Presently, the quantification of water content in ultra high temperature (UHT) processed milk predominantly relies on destructive methods such as pH and conductivity measurements. These methodologies, while effective, are time-consuming and necessitate specific experiential knowledge. This study delineates a non-destructive alternative employing ultrasound technology for monitoring water content in UHT milk. The interplay between electrical conductivity and water content in UHT milk was scrutinized and juxtaposed with ultrasound measurements. Ultrasound parameters, specifically pulse velocity and attenuation coefficient, were scrutinized as functions of water content at varying test temperatures. This approach elucidated the evolution of the physicochemical attributes of UHT milk with unprecedented clarity. The findings underscore the feasibility of determining water content via temperature-dependent ultrasound readings of velocity and attenuation. Importantly, this study substantiates the potential of ultrasound technology as a practical, non-destructive replacement for conventional methodologies in the dairy industry.