“…FIG1 displays the largest fold induction upon pheromone stimulation (Roberts et al , ). In this assay, the FIG1 promoter drives the expression of a small peptide, which interacts with a fluorescent protein and promotes its recruitment in the nucleus (Fig A, Appendix Fig S2B, Aymoz et al , ). Upon stimulation, the cells activate the mating MAPKs a few minutes after stimulation, as previously described (Yu et al , ; Nagiec & Dohlman, ; Durandau et al , ).…”