR quantification of Rhizoctonia solani AG-3 from soil samples

Cited by 15 publications

References 26 publications

Applicability of Smart Tools in Vegetable Disease Diagnostics

Applicability of Smart Tools in Vegetable Disease Diagnostics

RNA interference‐based exogenous double‐stranded RNAs confer resistance to Rhizoctonia solaniAG‐3 on Nicotiana tabacum

Unveiling the Genetic Tapestry: Exploring Rhizoctonia solani AG-3 Anastomosis Groups in Potato Crops across Borders

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