Real-time PCR Quantification of White Spot Syndrome Virus (WSSV) and Hepatopancreatic Parvovirus (HPV) Loads in Shrimp and Seawaters of Shrimp Ponds on the West Coast of South Korea

Jang, In‐Kwon; Gopalakannan, Ayyaru; Suriakala, Kannan; Kim, Jong-Sheek; Kim, Bong-Rae; Cho, Yeong-Rok; Meng, Xianhong; Seo, Hyeong-Chul

doi:10.5657/fas.2008.11.4.195

Cited by 7 publications

(16 citation statements)

References 16 publications

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“…The pellet was washed with 70% ethanol, and the air-dried pellet was then redissolved in 100 ll TE buffer. Three sets of HPV primers specific to genotype I [17], genotype II [18], and genotype III [7] were used to diagnose HPV infection. PCR was carried out in a 20 ll reaction mixture containing 40 ng of template, 1 9 Taq buffer, 200 lM each dNTP, 1 U ExTaq polymerase (Takara, Otsu, Japan), and 10 pmol of each primer.…”

Section: Methodsmentioning

confidence: 99%

“…After the first report in 1984 involving wild Penaeus merguiensis and Fenneropenaeus chinensis from Singapore [5], HPV was reported in 1985 from F. chinensis in Korea [6]. Since 2002, F. chinensis, the native shrimp species in Korea that is more susceptible to white spot syndrome virus (WSSV) has been largely replaced with the less susceptible Litopenaeus vannamei [7]. However, the effect of HPV infection on this new species has not yet been thoroughly investigated, although L. vannamei is also known as a host of HPV and a report on the occurrence of HPV in Korea has been published [7].…”

Section: Introductionmentioning

confidence: 99%

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Complete nucleotide sequence analysis of a Korean strain of hepatopancreatic parvovirus (HPV) from Fenneropenaeus chinensis

et al. 2011

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Hepatopancreatic parvovirus (HPV) of shrimp is distributed worldwide and the entire genome of Thailand and Indian strains (PmDNV) and one Australian strain (PmergDNV) have now been reported. The complete nucleotide sequence of a HPV strain isolated from the fleshy prawn Fenneropenaeus chinensis in Korea (FcDNV) was determined and compared to previously reported sequences. The entire genome of FcDNV contains 6,336 nucleotides, with 40% G+C content, which is the biggest of the known HPV strains. The HPV genome has three open reading frames (ORFs) with a slight overlap between the first and second ORFs. The three ORFs encode the NS2 and NS1 proteins and VP that consist of 425, 578, and 820 amino acids, respectively. Among the three proteins, the NS1 protein shows the highest sequence similarity to the NS1 protein of other known HPV strains, followed by the NS2 protein and the VP protein. Phylogenetic analyses showed that HPV can be grouped into three genotypes, as previously reported, and FcDNV can be grouped as genotype I, with HPV strains isolated in Madagascar and Tanzania. The nucleotide sequences of the noncoding regions at the 5'- and 3'-ends of the plus-strand genome showed a Y-shaped hairpin structure and simple hairpin structure, respectively.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complete nucleotide sequence analysis of a Korean strain of hepatopancreatic parvovirus (HPV) from Fenneropenaeus chinensis

et al. 2011

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“…In contrast, a recent survey of L. vannamei culture ponds showed a high incidence of another shrimp virus, hepatopancreatic parvovirus (HPV) in Korea (Jang et al . ). HPV was first reported in F. chinensis in Korea in 1985 (Lightner & Redman ), and HPV infection has been observed in both F. chinensis and L. vannamei (Jang, Suriakala, Kim, Meng & Choi ; Jang et al .…”

Section: Introductionmentioning

confidence: 97%

“…HPV was first reported in F. chinensis in Korea in 1985 (Lightner & Redman ), and HPV infection has been observed in both F. chinensis and L. vannamei (Jang, Suriakala, Kim, Meng & Choi ; Jang et al . ). HPV is a non‐enveloped icosahedral virus belonging to the Parvoviridae family in the densovirus group (Bonami, Mari, Poulos & Lightner ).…”

Section: Introductionmentioning

confidence: 97%

“…Since 2002, F. chinensis has been replaced with the Pacific white shrimp Litopenaeus vannamei , which is less susceptible to WSSV (Jang, Suriakala, Kim & Meng ), and the production of L. vannamei in 2011 was 2844 MT compared with the 18 MT of F. chinensis (http://www.fips.go.kr). Although L. vannamei is less susceptible to WSSV (Briggs, Funge‐Smith, Subasinghe & Phillips ; Rosenberry ), introduction of this new species has resulted in the occurrence of new viral diseases.…”

Section: Introductionmentioning

confidence: 99%

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Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infection

Jeeva

Kim²,

Jang

et al. 2012

Aquac Res

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A multiplex PCR kit for simultaneous detection of white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) was developed and field testing was conducted. A 604‐bp target sequence was selected from the vp28 gene of WSSV. A primer set was developed to amplify a 338‐bp DNA fragment at the junction of the NS2 and NS1 protein genes of HPV after alignment of eight sequences from different strains. Another internal positive control primer set produced a 139‐bp PCR fragment from the β‐actin gene by alignment of this gene from Litopenaeus vannamei, Fenneropenaeus chinensis and Penaeus monodon. The detection limits, tested using purified plasmids, for WSSV and HPV were 21.4 and 19.0 copies respectively. The optimum ratio for HPV, WSSV and β‐actin was 3:1:1, with an optimum annealing temperature of 57°C. Field test of the multiplex PCR with 170 L. vannamei individuals from 17 aquaculture farms showed 41.8% coinfection with WSSV and HPV, and 40.0% and 3.5% single infection with WSSV and HPV respectively. No virus‐free shrimp farm was found. Ten wild catch F. chinensis individuals showed 60% coinfection, and 40% were infected with HPV.

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Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus

Lee,

Jeon,

Kim

et al. 2023

J World Aquaculture Soc

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In this study, Pacific white shrimp (Penaeus vannamei), pond water, crab, Helice tridens, and live food samples were collected from farms in three provinces of Korea to investigate the presence of white spot syndrome virus (WSSV) and decapod hepanhamaparvovirus (DHPV). By polymerase chain reaction (PCR), DHPV was detected in all shrimp samples (83 of 83); among these, 41 samples were detected with WSSV. Amplicon sequencing and phylogenetic analyses indicated that all DHPV samples were classified as a novel genotype of DHPV. Additionally, the collected squid, polychaeta, and crab were also confirmed to be detected with novel genotypes of DHPV. By WSSV quantitative PCR, the average concentrations of viruses detected in shrimp and pond water samples were 2.58 × 107 copies/μL and 1.27 × 102 copies/μL, respectively. Simultaneous detection of WSSV and DHPV was observed in shrimp, pond water, and crab samples, and co‐infection with both viruses in shrimp could be demonstrated by histopathological analysis. Viral infection can result in significant economic losses in shrimp production. Therefore, precise monitoring and management of WSSV and DHPV prevalence in pond water, live food, and habitats are essential to preventing the spread of viral diseases in the Korean shrimp culture industry.

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Real-time PCR Quantification of White Spot Syndrome Virus (WSSV) and Hepatopancreatic Parvovirus (HPV) Loads in Shrimp and Seawaters of Shrimp Ponds on the West Coast of South Korea

Cited by 7 publications

References 16 publications

Complete nucleotide sequence analysis of a Korean strain of hepatopancreatic parvovirus (HPV) from Fenneropenaeus chinensis

Complete nucleotide sequence analysis of a Korean strain of hepatopancreatic parvovirus (HPV) from Fenneropenaeus chinensis

Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infection

Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus

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