Realization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis

Abstract

A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.

Mass Spectrometry Imaging of Biomolecular Information

“…Alternative enzymes were also used, such as elastase, pepsin, PGNase F and chymotrypsin 150 . Immobilization of trypsin on graphene nanosurfaces has also been described recently 151 .…”

Recent advances in applications of nanomaterials for sample preparation

“…Ju et al [28] modified CNTs with aminophenylboronic acid (APBA) for glycopeptide enrichment via the reversible covalent bonding between glycans and boronic acids. In addition to specific enrichment, immobilization of protease on CNPs has also been developed for fast protein digestion in proteome analysis [29]. Direct use of pristine or chemically modified CNPs, however, often becomes troublesome because of some intrinsic properties of CNPs.…”

Recent progress in application of carbon nanomaterials in laser desorption/ionization mass spectrometry

“…To improve the selectivity of the SELDI probe [89][90][91] 3. To endow graphene with multiple functions (e.g., enzyme [92][93][94][95])…”