Real-time precise 3D measurement of micro transparent objects using All-In-Focus imaging system

Nguyen, Chanh-Nghiem; Ohara, Kenichi; Avci, Ebubekir; Takubo, Tomohito; Mae, Yasushi; Arai, Tatsuo

doi:10.1007/s12213-012-0041-5

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…Lu et al successfully performed a pick-and-place operation of biological cells in 15 seconds [5]. Nguyen et al realized the first step of the pick-and-place task (grasping) of various size microbeads (20,55, 96 µm) in 1.5 seconds [18]. Zhang et al achieved the automated pick-and-place operation of a microsphere in 6 seconds, which has been the highest speed reported so far in the literature [22].…”

Section: Introductionmentioning

confidence: 99%

High-Speed Automated Manipulation of Microobjects Using a Two-Fingered Microhand

Avci

Ohara

Nguyen

et al. 2015

IEEE Trans. Ind. Electron.

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In this paper, we present a high-speed pick-andplace method for cell-assembly applications. Besides the range of motion and accuracy, the agility of a manipulation system is an important parameter, but it has been so far underrated in the literature. To begin high-speed micromanipulation, obtaining 3D positions of both the target microobject and the end effector rapidly is necessary. Controlling the residual vibration of the end effector, which is greater at high speed, is another arduous task. Successful releasing of objects in micro-scale is also demanding. We propose a new fast detection algorithm for both the target microobject and the end effector, which will enable us to achieve high-speed control of the system. Moreover, to realize stable grasping for very fast movements, the vibration of the system is compensated, and a controllable vibration is applied to the end effectors while performing the releasing task. High-speed control of the microhand system is demonstrated with extensive experiments consisting of pick-and-place actions of 40 to 60 µm microspheres; we aimed at performing the task in 1 second.A comparison with similar studies shows the advantage of the proposed automated high-speed micromanipulation system.

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Section: Introductionmentioning

confidence: 99%

High-Speed Automated Manipulation of Microobjects Using a Two-Fingered Microhand

Avci

Ohara

Nguyen

et al. 2015

IEEE Trans. Ind. Electron.

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“…Zhe Lu proposed a micromanipulation system for the automated pick-place of single cells, which could accurately pick up a single cell, transfer the cell, and deposit it at the target location with a speed of 15-30 s per cell [40]. Based on the research by T. Arai et al [41], this paper proposes a micromanipulation method for tissue engineering, enabling rapid pick-and-place. This method combines high speed and high stability when manipulating cells, addressing a previous research gap.…”

Section: Automated Assembly Experimental Resultsmentioning

confidence: 99%

High-Speed Cell Assembly with Piezo-Driven Two-Finger Microhand

Zhao,

Deng,

Chen

et al. 2024

Applied Sciences

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In the past few decades, researchers have conducted extensive studies on cell micromanipulation methods. However, there has consistently been a lack of a micromanipulation system that excels in both precision and speed. Additionally, many of these methods rely on manual control, thus significantly reducing efficiency. In this paper, a robotized micromanipulation system employing a two-finger microhand is proposed. The microhand has a 3-DoF parallel mechanism driven by three piezoelectric actuators, enabling high-precision micromanipulation. Replacing the needle-tip end-effector with a hemispherical end-effector makes cell grasping easier and more stable. In addition, a vibration-based release method combined with gel coating is proposed to reduce the release difficulty caused by adhesion forces. Through multiple sets of experiments, we have determined the optimal grasping and releasing conditions while balancing precision, stability, and damage degree to cells. An automated cell assembly strategy based on microscopic visual feedback and pick-and-place path planning is proposed to achieve the robotized high-speed cell array. Hela cells were chosen as the operation objects, achieving a 95% success rate in grasping and a 97% success rate in releasing. A “T” letter array formed by cells was successfully assembled with an average grasp and release time of less than 0.8 s and an assembly accuracy of 4.5 μm for a single cell. This study holds significant implications for the fields of biology and medicine, presenting potential applications in tissue engineering.

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“…It was obvious that the obtained CNC had a higher whiteness index of 80%, which was remarkably high for CNC that was obtained from waste newspaper compared with those obtained from natural sources of material with the highest degree of whiteness up to 85% [20,21]. For a preliminary analysis of the morphology of the fibers, their best-focused microscopic image was obtained by using an integrated autofocusing module that was developed on the principle of All-In-Focus algorithm [22]. It was obvious that the diameter of the fibers were reduced after two-step pretreatment (figures 3(a)-(c)).…”

Section: Transmittance Determinationmentioning

confidence: 99%

Extraction of thermally stable cellulose nanocrystals in short processing time from waste newspaper by conventional acid hydrolysis

Van‐Pham

Pham

Tran

et al. 2020

Mater. Res. Express

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Motivated by many exclusive and useful features of cellulose nanocrystals (CNC) and the underutilized resource of waste newspaper, this study aimed to extract CNC from waste newspapers by means of alkali and bleaching treatments followed by acid hydrolysis. The morphological structure of the obtained CNC was analysed by optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) methods. The remarkable removal of surface contamination and the reduction in fiber diameter during alkali and bleaching treatment were observed and the nano-dimension of the extracted CNC was revealed with the average diameter of 12.3±2.8 nm. Characterization of the extracted CNC showed a high whiteness index of 80%, and high transparency of about 80% of the light at 600 nm calculated for a 0.02 mm thick nanocellulose film. Fourier transform infrared spectroscopy (FTIR) indicated that lignin, hemicellulose and other coloring agents were successfully removed. A comparably high crystallinity index of 80.15% was calculated from x-ray diffraction data. Thermogravimetric analysis showed that the product had a typical maximum thermal degradation at 300°C. The analysis results indicated the successful extraction of good CNC from waste newspaper with the shortest processing time ever reported for acid hydrolysis with conventional alkali and bleaching pretreatment. The findings also strongly support for further research of nanocomposites reinforced by CNC produced from waste newspaper.

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Real-time precise 3D measurement of micro transparent objects using All-In-Focus imaging system

Cited by 6 publications

References 21 publications

High-Speed Automated Manipulation of Microobjects Using a Two-Fingered Microhand

High-Speed Automated Manipulation of Microobjects Using a Two-Fingered Microhand

High-Speed Cell Assembly with Piezo-Driven Two-Finger Microhand

Extraction of thermally stable cellulose nanocrystals in short processing time from waste newspaper by conventional acid hydrolysis

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