Rearrangement and expression of the antigen receptor alpha, beta and gamma genes in suppressor antigen-specific T cell lines.

Abstract

The rearrangement and transcription of the antigen receptor alpha, beta and gamma genes were investigated in murine antigen‐specific suppressor T cell lines, to establish whether the suppressor T cell subset expresses the same antigen receptor transcripts previously found in helper and cytotoxic T lymphocytes. The genomic organization of the alpha, beta and gamma chain loci was investigated using probes representative of the entire gene or fragments from variable, joining and constant regions. The present results show that in functional suppressor T cells the three antigen receptor genes are all rearranged. The beta gene is expressed in all the tested cell lines, while the expression of the alpha and gamma genes is variable. In one cell line (LH8) alpha and gamma genes are not efficiently transcribed; in the other cell line (LA41) the gamma mRNA is found in amounts similar to beta mRNA, whereas the alpha gene is expressed at low levels. These data suggest that in suppressor T cells no direct correlation exists between the expression of alpha, beta and gamma antigen receptor genes and the effector function.

A Rad LV‐Induced γδ T Cell Lymphoma Displaying an Antitumoral Cytotoxicity

“…Finally, 7 genes are rearranged in the four cell lines studied but related mRNAs are found only in MP2 and NS8 cells. LH8 cells do not transcribe the 7 gene [24]. Cell surface immunostaining with specific MoAb demonstrated the presence of the jS gene products only on NS8 cells.…”

“…Although the NS8 and NS5 cell lines were induced according to the same procedure, they do not show the same restriction pattern. The LH8 and MP2 lines express 1.3 kb 0 RNA transcript ( [24]; this paper), derived from fully assembled V/9, D/3, J0 gene segments and are recognized by MoAb directed against tbe a0-TCR chains. On the opposite, the cytolytic cell line NS8 only presents a partial 0 rearrangement (3/32 is rearranged, C/3 is not) in association with too short /3 chain transcript.…”

“…The results reported are the mean of three experiments, DNA and RNA analysis. DNAs from cell culture pellets, liver or kidney tissues were extracted as reported previously [24]. Restriction endonuclease-digested DNAs were sizeseparated on 0.8% (w/v) agarose gels in 40 mM tris acetate, 2 mM EDTA.…”

“…Isolation of polyadenylated RNA, denaturating gel electrophoresis and blotting were performed as described previously [24]. Prehybridization, hybridization with the radioactive probes and washings of the blots were realized by the procedures described above for DNAs.…”

Sequence and expression of transcripts of the T-cell antigen receptor alpha-chain gene in a functional, antigen-specific suppressor-T-cell hybridoma.

“…However, the subunit composition ofthe antigen receptor on suppressor T cells (Ts) remains to be solved; several investigators have reported discrepant results, demonstrating that some Ts lines and hybridomas express a-and p-chain genes, whereas some do not (5)(6)(7)(8)(9) despite having their Ts function. Thus, two points should be discussed in this regard.…”