Rearrangement of a Large Novel Pseudomonas aeruginosa Gene Island in Strains Isolated from a Patient Developing Ventilator-Associated Pneumonia

Singh, Gaurav; Srinivasan, Ramya; Cheng, Jan‐Fang; Peng, Ze; Fujimura, Kei E.; Baek, Marshall; Panzer, Ariane R.; Tringe, Susannah G.; Chen, Fuhong; Sorek, Rotem; Weng, Li; Bristow, Jim; Wiener-Kronish, J. P.; Lynch, Susan V.

doi:10.1128/jcm.01626-13

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…Strikingly, AggR appears to control the expression of a number of transposases and transposon remnants (Tables and ) and putative AggR‐dependent promoters are located upstream of these transcription units (Tables and ). This suggests that genomic rearrangements, on both the chromosome and the pAA2 plasmid, may occur more frequently in EAEC 042 during the initiation of the AggR virulence programme and lead to genome evolution, something which has been observed in other bacterial species (Lindsay, ; Singh et al , ; Wan et al , ).…”

Section: Discussionmentioning

confidence: 80%

Organization and architecture of AggR‐dependent promoters from enteroaggregative Escherichia coli

Yasir

Icke

Abdelwahab

et al. 2018

Molecular Microbiology

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Summary Enteroaggregative Escherichia coli (EAEC), is a diarrhoeagenic human pathogen commonly isolated from patients in both developing and industrialized countries. Pathogenic EAEC strains possess many virulence determinants, which are thought to be involved in causing disease, though, the exact mechanism by which EAEC causes diarrhoea is unclear. Typical EAEC strains possess the transcriptional regulator, AggR, which controls the expression of many virulence determinants, including the attachment adherence fimbriae (AAF) that are necessary for adherence to human gut epithelial cells. Here, using RNA‐sequencing, we have investigated the AggR regulon from EAEC strain 042 and show that AggR regulates the transcription of genes on both the bacterial chromosome and the large virulence plasmid, pAA2. Due to the importance of fimbriae, we focused on the two AAF/II fimbrial gene clusters in EAEC 042 (afaB‐aafCB and aafDA) and identified the promoter elements and AggR‐binding sites required for fimbrial expression. In addition, we examined the organization of the fimbrial operon promoters from other important EAEC strains to understand the rules of AggR‐dependent activation. Finally, we generated a series of semi‐synthetic promoters to define the minimal sequence required for AggR‐mediated activation and show that the correct positioning of a single AggR‐binding site is sufficient to confer AggR‐dependence.

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Section: Discussionmentioning

confidence: 80%

Organization and architecture of AggR‐dependent promoters from enteroaggregative Escherichia coli

Yasir

Icke

Abdelwahab

et al. 2018

Molecular Microbiology

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“…Accumulation of resistance traits is more likely to occur in isolates with enhanced virulence [3,34], during infection and treatment, and hence P. aeruginosa selectively evolves resistance to antibiotic-mediated killing [10]. In spite of the significant genetic variability within the species, resistant clones that spread globally have been identified [103,104,105].…”

Section: Introductionmentioning

confidence: 99%

Correlation between phenotypic antibiotic susceptibility and the resistome in Pseudomonas aeruginosa

Jaillard

Belkum

Cady

et al. 2017

International Journal of Antimicrobial Agents

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Genetic determinants of antibiotic resistance (AR) have been extensively investigated. High-throughput sequencing allows for the assessment of the relationship between genotype and phenotype. A panel of 672 Pseudomonas aeruginosa strains was analysed, including representatives of globally disseminated multidrug-resistant and extensively drug-resistant clones; genomes and multiple antibiograms were available. This panel was annotated for AR gene presence and polymorphism, defining a resistome in which integrons were included. Integrons were present in >70 distinct cassettes, with In5 being the most prevalent. Some cassettes closely associated with clonal complexes, whereas others spread across the phylogenetic diversity, highlighting the importance of horizontal transfer. A resistome-wide association study (RWAS) was performed for clinically relevant antibiotics by correlating the variability in minimum inhibitory concentration (MIC) values with resistome data. Resistome annotation identified 147 loci associated with AR. These loci consisted mainly of acquired genomic elements and intrinsic genes. The RWAS allowed for correct identification of resistance mechanisms for meropenem, amikacin, levofloxacin and cefepime, and added 46 novel mutations. Among these, 29 were variants of the oprD gene associated with variation in meropenem MIC. Using genomic and MIC data, phenotypic AR was successfully correlated with molecular determinants at the whole-genome sequence level.

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“…We utilized sequencing data from 114 P. aeruginosa strains from different patients, sequenced at multiple time points during chronic infections, previously curated from the literature ( 7 ). While the experimental methods for culturing and sequencing the samples differed between various studies, all samples were derived from sputum samples, except for two strains whose samples were derived from endotracheal and bronchial aspirate, respectively ( 71 , 72 ). Isolates were clonally purified from clinical specimens and were mostly sequenced by short-read sequencing, except for the study by Wang et al ( 32 ), in which the isolates underwent sequencing by long reads using PacBio RS II.…”

Section: Methodsmentioning

confidence: 99%

Convergent Within-Host Adaptation of Pseudomonas aeruginosa through the Transcriptional Regulatory Network

et al. 2023

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Rearrangement of a Large Novel Pseudomonas aeruginosa Gene Island in Strains Isolated from a Patient Developing Ventilator-Associated Pneumonia

Cited by 10 publications

References 36 publications

Organization and architecture of AggR‐dependent promoters from enteroaggregative Escherichia coli

Organization and architecture of AggR‐dependent promoters from enteroaggregative Escherichia coli

Correlation between phenotypic antibiotic susceptibility and the resistome in Pseudomonas aeruginosa

Convergent Within-Host Adaptation of Pseudomonas aeruginosa through the Transcriptional Regulatory Network

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