Real-Time QCM-D Monitoring of the Adsorption–Desorption of Expansin on Lignin

Cui, Mei; Duan, Yuhao; Ma, Yuanyuan; Al-Shwafy, Khaled W.A.; Liu, Yudong; Zhao, Xudong; Huang, Renliang; Qi, Wei; He, Zhimin; Su, Rongxin

doi:10.1021/acs.langmuir.0c00104

Cited by 12 publications

(6 citation statements)

References 43 publications

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“…Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to investigate cellulase–lignin affinity, but the results obtained were not quantitative [ 18 ]. In recent years, the quartz crystal microbalance with dissipation monitoring (QCM-D) technique has been relied upon to investigate kinetic adsorption behavior for different polymers in real time [ 19 , 20 ]. However, the spectra obtained from QCM-D for kinetic adsorption between lignin and cellulase are hard to repeat, due to the high sensitivity of QCM-D, which is greatly affected by lignin film preparation.…”

Section: Introductionmentioning

confidence: 99%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

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Section: Introductionmentioning

confidence: 99%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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“…62 Herein we employ QCM to quantify the adsorption of PGMA 51 −PBzMA y nanoparticles (y = 200, 400, or 800) onto PAGEO5MA brushes by determining the adsorbed mass per unit area (Γ, mg m −2 ). 84,85 With QCM, we first demonstrate that the aldehyde groups within the hydrophilic brush chains are essential for strong nanoparticle adsorption. PGEO5MA and PAGEO5MA brushes were grown from separate QCM sensors before being exposed in turn to the same 1.0% w/w dispersion of PGMA 51 −PBzMA 800 nanoparticles at pH 4.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…QCM is widely used for the construction of various types of biosensors − and can also be used to characterize the viscoelastic properties of thin polymer films such as polymer brushes. − We have recently employed QCM to quantify the extent of adsorption of either diblock copolymer nanoparticles or nanosized oil droplets onto model soft substrates ,, and for the conjugation of a globular protein to a PAGEO5MA brush via Schiff base chemistry . Herein we employ QCM to quantify the adsorption of PGMA 51 –PBzMA y nanoparticles ( y = 200, 400, or 800) onto PAGEO5MA brushes by determining the adsorbed mass per unit area (Γ, mg m –2 ). , …”

Section: Resultsmentioning

confidence: 99%

Controlling Adsorption of Diblock Copolymer Nanoparticles onto an Aldehyde-Functionalized Hydrophilic Polymer Brush via pH Modulation

Astier,

Johnson,

Norvilaite

et al. 2024

Langmuir

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Sterically stabilized diblock copolymer nanoparticles with a well-defined spherical morphology and tunable diameter were prepared by RAFT aqueous emulsion polymerization of benzyl methacrylate at 70 °C. The steric stabilizer precursor used for these syntheses contained pendent cis-diol groups, which means that such nanoparticles can react with a suitable aldehyde-functional surface via acetal bond formation. This principle is examined herein by growing an aldehydefunctionalized polymer brush from a planar silicon wafer and studying the extent of nanoparticle adsorption onto this model substrate from aqueous solution at 25 °C using a quartz crystal microbalance (QCM). The adsorbed amount, Γ, depends on both the nanoparticle diameter and the solution pH, with minimal adsorption observed at pH 7 or 10 and substantial adsorption achieved at pH 4. Variable-temperature QCM studies provide strong evidence for chemical adsorption, while scanning electron microscopy images recorded for the nanoparticle-coated brush surface after drying indicate mean surface coverages of up to 62%. This fundamental study extends our understanding of the chemical adsorption of nanoparticles on soft substrates.

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“…In addition, the ease of QCM-D sensor surface modification permits versatility and the investigation of different chemical and physical interactions. Hence, QCM-D has been widely utilized to investigate the real-time chemical and physical adsorption and desorption of various compounds from the QCM-D sensor surfaces. , With QCM-D versatility, understanding API interaction and removal from common pharmaceutical manufacturing plant surface materials can be accomplished by studying the frequency and dissipation change of the crystal sensor.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Adsorption and Desorption of Sitagliptin Phosphate Monohydrate on SS2343, Glass, and PTFE Using QCM-D and Raman Spectroscopy

et al. 2022

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Cross-contamination during pharmaceutical drug manufacturing can result in expensive recalls. To counter that, companies spend significant time and resources to ensure equipment cleanliness, often relying on the compound solubility data in various solvents as the main indicator of cleaning success. The aim of this work is to provide an alternative way to analyze the fouling and cleaning of surfaces in pharmaceutical manufacturing processes by using the quartz crystal microbalance with dissipation (QCM-D) and Raman spectroscopy. In this study, we chose an active pharmaceutical ingredient (API), sitagliptin phosphate monohydrate (SIT), as the model drug compound and observed its adsorption and desorption on stainless steel (SS2343), borosilicate glass (glass), and polytetrafluoroethylene (PTFE) surfaces. SIT was selected as the model API since it is a product manufactured on a large scale and is part of the widely used dipeptidyl peptidase-IV inhibitor class of oral hypoglycemics used to treat type 2 diabetes mellitus, while the chosen surfaces mimic the wall materials of manufacturing equipment and components such as reactors, transfer lines, and valves. Both the QCM-D and Raman spectroscopy results show the highest physisorption on PTFE, followed by SS2343 and glass. Additionally, QCM-D revealed a harder removal of SIT from SS2343 compared to glass and PTFE. Raman analysis of the chemical interactions disclosed C−F and C�O bond interactions between SIT and the surfaces, and the lack of a peak shift suggested dipole−dipole interactions. Furthermore, contact angle measurements indicate that hydrophobic attraction contributed to SIT adhesion to the PTFE surface. Subsequently, SIT coverage upon deposition on a PTFE surface has a significantly smaller surface area than on SS2343 and glass due to surface hydrophobicity, hence resulting in a longer removal time. These results provide a practical use of QCM-D and Raman spectroscopy to enhance the understanding of fouling and improve the cleaning of complex small molecules on relevant surfaces during the pharmaceutical manufacturing process.

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Real-Time QCM-D Monitoring of the Adsorption–Desorption of Expansin on Lignin

Cited by 12 publications

References 43 publications

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Controlling Adsorption of Diblock Copolymer Nanoparticles onto an Aldehyde-Functionalized Hydrophilic Polymer Brush via pH Modulation

Understanding the Adsorption and Desorption of Sitagliptin Phosphate Monohydrate on SS2343, Glass, and PTFE Using QCM-D and Raman Spectroscopy

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