Reassessment of the antioxidative mixture for the challenging electrochemical determination of dopamine, noradrenaline and serotonin in microdialysis samples

Schoors, Jolien Van; Lens, Charlotte; Maes, Katrien; Michotte, Yvette; Smolders, Ilse Julia; Eeckhaut, Ann Van

doi:10.1016/j.jchromb.2015.06.010

Cited by 19 publications

(9 citation statements)

References 47 publications

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“…Samples (30 µl each) were automatically collected from each mouse using the CMA 142 microfraction collector, every 30 min, till the end of the experiment. To decrease the rate of 5-HT oxidation, each sample collection tube was pretreated with antioxidative agents (8 µl), containing 100 mM acetic acid, 0.27 mM disodium EDTA, and 12.5 μM ascorbic acid (pH 3.2) 83 , and the interstitial fluid 5-HT levels were measured immediately using high performance liquid chromatography with electrochemical detection. Data were analyzed using atwo-tailed Student's t-test or repeated measures one-way ANOVA.…”

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confidence: 99%

Acute EPA-induced learning and memory impairment in mice is prevented by DHA

et al. 2020

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Eicosapentaenoic acid (EPA), an omega-3 fatty acid, has been widely used to prevent cardiovascular disease (CVD) and treat brain diseases alone or in combination with docosahexaenoic acid (DHA). However, the impact of EPA and DHA supplementation on normal cognitive function and the molecular targets of EPA and DHA are still unknown. We show that acute administration of EPA impairs learning and memory and hippocampal LTP in adult and prepubescent mice. Similar deficits are duplicated by endogenously elevating EPA in the hippocampus in the transgenic fat-1 mouse. Furthermore, the damaging effects of EPA are mediated through enhancing GABAergic transmission via the 5-HT6R. Interestingly, DHA can prevent EPA-induced impairments at a ratio of EPA to DHA similar to that in marine fish oil via the 5-HT2CR. We conclude that EPA exhibits an unexpected detrimental impact on cognitive functions, suggesting that caution must be exercised in omega-3 fatty acid supplementation and the combination of EPA and DHA at a natural ratio is critical for learning and memory and synaptic plasticity.

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confidence: 99%

Acute EPA-induced learning and memory impairment in mice is prevented by DHA

et al. 2020

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“…Samples were collected at 0.5 h, 2 h, and 3 h of the whole microdialysis experiment. One part of the antioxidative mixture (100 mM acetic acid, 3.3 mM L -cysteine, 0.27 mM Na 2 EDTA, 12.5 μM ascorbic acid, pH 3.2) is added to four parts of dialysate ( Van Schoors et al, 2015 ), which was immediately stored at -20°C until DA and its metabolites measurement by HPLC.…”

Section: Methodsmentioning

confidence: 99%

TRH Analog, Taltirelin Improves Motor Function of Hemi-PD Rats Without Inducing Dyskinesia via Sustained Dopamine Stimulating Effect

Zheng

Chen

Tan

et al. 2018

Front. Cell. Neurosci.

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Thyrotropin-releasing hormone (TRH) and its analogs are able to stimulate the release of the endogenic dopamine (DA) in the central nervous system. However, this effect has not been tested in the Parkinson’s disease (PD), which is characterized by the DA deficiency due to the dopaminergic neurons loss in the substantia nigra. Here, we investigated the therapeutic effect of Taltirelin, a long-acting TRH analog on 6-hydroxydopamine-lesioned hemi-Parkinsonian rat model. 1–10 mg/kg Taltirelin i.p. administration significantly improved the locomotor function and halted the electrophysiological abnormities of PD animals without inducing dyskinesia even with high-dose for 7 days treatment. Microdialysis showed that Taltirelin gently and persistently promoted DA release in the cortex and striatum, while L-DOPA induced a sharp rise of DA especially in the cortex. The DA-releasing effect of Taltirelin was alleviated by reserpine, vanoxerine (GBR12909) or AMPT, indicating a mechanism involving vesicular monoamine transporter-2 (VMAT-2), dopamine transporter (DAT) and tyrosine hydroxylase (TH). The in vivo and in vitro experiments further supported that Taltirelin affected the regulation of TH expression in striatal neurons, which was mediated by p-ERK1/2. Together, this study demonstrated that Taltirelin improved motor function of hemi-PD rats without inducing dyskinesia, thus supporting a further exploration of Taltirelin for PD treatment.

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“…Subsequently, ASS234 was injected s.c. in a dose of 5 ( n = 7), 15 ( n = 9) or 30 ( n = 7) mg/kg. Doses and route of administration are based on previous work . Nine more samples were collected during 3 h after administration of the compound.…”

Section: Methodsmentioning

confidence: 99%

“…The day after surgery, microdialysis samples were collected with a temporal resolution of 20 min and 10 ll of an antioxidative mixture (100 mM acetic acid, 0.27 mM EDTA and 12.5 lM ascorbic acid) was added to 40 ll microdialysate. [27] Six baseline samples were collected, followed by a sham injection of 500 ll 0.9% NaCl (saline) s.c. and another six dialysis collections. Subsequently, ASS234 was injected s.c. in a dose of 5 (n = 7), 15 (n = 9) or 30 (n = 7) mg/kg.…”

Section: Microdialysis Experimentsmentioning

confidence: 99%