The vibration signal of a diesel engine contains a large amount of fault information, and an accurate collection of vibration signals will provide reliable data support for diesel engine condition monitoring. This paper designs a diesel engine vibration signal acquisition system based on the powerful Zynq chip. IEPE piezoelectric acceleration sensors are selected to collect diesel engine vibration signals, and a signal conditioning circuit is designed to filter out interference during signal transmission using the characteristics of differential signals and to adjust the voltage amplitude of the signal to ensure compliance with the input voltage level requirements of the AD conversion module. Considering the excellent performance of the AD7771, it is selected as the AD conversion chip, and the peripheral circuit design is completed. With the help of Vivado software, the software and hardware design based on the Zynq chip is completed, controlling the AD7771 to sample 8 channels of vibration signals. Finally, a signal conditioning circuit simulation model is established in Multisim 14.1 software to verify its good performance, and upper computer software is developed to monitor the fault information of the diesel engine.