Real-time reverse transcription polymerase chain reaction method for detection of <i>Canine distemper virus</i> modified live vaccine shedding for differentiation from infection with wild-type strains

Wilkes, Rebecca P.; Sánchez, Elena González; Riley, Matthew C.; Kennedy, Melissa

doi:10.1177/1040638713517232

Cited by 34 publications

(46 citation statements)

References 22 publications

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“…Real‐time reverse transcriptase PCR has been used to amplify canine distemper virus RNA in blood, urine, and conjunctival swabs after administration of SQ MLVx . In this study, the PCR panel did not amplify distemper virus RNA from nasal or pharyngeal swabs.…”

Section: Distribution Of Positive Results For Nucleic Acids Of Adenovmentioning

confidence: 80%

Adenovirus 2,Bordetella bronchiseptica, and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines

Ruch-Gallie

Moroff²,

Lappin

2015

Veterinary Internal Medicne

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BackgroundCanine adenovirus 2, parainfluenza, and Bordetella bronchiseptica cause respiratory disease in dogs, and each has a modified live intranasal vaccine available. Molecular diagnostic assays to amplify specific nucleic acids are available for each of these agents. If positive molecular diagnostic assay results are common after vaccination, the positive predictive value of the diagnostic assays for disease would be decreased.ObjectiveTo determine the impact of administration of commercially available modified live topical adenovirus 2, B. bronchiseptica, and parainfluenza vaccine has on the results of a commercially available PCR panel.AnimalsEight puppies from a research breeding facility negative for these pathogens.MethodsBlinded prospective pilot study. Puppies were vaccinated with a single dose of modified live topical adenovirus 2, B. bronchiseptica, and parainfluenza and parenteral dose of adenovirus 2, canine distemper virus, and parvovirus. Nasal and pharyngeal swabs were collected on multiple days and submitted for PCR assay.ResultsNucleic acids of all 3 organisms contained in the topical vaccine were detected from both samples multiple times through 28 days after vaccination with higher numbers of positive samples detected between days 3 and 10 after vaccination.Conclusions and Clinical ImportanceVaccine status should be considered when interpreting respiratory agent PCR results if modified live vaccines have been used. Development of quantitative PCR and wild‐type sequencing are necessary to improve positive predictive value of these assays by distinguishing vaccinate from natural infection.

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Section: Distribution Of Positive Results For Nucleic Acids Of Adenovmentioning

confidence: 80%

Adenovirus 2,Bordetella bronchiseptica, and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines

Ruch-Gallie

Moroff²,

Lappin

2015

Veterinary Internal Medicne

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“…For this purpose, 110 clinical samples submitted to the University of Tennessee, College of Veterinary Medicine (UTCVM), Clinical Virology Lab from 2010 to 2014 were tested by RT-iiPCR and real-time RT-PCR. These samples included strains from 4 different clades of the phylogenetic trees derived from sequences of the H gene and M-F intergenic region [48]. 100% agreement was found between the two reactions (56 positive and 54 negative) ( Table 3).…”

Section: Validation Of CDV Rt-iipcr Using Clinical Samplesmentioning

confidence: 98%

Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

et al. 2014

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Background: Canine distemper virus (CDV) has been associated with outbreaks of canine infectious respiratory disease in shelters and boarding kennel environments. POCKIT TM Nucleic Acid Analyzer is a field-deployable device capable of generating automatically interpreted insulated isothermal polymerase chain reaction (iiPCR) results from extracted nucleic acid within one hour. In this study, reverse transcription iiPCR (RT-iiPCR) was developed to facilitate point-of-need diagnosis of CDV infection. Results: Analytical sensitivity (limit of detection 95%) of the established CDV RT-iiPCR was about 11 copies of in vitro transcribed RNA per reaction. CDV RT-iiPCR generated positive signals from CDV, but not Bordetella bronchiseptica, canine parvovirus, canine herpesvirus, canine adenovirus 2, canine influenza virus (subtype H3N8), canine parainfluenza virus, and canine respiratory coronavirus. To evaluate accuracy of the established reaction in canine distemper clinical diagnosis, 110 specimens from dogs, raccoons, and foxes suspected with CDV infection were tested simultaneously by CDV RT-iiPCR and real-time RT-PCR. CDV RT-iiPCR demonstrated excellent sensitivity (100%) and specificity (100%), compared to real-time RT-PCR.

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“…A more rapid diagnostic technique for the detection of CDV is real-time RT-PCR [107][108][109]. Real-time RT-PCR is used for both diagnostics and research and is especially useful for pathogen detection.…”

Section: Molecular Assaysmentioning

confidence: 99%

Advances in canine distemper virus pathogenesis research: a wildlife perspective

Loots

Mitchell

Dalton

et al. 2017

Journal of General Virology

105

115

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Canine distemper virus (CDV) has emerged as a significant disease of wildlife, which is highly contagious and readily transmitted between susceptible hosts. Initially described as an infectious disease of domestic dogs, it is now recognized as a global multi-host pathogen, infecting and causing mass mortalities in a wide range of carnivore species. The last decade has seen the effect of numerous CDV outbreaks in various wildlife populations. Prevention of CDV requires a clear understanding of the potential hosts in danger of infection as well as the dynamic pathways CDV uses to gain entry to its host cells and its ability to initiate viral shedding and disease transmission. We review recent research conducted on CDV infections in wildlife, including the latest findings on the causes of host specificity and cellular receptors involved in distemper pathogenesis.

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Real-time reverse transcription polymerase chain reaction method for detection of Canine distemper virus modified live vaccine shedding for differentiation from infection with wild-type strains

Cited by 34 publications

References 22 publications

Adenovirus 2,Bordetella bronchiseptica, and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines

Adenovirus 2,Bordetella bronchiseptica, and Parainfluenza Molecular Diagnostic Assay Results in Puppies After vaccination with Modified Live Vaccines

Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

Advances in canine distemper virus pathogenesis research: a wildlife perspective

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