Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases

Effect on Broiler Production Performance and Meat Quality of Feeding Ulva lactuca Supplemented with Carbohydrases

“…A similar effect was recently reported when broilers were fed 15% of L. digitata combined with a recombinant alginate lyase [28]. The present results may indicate that the ulvan lyase partially disrupted the ulvan gel-forming structure composing the U. lactuca cell wall, as previously observed in vitro [21], with a consequent reduction in digesta viscosity caused by the presence of seaweed. However, this activity of ulvan lyase was not reflected in an improvement of broilers growth performance, which suggests a minor effect of the recombinant CAZyme on feed passage and nutrient digestibility [40].…”

“…The increase in n-3 PUFA caused by combining U. lactuca with ulvan lyase indicates a degradation of alga with consequent release of nutritional and bioactive compounds. An effect of the recombinant CAZyme on releasing fatty acids from U. lactuca biomass was recently demonstrated in vitro, but only some MUFA, particularly C18:1c9, were significantly released [21]. The enrichment of poultry meat with n-3 long-chain PUFA can have various human health benefits, such as the diminishment of atherosclerosis and cardiovascular diseases [54,55].…”

“…After 21 days of animal feeding with an adapted maize and soy-based diet, and during the finishing period, broilers were fed ad libitum one of 4 dietary treatments (n = 10) for 14 days: (1) maize and soy-based diet (control); (2) control with 15% U. lactuca powder (Algolesko; Plobannalec-Lesconil, Brittany, France) (UL); (3) UL diet added with 0.005% commercial CAZyme mixture (ULC); and (4) UL diet added with 0.01% ulvan lyase (ULE). The commercial carbohydrase mixture (Rovabio ® , Adisseo; Antony, France) contained, as main active enzymes, endo-1,3(4)-β-glucanase (EC 3.2.1.6) (4300 U/g) and endo-1,4-β-xylanase (EC 3.2.1.8) (3200 U/g), whereas ulvan lyase is a recombinant CAZyme (PL25 family) capable of releasing reducing sugars (4.54 g/L) and mono-and oligosaccharides (22.6 mmol /100 g dried alga) from U. lactuca biomass [21]. The mash experimental diets were formulated to be isocaloric and isonitrogenous and their ingredients are shown in Table 1.…”

“…biomass for protein and carbohydrate extractions [18][19][20]. In addition, the in vitro ability of a single ulvan lyase from the family of 25 polysaccharide lyases (PL25) to partially disrupt the U. lactuca cell wall and release mono-and oligosaccharides, and monounsaturated fatty acids (e.g., C18:1c9), was recently reported [21]. Furthermore, CAZymes have shown activity towards carbohydrate present in microalgae cell walls [22,23] and in grains [24,25].…”

“…In the present study, we hypothesized that the combination of a previously in vitro tested ulvan lyase (PL25) [21], or a commercial carbohydrase mixture with dietary U. lactuca replacing 15% of maize and soybean meal, increases the nutritive value of seaweed for broilers with a consequent improvement of meat quality through the deposition of health promoting bioactive compounds, without compromising animal growth performance.…”