Real-time polymerase chain reaction shows that density centrifugation does not always remove Chlamydia trachomatis from human semen

Al-Mously, Najwa; Cross, Neil; Eley, A.; Pacey, Allan

doi:10.1016/j.fertnstert.2008.08.128

Cited by 42 publications

(30 citation statements)

References 42 publications

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“…Possible infective organisms include chlamydia trachomatis, gardnerella vaginalis and ureaplasma urealyticum. A number of studies looked at the incidence of chlamydia positive semen of asymptomatic male partners of infertile couples (Gdoura et al, 2001(Gdoura et al, , 2007de Barbeyrac et al, 2006;Al-Mously et al, 2009). Positive results were considered to be indicative of current active infection.…”

Section: Known Medical Causes Of Male Infertilitymentioning

confidence: 99%

Medical treatment of male infertility

Rittenberg

El-Toukhy

2010

Human Fertility

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Although male factors contribute to approximately half of all cases of infertility, no specific cause can be found to explain the majority of abnormalities found in semen parameters. Specific and effective medical treatment is restricted to rare endocrine disorders. As a consequence, various empirical medical treatments are often used to treat idiopathic male infertility, with limited success. This review focuses on the rationale and current evidence on the efficacy of medical treatment of male infertility. Unless new studies provide high quality evidence in favour of medical treatment, assisted reproductive technologies will remain the mainstay of treatment of male infertility.

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Section: Known Medical Causes Of Male Infertilitymentioning

confidence: 99%

Medical treatment of male infertility

Rittenberg

El-Toukhy

2010

Human Fertility

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“…A number of studies have specifically looked at the relationship between Chlamydial infection and semen quality. While some authors have shown that C. trachomatis infection is associated with poor semen quality [7]–[8], others have claimed that it does not [9]–[10]. Some reports indicated that C. trachomatis infection is associated with a decrease in sperm concentration and motility and also with altered semen pH and reduced volume of the ejaculate [11], [12], [13]–[14].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Chlamydia trachomatis and Other Sexually Transmitted Bacteria in Semen of Male Partners of Infertile Couples in Tunisia: The Effect on Semen Parameters and Spermatozoa Apoptosis Markers

et al. 2014

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This study was undertaken to determine the prevalence of Chlamydia trachomatis, Mycoplasmas, and Ureaplasmas in semen samples of the male partners of infertile couples and to investigate whether Chlamydia trachomatis could initiate apoptosis in human spermatozoa. A total of 85 males partners of infertile couples undergoing routine semen analysis according to World Health Organization guidelines were included. Specimens were examined for the presence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvum by Real time PCR (qPCR). Semen specimens were analysed for the appearance of apoptotic markers (sperm DNA fragmentation, activated caspase 3 levels, mitochondrial membrane potential (ΔΨm)) using flow cytometry. C. trachomatis, N. gonorrhoeae, U. urealyticum, M genitalium were detected in semen samples of 13 (15.2%), 5 (5.8%), 5 (5.8%) and 3 (3.5%) male partners of infertile couples, respectively. M. hominis and U. parvum were detected in semen sample of only one patient (1.1%). The semen of infertile men positive for C. trachomatis showed lower mean of semen count and lower rapid progressive motility (category [a]) of spermatozoa compared to uninfected men with statistically significances (p = 0.02 and p = 0.04, respectively). Flow cytometry analyses demonstrated a significant increase of the mean rate of semen with low ΔΨm and caspase 3 activation of infertile men positive for C. trachomatis compared to uninfected men (p = 0.006 and p = 0.001, respectively). DNA fragmentation was also increased in sperm of infertile men positive for C. trachomatis compared to uninfected men but without statistical significances (p = 0.62). Chlamydial infection was associated to loss of ΔΨm and caspase 3activation. Thus, C. trachomatis infection could be incriminated in apoptosis induction of spermatozoa. These effects may explain the negative direct impact of C. trachomatis infection on sperm fertilizing ability.

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“…serology versus molecular methods); as well as variable and sometimes inadequate methods to assess semen quality. More recent studies (Hosseinzadeh et al, 2004;Bezold et al, 2007;Al-Mously et al, 2009), using molecular methods to detect infection, and robust methods of laboratory andrology to examine semen, have generally found that men with a current infection of C. trachomatis have poorer quality ejaculates compared than men who do not. It is unclear whether this is because of reduced levels of spermatogenesis in the presence of the bacterium, or whether infection causes an altered ejaculatory response.…”

Section: Infertilitymentioning

confidence: 99%