Reaming in treatment of non-unions in long bones: cytokine expression course as a tool for evaluation of non-union therapy

Westhauser, Fabian; Zimmermann, G.; Moghaddam, Sanaz; Bruckner, Tom; Schmidmaier, Gerhard; Biglari, Bahram; Moghaddam, Arash

doi:10.1007/s00402-015-2253-3

Cited by 26 publications

(35 citation statements)

References 40 publications

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“…A decrease in the porous surface is induced by embedding structure within the cavities-bone formation in pores reduces SS. 2 TV, SV, and P decreased more in BM0. TV outlines the whole implant, including soft tissue; SV represents only the scaffold itself.…”

Section: Discussionmentioning

confidence: 84%

“…2,4 It has been shown in previous studies that ectopically implanted constructs only provide bone formation and do not induce cartilage tissue growth; bone formation within the implants is induced by the implanted human MSC and does not come from the host itself. 15 By comparing RIA0 to BM0, we could detect a significant decrease of rPN in RIA0, but not in BM0.…”

Section: Discussionmentioning

confidence: 99%

“…Insulin-like growth factor (IGF)-1 showed promising results in clinical studies and could also be evaluated with our model. 2,17 Furthermore, b-TCP-granules should be replaced by either solid b-TCP-scaffolds to provide a more standardized model or alternative bone substitutes like bioactive glasses, which can be tested within the experimental model described. 7,18 Another promising way to stimulate bone formation clinically could be the use of the patient's own plateletrich plasma (PRP), containing a physiological mix of a lot of growth and differentiation factors.…”

Section: Discussionmentioning

confidence: 99%

“…2 We decided to use an appropriate subset of parameters as described above (Table 1). TV showed significant declines in all groups.…”

Section: Quantitative Mct-analysismentioning

confidence: 99%

“…1,2 Tissue engineering protocols for the treatment of bone defects include the use of autologous cell populations in combination with differentiation factors such as bone-morphogenetic-protein 7 (BMP-7) to promote local bone healing processes. 3 The gold standard for harvesting mesenchymal stem cells (MSC) is iliac crest bone grafting.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Westhauser

Höllig

Reible

et al. 2016

Journal of Orthopaedics

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…2 We decided to use an appropriate subset of parameters as described above (Table 1). TV showed significant declines in all groups.…”

Section: Quantitative Mct-analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Westhauser

Höllig

Reible

et al. 2016

Journal of Orthopaedics

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High‐mobility group box‐B1 (HMGB1) mediates the hypoxia‐induced mesenchymal transition of osteoblast cells via activating ERK/JNK signaling

Qiu¹,

Chen²,

Zeng³

et al. 2016

Cell Biology International

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High-mobility group box 1 (HMGB1) is a nuclear protein that involves the binding with DNA and influences chromatin regulation and transcription. HMGB1 activates monocytes and neutrophils, which are involved in inflammation during wounding. In this study, we investigated the promotion of HMGB1 under hypoxia and determined the regulatory role of HMGB1 on the fibrosis of mouse osteoblast-like MC3T3-E1 cells or of human osteoblast MG-63 cells. Results demonstrated that HMGB1 expression was significantly upregulated in MC3T3-E1 or MG-63 cells under hypoxia. We also found that treatment with 10 and 100 ng/mL of HMGB1 significantly promoted the fibrosis-associated markers such as Collagen I, α-SMA, whereas downregulated the E-cadherin, indicating the differentiation of MC3T3-E1 or MG-63 cells into fibroblast cells. Further investigation indicated that the HMGB1 treatment markedly activated the mitogen-activated protein kinases (MAPKs), including extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (p38) phosphorylation, as well as nuclear factor (NF)-κB nuclear translocation. On the other side, using specific inhibitors and shRNAs of protein kinases, we observed that repression of ERK, JNK, p38, and NF-κB all inhibited HMGB1-induced cellular differentiation and migration of MC3T3-E1 cells. In addition, knocking down of advanced glycation end products (RAGE) but not Toll-like receptor (TLR)2 and TLR4 by shRNAs attenuated HMGB1-induced myofibroblast differentiation and migration. In conclusion, our study demonstrated that HMGB1 induced the fibrosis of osteoblasts in vitro via activating the RAGE-MAPK and NF-κB interaction signaling pathways.

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Gelatin‐coating increases in‐vivo bone formation capacity of three‐dimensional 45S5‐bioactive glass‐based crystalline scaffolds

Westhauser

Senger

Obert

et al. 2018

J Tissue Eng Regen Med

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Recent studies have demonstrated that surface characteristics, porosity, and mechanical strength of three‐dimensional 45S5‐type bioactive glass (BG)‐based scaffolds are directly correlated with osteogenic properties. Three‐dimensional BG‐based scaffolds obtained from maritime natural sponges (MNSs) as sacrificial templates exhibit the required morphological properties; however, no in vivo data about the osteogenic features are available. In this study, uncoated (Group A) and gelatin‐coated (Group B) crystalline MNS‐obtained BG‐based scaffolds were evaluated mechanically and seeded with human mesenchymal stem cells prior to subcutaneous implantation in immunodeficient mice. Before implantation and after explantation, micro‐computed tomography scans were conducted, and scaffolds were finally subjected to histomorphometry. Scaffolds of both groups showed bone formation. However, Group B scaffolds performed distinctly better as indicated by a significant increase in scaffold volume (8.95%, p = 0.039) over the implantation period compared with a nonsignificant increase of 5.26% in Group A scaffolds in micro‐computed tomography analysis. Furthermore, percentage bone area was 10.33% (±1.18%) in the Group B scaffolds, which was significantly (p = 0.007) higher compared with the 8.53% (±0.77%) in the Group A scaffolds in histomorphometry. Compressive strength was enhanced significantly by gelatin coating (9 ± 2 vs. 4 ± 1 MPa; p = 0.029). The presence of gelatin on the remnant parts was verified by scanning electron microscopy and X‐ray spectroscopy, demonstrating the coatings' resilience. MNS‐obtained BG‐based scaffolds were thus confirmed to exhibit osteogenic properties in vivo that can significantly be enhanced by gelatin coating.

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Reaming in treatment of non-unions in long bones: cytokine expression course as a tool for evaluation of non-union therapy

Cited by 26 publications

References 40 publications

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

High‐mobility group box‐B1 (HMGB1) mediates the hypoxia‐induced mesenchymal transition of osteoblast cells via activating ERK/JNK signaling

Gelatin‐coating increases in‐vivo bone formation capacity of three‐dimensional 45S5‐bioactive glass‐based crystalline scaffolds

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