Laparoscopic appendectomy also known as minimally invasive surgery has revolutionized the field of surgery by offering patients less invasive alternatives to traditional open procedure. However, anatomical variations in the location of appendix can prove challenging for laparoscopic surgeons. Objective: To evaluate the effectiveness and challenges of laparoscopic appendectomy in patients with anatomical variations in the location of the appendix. Methods: It was a cross-sectional study conducted at Jinnah international hospital Abbottabad KPK Pakistan from January 2023 to December 2023. After screening through the selected criteria 91 patients were included in this study. Data were entered and analyzed using SPSS version 24.0. P-values of ≤0.05 will be considered statistically significant. Results: The mean age of the study population was 49.2 ± 5.4years. Among the 91 patients analyzed, the anterior position was observed in 54 individuals, pelvic position observed in 10 patients, the retrocecal position observed in 19 patients, and the subhepatic position observed in 8 patients. Among patients with a retrocecal appendiceal position 19 (13.6%) experienced appendiceal rupture, while 2.9% had appendiceal bleeding. A notable proportion (11.11%) required conversion to open surgery. Among patients with a subhepatic appendiceal position 8 (17.1%) experienced appendiceal rupture, while 1.9% had appendiceal bleeding. A considerable proportion (12.5%) required conversion to open surgery. Conclusions: In conclusion, laparoscopic appendectomy, while advantageous over open surgery in many aspects, presents specific challenges when dealing with anatomical variations of the appendix, particularly in the retrocecal and subhepatic positions. The study found that these variations are associated with higher rates of intraoperative complications and conversion to open surgery.