Reassessment of MLST schemes for Leptospira spp. typing worldwide

Varni, Vanina Delia; Ruybal, Paula; Lauthier, Juan José; Tomasini, Nicolás; Brihuega, Bibiana; Koval, Ariel; Caimi, Karina

doi:10.1016/j.meegid.2013.08.002

Cited by 43 publications

(42 citation statements)

References 33 publications

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“…Among these strains, serovars Bataviae, Grippotyphosa, and Pyrogenes consisted of three different STs. The present study was focused mainly on those selected strains that are the most common serovars in China; further, the STs associated with evolutionary information were taken into account (Varni et al, 2013). …”

Section: Resultsmentioning

confidence: 99%

A Novel Pan-Genome Reverse Vaccinology Approach Employing a Negative-Selection Strategy for Screening Surface-Exposed Antigens against leptospirosis

Zeng

Wang

et al. 2017

Front. Microbiol.

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Reverse vaccinology (RV) has been widely used for screening of surface-exposed proteins (PSEs) of important pathogens, including outer membrane proteins (OMPs), and extracellular proteins (ECPs) as potential vaccine candidates. In this study, we applied a novel RV negative strategy and a pan-genome analysis for screening of PSEs from 17 L. interrogans strains covering 11 predominately epidemic serovars and 17 multilocus typing (MLST) sequence types (STs) worldwide. Our results showed, for instance, out of a total of 633 predicted PSEs in strain 56601, 92.8% were OMPs or ECPs (588/633). Among the 17 strains, 190 core PSEs, 913 dispensable PSEs and 861 unique PSEs were identified. Of the 190 PSEs, 121 were further predicted to be highly antigenic and thus may serve as potential vaccine candidates against leptospirosis. With the exception of LipL45, OmpL1, and LigB, the majority of the 121 PSEs were newly identified antigens. For example, hypothetical proteins BatC, LipL71, and the OmpA family proteins sharing many common features, such as surface-exposed localization, universal conservation, and eliciting strong antibody responses in patients, are regarded as the most promising vaccine antigens. Additionally, a wide array of potential virulence factors among the predicted PSEs including TonB-dependent receptor, sphingomyelinase 2, leucine-rich repeat protein, and 4 neighboring hypothetical proteins were identified as potential antigenicity, and deserve further investigation. Our results can contribute to the prediction of suitable antigens as potential vaccine candidates against leptospirosis and also provide further insights into mechanisms of leptospiral pathogenicity. In addition, our novel negative-screening strategy combined with pan-genome analysis can be a routine RV method applied to numerous other pathogens.

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Section: Resultsmentioning

confidence: 99%

A Novel Pan-Genome Reverse Vaccinology Approach Employing a Negative-Selection Strategy for Screening Surface-Exposed Antigens against leptospirosis

Zeng

Wang

et al. 2017

Front. Microbiol.

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“…Three major MLST schemes exist for Leptospira spp. typing worldwide, all supported by the online database http://pubmlst.org/leptospira/ [25,27,28]. MLST was attempted for glmU , pntA , sucA , tpiA , pfkB , mreA , and caiB genes, i.e.…”

Section: Methodsmentioning

confidence: 99%

Leptospira diversity in animals and humans in Tahiti, French Polynesia

et al. 2017

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BackgroundLeptospirosis is a highly endemic bacterial zoonosis in French Polynesia (FP). Nevertheless, data on the epidemiology of leptospirosis in FP are scarce. We conducted molecular studies on Leptospira isolated from humans and the potential main animal reservoirs in order to identify the most likely sources for human infection.Methodology/Principal findingsWild rats (n = 113), farm pigs (n = 181) and domestic dogs (n = 4) were screened for Leptospira infection in Tahiti, the most populated island in FP. Positive samples were genotyped and compared to Leptospira isolated from human cases throughout FP (n = 51), using secY, 16S and LipL32 sequencing, and MLST analysis. Leptospira DNA was detected in 20.4% of rats and 26.5% of pigs. We identified two Leptospira species and three sequence types (STs) in animals and humans: Leptospira interrogans ST140 in pigs only and L. interrogans ST17 and Leptospira borgpetersenii ST149 in humans and rats. Overall, L. interrogans was the dominant species and grouped into four clades: one clade including a human case only, two clades including human cases and dogs, and one clade including human cases and rats. All except one pig sample showed a unique L. interrogans (secY) genotype distinct from those isolated from humans, rats and dogs. Moreover, LipL32 sequencing allowed the detection of an additional Leptospira genotype in pigs, clearly distinct from the previous ones.Conclusions/SignificanceOur data confirm rats as a major potential source for human leptospirosis in FP. By contrast to what was expected, farm pigs did not seem to be a major reservoir for the Leptospira genotypes identified in human patients. Thus, further investigations will be required to determine their significance in leptospirosis transmission in FP.

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“…The obtained allele profiles were compared to the data sets of the three Leptospira MLST schemes (7–9) available at the PubMLST repository (https://pubmlst.org/) and resulted in sequence type 58 (ST58), ST140, and ST52, respectively. These STs have been previously identified in Leptospira interrogans serovar Pomona strains worldwide and therefore corroborate the identification of strain GR5.…”

Section: Genome Announcementmentioning

confidence: 99%