Recellularization of auricular cartilage via elastase-generated channels

Lehmann, Johannes; Nürnberger, Sylvia; Narcisi, Roberto; Stok, Kathryn S.; Eerden, Bram C.J. van der; Koevoet, Wendy; Kops, Nicole; Berge, Derk ten; Osch, Gerjo J.V.M. van

doi:10.1088/1758-5090/ab1436

Cited by 11 publications

(8 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Decellularized tracheal matrices have attracted much attention in recent years because of their similar composition and microenvironments to natural tracheal cartilage 7a. However, the dense tracheal cartilage matrix makes it difficult for original cellular materials to be detached from or for new cells to grow into the matrix 20. Additionally, the decellularized tracheal matrix is difficult to produce in bulk due to donor shortages 21.…”

Section: Discussionmentioning

confidence: 99%

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Duan

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Wharton's jelly (WJ) is considered a potential scaffold in tissue‐engineered trachea for its similar composition and function to cartilage tissue. However, the feasibility of using WJ to construct engineered neocartilage tissue has not been reported, let alone tubular tracheal cartilage regeneration and segmental tracheal lesion repair. Here, electrospun nanofibrous membranes composed of three different decellularized WJ matrix (DWJM)/poly(ε‐caprolactone) (PCL) ratios (8:2, 5:5, and 2:8) are fabricated. The results demonstrate improved degradation speed, absorption, and cell adhesion capacity but weakened mechanical properties with increased DWJM content, but satisfactory homogeneous cartilage regeneration is only achieved in the DWJM/PCL (8:2) group after 12 weeks in vivo culture. Furthermore, homogeneous, 3D, tubular, trachea‐shaped cartilage is constructed with a controllable lumen diameter and wall thickness based on the 2D nanofibrous membrane using a modified sandwich model, in which the chondrocyte‐membrane construct is rolled around a silicon tube. Most importantly, by combining the above schemes with previously established vascularization and epithelialization techniques, chondrification, vascularization, and epithelialization are achieved simultaneously thus realizing long‐term (6 months) circumferential tracheal lesion repair in a rabbit model with a biological structure and function similar to that of native trachea, representing a promising approach for the clinical application of tracheal tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 99%

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Duan

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…In recent years, some researchers have developed a new type of enzymatic perforated bovine/human auricle cartilage ECM, which mainly digested elastic fibers in ear cartilage ECM by elastase, and created a channel network, in which the recellularized cells could migrate to the internal matrix and secrete glycosaminoglycan in situ to produce cartilage-like tissues. [64,65] In addition, the preservation of the vasculature is also one of the difficulties in constructing functional tissue engineered ears. Duisit et al [66] performed perfusiondecellularization on 12 human ears with vascular pedicles, and obtained the ECM with a preserved auricular vascular tree for the first time.…”

Section: Earmentioning

confidence: 99%

“…Although several efforts have been dedicated to reconstruct cartilage, the acellular cartilage matrix is very dense, which limits the inward growth of cells and hinders the recellularization and recovery of defective cartilage functions. In recent years, some researchers have developed a new type of enzymatic perforated bovine/human auricle cartilage ECM, which mainly digested elastic fibers in ear cartilage ECM by elastase, and created a channel network, in which the recellularized cells could migrate to the internal matrix and secrete glycosaminoglycan in situ to produce cartilage-like tissues [64,65] …”

Section: Research Progress In Plastic and Reconstructive Surgerymentioning

confidence: 99%

“…Ear Elastase-generated channel Promote the effect of recellularization, while keeping the entire cartilage structure intact [64,65] Photo-crosslinkable cartilagederived hydrogel bioink Provide printability and structural and mechanical stability, and promote the activity and maturation of chondrocytes in bioprinted constructs.…”

Section: Optimization Of Decellularization Protocolmentioning

confidence: 99%

See 1 more Smart Citation

Application of decellularization-recellularization technique in plastic and reconstructive surgery

Shang

Wang

Zhen

et al. 2023

Chinese Medical Journal

View full text Add to dashboard Cite

In the field of plastic and reconstructive surgery, the loss of organs or tissues caused by diseases or injuries has resulted in challenges, such as donor shortage and immunosuppression. In recent years, with the development of regenerative medicine, the decellularization-recellularization strategy seems to be a promising and attractive method to resolve these difficulties. The decellularized extracellular matrix contains no cells and genetic materials, while retaining the complex ultrastructure, and it can be used as a scaffold for cell seeding and subsequent transplantation, thereby promoting the regeneration of diseased or damaged tissues and organs. This review provided an overview of decellularization-recellularization technique, and mainly concentrated on the application of decellularization-recellularization technique in the field of plastic and reconstructive surgery, including the remodeling of skin, nose, ears, face, and limbs. Finally, we proposed the challenges in and the direction of future development of decellularization-recellularization technique in plastic surgery.

show abstract

“…Cadaveric decellularized cartilage has for instance been explored after crosslinking for joint resurfacing 37 ; irradiated cadaveric costal or nasal cartilage is sometimes used for nose reconstructions 38,39 ; and devitalized frozen allografts are routinely used for meniscus replacement in the clinic 40 . While functionally effective, host cells cannot repopulate decellularized cartilage due to the extreme density of the collagen fibrillar network and glycosaminoglycans (GAGs) that constitute its extracellular matrix 41,42 . This could be particularly problematic in pediatric patients as they are rapidly growing, and an acellular graft would not undergo the natural process of expansion and maturation of living tissue.…”

Section: Introductionmentioning

confidence: 99%

A Translational Tissue Engineering Approach to Airway Reconstruction Leveraging Decellularized Meniscus and Cartilage Progenitor Cells

Gehret

Ghavimi

Dumas

et al. 2022

Preprint

View full text Add to dashboard Cite

Severe subglottic stenosis develops in over 20,000 infants per year and requires laryngotracheal reconstruction (LTR) to enlarge the airway by implanting autologous cartilage from a rib graft. However, young children often lack sufficiently sized costal cartilage resulting in increased donor site morbidity and operative time, as well as an elevated risk for airway restenosis necessitating revision surgery. To overcome these limitations, we have created a first-of-its-kind scaffold based on porcine meniscal cartilage decellularization (MEND) by selectively digesting the elastin and blood vessels uniquely present in the meniscus to create microchannels that support cellular re-invasion. Here we demonstrated that MEND can be fully recellularized in 3 days with ear-derived cartilage progenitor cells (eCPCs) and reaches structural and functional maturation suitable for implant within 3 weeks of chondrogenic differentiation, a time frame compatible with clinical translation, a first in airway tissue engineering. To further this therapy toward clinical translation, we validated the eCPCs-MEND grafts in a New Zealand white rabbit LTR model. Our results demonstrated airway expansion, graft re-epitheliazation, neocartilage formation, and integration with adjacent native laryngotracheal cartilage, notably at a higher degree than the standard of care of autologous costal cartilage. No instances of adverse events of extrusion, granulation, infection, or calcification were observed in any of the 38 rabbits of our 3 months study. These results demonstrate the feasibility of our translational tissue engineering approach to laryngotracheal reconstruction and could overcome the autograft-associated limitations in pediatric patients and a decrease the risk of invasive revision surgery.

show abstract

Recellularization of auricular cartilage via elastase-generated channels

Cited by 11 publications

References 66 publications

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair

Application of decellularization-recellularization technique in plastic and reconstructive surgery

A Translational Tissue Engineering Approach to Airway Reconstruction Leveraging Decellularized Meniscus and Cartilage Progenitor Cells

Contact Info

Product

Resources

About