2006
DOI: 10.1016/j.humimm.2006.02.038
|View full text |Cite
|
Sign up to set email alerts
|

Real-Time PCR Using Fluorescent Resonance Emission Transfer Probes for HLA-B Typing

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
11
0

Year Published

2007
2007
2021
2021

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 10 publications
(11 citation statements)
references
References 35 publications
0
11
0
Order By: Relevance
“…FRET probes provide information on the polymorphisms via hybridization of the sensor probe to the DNA strand. To do so, an analysis of the generation of a melting curve is required. The slow denaturalization of the probes, once they are hybridized to the amplimer, reveals which amplimers contain mismatches in relation with the sensor probe sequence (lower Tms) and which contain the full complementary sequence to the sensor probe (higher Tm) .…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…FRET probes provide information on the polymorphisms via hybridization of the sensor probe to the DNA strand. To do so, an analysis of the generation of a melting curve is required. The slow denaturalization of the probes, once they are hybridized to the amplimer, reveals which amplimers contain mismatches in relation with the sensor probe sequence (lower Tms) and which contain the full complementary sequence to the sensor probe (higher Tm) .…”
Section: Methodsmentioning
confidence: 99%
“…FRET probes provide information on the polymorphisms via hybridization of the sensor probe to the DNA strand. To do so, an analysis of the generation of a melting curve (16,18) is required. The slow denaturalization of the probes, once they are Table 1 Primers and probes used for HLA-DQ2/DQ8 genotyping and HLA-DQB1*02 homozygosity analysis HLA-DQ2/DQ8 genotyping Primers/probes…”
Section: Hla-dq2/dq8 Genotyping With Light Cycler Hybridization Fluormentioning
confidence: 99%
“…Normally high-resolution HLA genotyping requires haplotype separation for the reduction of ambiguities caused by the cis/trans difficulty and can be achieved by group-specific PCR [4] and other methods [5]. During the last decade real-time PCR had become available, and has proved to be a convenient approach for low-to medium-resolution HLA typing using the advantage of melting curve analysis based on hybridization probes [6] and SYBR Green chemistry [7].…”
Section: Introductionmentioning
confidence: 99%
“…For example, sequencebased typing (SBT) or PCR with sequence-specific oligonucleotide hybridization methods yield very precise and high resolution data, but these methods require the effort of many intensive steps and long turnaround times [5,6]. The sequence-specific primer PCR (SSP-PCR) method may offer a less intensive approach, however, it requires gel electrophoresis for the detection and nested-PCR is sometimes needed to obtain high resolution data [7]. Here, we developed a new HLA-B*3505 genotyping method by a combination of the Universal Invader assay and SSP-PCR.…”
mentioning
confidence: 99%
“…Based on this concept, real-time PCR using TaqMan or hybridization probe [7], and the loop-mediated isothermal amplification (LAMP) method [4] have been proposed for pharmacogenetic testing.…”
mentioning
confidence: 99%