Rearing of the Adult Echinococcus Multilocularis Leuckart, 1863, From Sterile Larvae From Man

Rl, Rausch; Jf, Wilson

doi:10.4269/ajtmh.1973.22.357

Cited by 18 publications

(2 citation statements)

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“…In humans, however, sterile vesicles (lacking protoscolices) that are usually of small size are dispersed in a dense matrix of collagen that has replaced hepatic tissue. In collecting material for inoculation, slices of the lesion are examined for cavities containing membranes (figure 1), which are translucent and often folded [28]. The membranes are removed by means of fine-pointed forceps and placed in saline, after which aliquots are prepared for inoculation into the rodents (three rodents were routinely inoculated, unless the quantity of membranes was insufficient).…”

Section: Methodsmentioning

confidence: 99%

Parasiticidal Effect of Chemotherapy in Alveolar Hydatid Disease: Review of Experience with Mebendazole and Albendazole in Alaskan Eskimos

Wilson

Rausch

McMahon

et al. 1992

Clinical Infectious Diseases

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114

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Evidence that the larval stage of Echinococcus multilocularis in humans is killed by chemotherapy is presented in a review of our 17-year experience with treatment of alveolar hydatid disease in Alaska. The efficacy of chemotherapy was assessed with use of an in vivo assay of parasite viability by means of inoculation of voles, immunohistochemical tests, and histopathologic findings. Of 14 tests performed for nine patients, 12 in vivo assays (86%) were negative after chemotherapy, while only two (17%) of 12 vole tests for seven untreated patients were negative. Regression of arrest of growth of metastatic and primary hepatic lesions, together with their partial-to-complete calcification and prolonged survival times has been observed among patients treated with the benzimidazole compounds. For six who received appropriate chemotherapy, treatment has been discontinued for an average of 4.6 years (range, 3-7 years) without an increase lesion size or other evidence of reactivation.

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Section: Methodsmentioning

confidence: 99%

Parasiticidal Effect of Chemotherapy in Alveolar Hydatid Disease: Review of Experience with Mebendazole and Albendazole in Alaskan Eskimos

Wilson

Rausch

McMahon

et al. 1992

Clinical Infectious Diseases

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114

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“…Amman and Eckert [17] advised that a needle biopsy should be avoided in AEL patients, as it may cause metastases at the puncture site. Rausch and Wilson [18] and Ohnishi et al [19] injected minced material or homogenated and filtered fragments of solid lesions derived from AEL patients into rats or hamsters, respectively. Takagi et al [20] inoculated intracystic fluid into cotton rats, inducing metacestodes.…”

Section: Discussionmentioning

confidence: 99%

Intraperitoneal Dissemination Probably Caused by Needle Biopsy of Alveolar Echinococcosis of the Liver: Experimental Study

et al. 1997

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Alveolar echinococcosis of the liver (AEL) is a parasitosis with a potential for malignant tumor-like behavior. The disease is diagnosed by a combination of serologic tests, diagnostic images, and the histology of needle biopsy specimens. It remains unresolved whether the biopsy induces subsequent troubles. We designed this study to investigate critical problems after needle biopsy of AEL lesions using an experimental model. Five samples were prepared from the resected lesions of AEL patients: (A) 10% suspension of trypsin digests of the minced lesion; (B) 10% suspension of mesh-filtered sediment of the minced lesion; (C) 10% sediment suspension after washing the nonminced lesion; (D) supernatant after centrifuging intracystic fluid; (E) 10% sediment suspension after centrifuging intracystic fluid. A 1-ml aliquot of each sample was injected intraperitoneally into jirds (gerbils) or cotton rats, respectively. The animals were sacrificed 12 weeks later, and intraperitoneal metacestodes were observed. All samples except D developed metacestodes, and their histologies were all lesions of typical alveolar echinococcosis. These results suggest that a needle biopsy may cause intraperitoneal dissemination or tracial implantation of the parasites along the track of the needle.

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Alveolar Hydatid Disease in Minnesota

Gamble¹

1979

JAMA

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A 56-year-old woman from southwestern Minnesota underwent an extended left hepatic lobectomy to remove a large multinodular mass with a necrotic central cavity. The clinical, serological, and pathological findings led to the diagnosis of alveolar hydatid disease, and specific identification of Echinococcus multilocularis was achieved by growing mature larvas in voles inoculated intraperitoneally with tissue from the hepatic lesions. The patient probably acquired her infection some years previously from pet cats or dogs that had become infected by ingesting infected rodents. In North America E multilocularis is enzootic in the northern tundra zone of Alaska and Canada. Since 1964 the cestode has been recognized with increasing frequency in several north-central states, including Minnesota. The parasite may extend its range farther south, since suitable animal hosts occur throughout the United States.

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Rearing of the Adult Echinococcus Multilocularis Leuckart, 1863, From Sterile Larvae From Man

Cited by 18 publications

References 0 publications

Parasiticidal Effect of Chemotherapy in Alveolar Hydatid Disease: Review of Experience with Mebendazole and Albendazole in Alaskan Eskimos

Parasiticidal Effect of Chemotherapy in Alveolar Hydatid Disease: Review of Experience with Mebendazole and Albendazole in Alaskan Eskimos

Intraperitoneal Dissemination Probably Caused by Needle Biopsy of Alveolar Echinococcosis of the Liver: Experimental Study

Alveolar Hydatid Disease in Minnesota

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